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Showing: 41 - 60 of 178 results

  1. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...their devices, which makes it very difficult to adapt the device to one's own samples and limits the use...to as 'frugal science', existing components are adapted and combined with creativity and feedback from ...

  2. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...for assembling the barcoded gRNA library pool. (Adapted from Wong et al., 2016). With a list of gRNA...assembling barcoded combinatorial gRNA libraries. (Adapted from Wong et al., 2016). With CombiGEM and ...

  3. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...in optogenetics, opening up the possibility of adapting GFP lines for optogenetics experiments in specific...transcription activation domains. Both systems are easily adaptable to neuroscience applications, including optogenetics...

  4. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...packaging into virus-like particles. Feng Zhang’s lab adapted the native PEG10 RNA sequences to instead drive...delivering gene editing tools into cells. Image adapted from Segel et al. 2021.   Prime editing improvements...

  5. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...features and evolved PAM sites, making Cas9 an adaptable choice for many genome engineering endeavors. ...   Choosing a Cas While Cas9 may be the most adaptable Cas currently available, the other Cas proteins...

  6. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...few steps. More recently, CRISPR technology has adapted Golden Gate cloning for inserting the appropriate...single gRNA-expressing plasmid, but it can also be adapted to express multiple gRNAs. Addgene has two Golden...

  7. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ... the simplest CRISPR systems and the easiest to adapt to genome editing, but the introduction of type ...positions are indicated below each graph. Figure adapted from Kleinstiver et al., 2016. Until recently...

  8. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...polyadenylation (spA) signal. Figure and caption adapted from Hazan and Bester 2021.    Alternative ...Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity. Science. 2012;337(6096):816...

  11. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...MagLOV’s parent protein AsLOV2 has already been adapted into a variety of optogenetic tools, they’re optimistic...optimistic that MagLOV can be similarly adaptable into future magnetogenetic tools.  Embracing science...

  12. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...spreading within and between genomes, and have been adapted for a wide variety of applications - as mutagenesis...at two different incubation temperatures. Data adapted from Vo et al., 2020. In E. coli, the new streamlined...

  14. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...applications. The first non-Cas9 CRISPR protein adapted for genome engineering was Cas12a (formerly Cpf1...targeting flexibility. Cas13a (formerly C2c2) has been adapted for targeted RNA cleavage in mammalian cells. The...

  16. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...trafficked from the “source” to “destination”. Images adapted from Lee and Cheah et al. 2023 bioRxiv (A) and ...:GFP-iPAK4 at 3 or 6 h. Scale bar 5 µm. Images adapted from Lin et al., 2023 bioRxiv, under CC-BY-NC 4.0...

  17. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...efficiencies at different loci in U2OS cells. Image adapted from Yan et al. 2024 under a CC-BY license. ...transduction efficiency for the indicated ssAAV5s. Figure adapted from Serrano et al. 2023 under a CC-BY-NC-ND license...

  18. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...have been developed for a singular purpose (ex. adapter trimming, quality score analysis, k-mer counting...Java, R, etc.) in our pipeline. Crucially, this adaptable property gave us the freedom to use multiple open-source...

  19. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...the late 1980s, scientists even created a codon adaptation index (CAI) that is based on the codon usage ...10.1038/nrg3051 Sharp PM, Li W-H (1987) The codon adaptation index-a measure of directional synonymous codon...

  20. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...structure of the H1.8-GFP-bound nucleosome. Images adapted from Arimura et al. 2024 under CC-BY-NC-ND 4.0 ...3D map with 3.1 Å global resolution (E). Figure adapted from Abe & Lim, 2024 under CC-BY-NC-ND 4.0 license...
Showing: 41 - 60 of 178 results