...workshop. Specifically, Jo, Brook, and Angela are co-leading "Making a Greater Impact Using Clear and ...

.... In this method, the major capsid protein VP1 is co-expressed with a fusion protein containing VP2C and... research, which required protein compartments to co-localize multiple enzymes in a reaction pathway, ...

... firefly luciferase activity in the Co-IP compared of a control Co-IP indicates stronger interaction. ...catalytic domain from DNA methyltransferase, DNMT3A. Co-expression of this modified Cas9 along with a gRNA... new method called DULIP (dual luminescence-based co-immunoprecipitation) to overcome the limitations ...Firefly luciferase. The bait and prey plasmids are co-transfected and proteins are immunoprecipitated from...tet-inducible OsTIR1 plasmid is also available). Second, they co-transfected short-arm donor vectors containing mAID..., and Cas9 activator expressing plasmids that can co-express 1-8 gRNAs. Ordon et al., 2016 tested the ...

...because any potential employers, collaborators, or co-workers want to know that you are a well-rounded ...Maybe you demonstrated empathy for a collaborator or co-worker and showed that you really understood their...

... requires the creation of a stable cell line that co-expresses three components: dCas9 with an added ...location of interest  When these three components are co-expressed, the gRNA targets the dCas9 to the loci...

...different leaf infiltration methods Co-cultivation (Li et al., 2009): The co-cultivation of Agrobacterium with...

... DBDs (see Figure 2a) (4). These fusions are then co-expressed in a yeast strain that also contains a ...your proteins are not post-translationally modified, co-expression of the enzyme responsible for the modification...

... protein (e.g., GFP or luciferase) that is either co-transfected with the test plasmid or transfected ...how much plasmid DNA to use for any individual or co- transfection, should be determined empirically.  ...

...1.1 Affinity Frequently used for western blots, IP, co-IP, IF, flow -cytometry; can occassionally interfere...1.2 Affinity Frequently used for western blots, IP, co-IP, IF, flow -cytometry, but rarely used for purification...

...newest initiatives, DayCon was the brainchild of SITN co-director Steph Guerra, a 3rd year graduate student...

...nearly 10 years ago, lunch felt like a family meal. My co-founders actually were my family (Kenneth Fan is ...

...This post was co-authored by Susanna Stroik and Rachel Leeson. Here at Addgene, we like to help share...

...Associate Professor at the University of Toronto, co-founded the COVID-19 Resources Canada site. To date...

...enhances RNA-guided methylcytosine editing by TET1 by co-delivering TET1 and protein factors for linking methylcytosine...

...Agrobacterium to ensure that they are maintained during co-cultivation with the target plant species. Thus, ...

...Oliveira is a PhD student in Hematology from Brazil and co-founder of Bricking Science.      Additional Resources...

...for gene delivery. In 2018, he interviewed Addgene co-founder Melina Fan and former Associate Director ...

...expressing your gene of interest, all you have to do is co-transform pBLADEONLY. That way, a previously cloned...

...encoded H2O2 probe is pQE-30-HyPer3 (2) which he will co-express together with a plasmid encoding glycine ...

...study 31 rare diseases,” said Claudia OuYang, BeHEARD Co-Director in a release. The research teams studying...