... that I would mainly do over the price of a used car. Later, as I applied for jobs, I knew I should negotiate...Resources on Addgene.org Check out our careers page Watch our career videos  ...her data. Perhaps you both have an issue with childcare timing pickup. Whoever takes the earlier slot ...

..., including in clinically-relevant lines such as CAR-T cells. Plus, this method is fast! These editing...

... University. Just as computers, cell phones, and cars become more technologically advanced leaving earlier...

...Degron-Based bioPROTACs for Controlling Signaling in CAR T Cells. Cold Spring Harbor Laboratory, 17 Feb. 2024...https://doi.org/10.1186/s12896-017-0393-y. Engler, Carola, et al. “A Golden Gate Modular Cloning Toolbox ...

... degree prepared them for their career.  2. Are you looking for career advancement and growth? A master...is a tool that can help you advance your career, promote career growth, and/or switch your field of work...Advancing along a career path in these two fields looks different however. In industry the career ladder is larger...it’s possible.  In academia, the career ladder is smaller, but career growth is possible even if your ...the bench longer (or for your whole career) with a master’s. Career advancement for PhDs, both in academia...If you'd like more career development tips, take a look at the Addgene Science Career Guide eBook Addgene... Science Career Guide eBook.   Resources Resources on the Addgene blog Learn about career planning ...

...different types of science policy careers, there are many ways to enter this career trajectory. One common route...this many times throughout my short science policy career and this seemingly simple question is a moving ...options for achieving success in a science policy career with many opportunities to pivot and grow along... The most challenging part of jumping into this career path from academia is the uncertainty of it all...school, standing at the start of a science policy career was much more circuitous and unknown. In fact, ... and most importantly, how, of a science policy career.  Who does science policy? I’ve intentionally started...creates a rich and diverse workforce. Out of all the career spaces I explored for my post-PhD life, the science...

...shown, comparing GFP, wrmScarlet (mScarlet), and the new mScarlet3 and mScarlet-I3 constructs. Scale bar...hours. In order to use the latest mScarlet tags, mScarlet3 and mScarlet-I3, Oliver Hobert’s lab recently...4 protein, mScarlet3 was 50% brighter than the original worm-optimized wrmScarlet. mScarlet-I3, while ...like mScarlet are essential tools for visualizing biological processes in vivo. However, mScarlet’s slow... The team designed coding sequences for mScarlet3 and mScarlet-I3 that were codon-optimized for C. elegans...structural studies of E3 ligase cereblon Optimized mScarlet-based tags for live imaging in C. elegans CRISPR...s41467-024-52871-9. PMID 39406745. Optimizing mScarlet tags for live imaging in C. elegans By Alyssa ...

...Taking Steps to Achieve a Successful Career Career Advice from the Career Path Panel Two of the panels at ...Graduate Student Career Outcomes University websites do not currently publish career outcomes for PhDs... FOR Boston 2015 symposium, the Career Path Panel and the Early Career Researchers in Publishing panel...achieve success in their careers. The take-away message I took from the career panel was that there are... boosting awareness of various career opportunities through our career, networking, and management for...navigating the waters of career development. Publishing Panel The Early Career Researchers in Publishing...2015. I personally participated as a member of the Career Paths Panel on the second day of the symposium ...

...important for their career goals, and identified possible funding sources to help them carry out their plans...co-directing Mentor Circle Programs at BWH and providing career mentoring to several students from Spain and all... their country some years ago looking for their career improvement) and bright students that will be the...where millions of people hold a PhD, achieving our career goals can be a real challenge. On top of that, ...broader, first-hand vision of the challenges and career opportunities that truly exist outside their immediate...discovered that the challenges that I am facing in my career are common problems that many others have faced...challenge did not hinder them from advancing their careers and achieving success.” Pedro Gonzalez Menendez...

...gap-year position taken to transition to the next career stage (ex: grad school, med school, industry) ... the lab? Ask your questions—especially if it’s scary. I’ve been afraid to ask “dumb questions” and to... help you along your path. Learning about their career paths and goals helped me reflect on what I wanted...Informational interviews with people who have taken career paths that you would be interested in can also ... next steps! More than serving as resources for career advice and advancement, my labmates have been some...I go. This story by Dr. Erika Moore in Science Careers discusses the importance of building a network ...resources! Many online resources and articles about careers in science are geared towards grad students and...

...who you don’t look up to and who doesn’t care about your career development? To get a better idea of whether...non-academic careers?    Will you be encouraged to develop transferable skills by attending career events or... it will make a huge difference for your future career and life. You might see a lab head as choosing ...had experience writing, publishing, or discussing career opportunities with the lab head. Former lab members...questions about lab culture. Support for diverse career paths I hope by now you are aware that the majority...scientists will not actually follow the academic career path taken by their training lab mentors. ~80% ...and preparing for both academic and non-academic career paths. I can’t say this strongly enough - don’t...

...create a network of support to help with elder care, carpooling, cooking on nights when you can’t get home... are no easy answers to negotiating a demanding career in science with other interests and priorities,...under control.” Think again. Maybe you’ll need to care for an elderly family member, want to train for ...not. You want your family and friends to know you care, but they need to know that you have an important...will respect you if you show respect for your own career and it will teach them to respect their own responsibilities...less You can work at home sometimes, but do so carefully and with attention to those around you #4 Thou...alone You, alone, are not solely responsible for care and attention to your home and family unless you...

...enterprising early career scientists taking an active part in creating their own opportunities for career development...independent career organizations to get the grants and programs they need such as at the Postdoc Career Development...ensure that all scientists can have successful careers doing science in some form? There is a lot of talk.... They are not exposed to the vast non-academia career landscape and there is insufficient (or no) emphasis...Pennsylvania has the Penn Biotech Group and PBG Healthcare Consulting. I know of many schools that have ...administrators who are finding ways to provide new types of career preparedness training for their scientists. I have...worked with some great postdoc and grad student career offices in the last few years. However, some organizations...

...acidic environments.   Carbenicillin   Carbenicillin, colloquially known as carb, belongs to the penicillin...both carb and amp, due to their common degradation mechanism. How to choose between the two? Carb is generally...similar relationship as amp and carb (they are interchangeable). Like carb, spectinomycin is the more stable...synthesis as its mode of action (sound familiar?). Carb is degraded by beta-lactamase, just like amp is.... bacteria. So… why do some people still use amp? Carb is expensive (relative to amp), and for some applications...marginal. Fortunately, amp can easily be replaced with carb if satellite colonies are an issue. Pros: Very stable...eukaryotic selection, whereas other plasmids must carry both a bacterial and a mammalian antibiotic selection...

...away from calling non-academic careers and desk jobs “alternative careers.” Realistically, based on the...Visit our careers topic page Read our transferable skills guide Read blog posts about career readiness... Resources at Addgene.org Find careers at Addgene Watch out career videos Sign up for the Addgene quarterly... transition. In this installment of the Addgene career guide, we’ll think about what causes this culture... feeling of camaraderie again in a non-academic career. You might not be in a lab anymore, but you will...scientists is the most enjoyable aspect of their chosen career. You can confidently call yourself a Scientist...course, a “desk job” could entail any number of career paths--industry, nonprofit organization, law office...

...easily deliver their “cargo” to target cells and organisms, and when that cargo is designed to make you...tools. As scientists, we can manipulate what this cargo is and where it goes, turning these pesky germs ...Once inside the host cell, the virus unleashes its cargo – its own genome.   Fig. 1: Viral particle...Depending on the type of virus used, the DNA (or RNA) cargo will either permanently integrate into the host ...cells with a high efficiency and deposit a DNA/RNA cargo into their targets makes them attractive and tractable...viruses to target specific cell types and deliver any cargo you fancy (size limits do apply, however.) Why ...each virus allows you to infect cells and deliver cargo, it’s important to understand their differences ...

...happiness in their careers regardless of their end goals and believe the popularity of our career posts shows...into a new job or maybe to an entirely new career path, a career coach might be able to help you. Head Addgenie... Joanne Kamens explains what career coaches do and offers up some career coach suggestions. 8. A Match.... We’re incredibly happy to see that one of our career posts has made it to the top of the list this year...repair by non-homologous end joining (NHEJ). 7. Career Coaching for Scientists: Why and Where Do I Find...making them difficult to use with CRISPR as the cargo. Mary Gearing explains some of the clever ways to...Read the Most Recent Plasmids 101, CRISPR 101, and Career Posts Resources at Addgene Browse Our CRISPR ...

...self-care during the COVID-19 pandemic, check out Care For Your Coronavirus Anxiety and Taking Care of ...events. Download Addgene’s Science Career guide to learn more about career paths and things you can do to ...help you keep calm and science on. Practice self-care and stay connected Find time for exercise. Many...supporting local businesses (ex: purchasing a gift card to use later or ordering take-out) or volunteering...identify skills to work on for the next steps in your career. Update your resume and CV. Attend virtual workshops...Scientist is now open to families at home, the STEM career exploration site Inventing Heron has a radio reporter...Reproducibility for Everyone. Plan out new experiments carefully. Consider submitting a registered report, where...

...particular topic. Given Joanne’s expertise in science careers, women in STEM, work-life negotiation, and mentorship... refer to if they have questions about science careers, women in STEM, work-life negotiation, and mentorship...Reddit “Ask Me Anything” was appealing, but also scary. I do a lot of public speaking where I enjoy communicating... large number of scientist posters with serious career dilemmas. Some questions were about general advice...to transition out of academia, and other science career topics. However, many of the posts were about toxic...the really tough times. Getting on a good science career path can be challenging these days, but for many...and postdoc programs beginning to support diverse career path development--but the science trainees still...

...Go to a science or career seminar together and meet to debrief on the topic (career) or presentation style...Invite guests from a lab, career or industry of interest – learn about their career paths and get their advice... for 25 years. They helped one another navigate career changes and overcome barriers by sharing broader...mentoring groups through my work with the renowned Healthcare Businesswomen’s Association (HBA) Boston Chapter...commit to the group. The group can be one gender and career stage (e.g. all women, all men, all postdocs, all...compete for fastest to set up a coffee date or most cards collected. The next blog in this series will have...other as you transition to the next stage in your careers! Stay in touch…this group will be the core of your...