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  2. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ... for multicolor imaging across scales. Nat Commun 11, 239 (2020). Oliinyk, O.S., Shemetov, A.A., Pletnev...dynamics of protein-protein interactions. Nat Methods 11, 641-644 (2014). Filonov, G.S. & Verkhusha, V.V. ...

  3. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ... and our new cloning grade DNA service. We've had 11 blog posts on COVID-19 featuring plasmid tools from...

  5. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...Polstein LR & Gersbach CA, Nat Chem Biol 2015 Mar;11(3):198-200. SunTag system for single molecule imaging... Synthetic Biology Center have deposited a set of 11 phage integrases which act irreversibly on their ...basis for Gateway cloning. The combination of all 11 att pairs in series with unique spacer sequences ...pMemoryArray) gives a DNA sequence capable of recording 11 bits (1.375 bytes) of information. This leads to ...pMemoryArray.   Yang et al., Nat Methods 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Bioluminescent... Schmidl, et al. ACS Synth Biol. 2014 Nov 21;3(11):820-31. Inncredible new tools for epitope tagging...

  6. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ... editing enzymes. Nature Reviews Microbiology, 20(11), 641–656. https://doi.org/10.1038/s41579-022-00739...

  9. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...agarose gel electrophoresis. Methods Mol Biol. 2009;582:11-25. doi: 10.1007/978-1-60761-340-4_2. PMID: 19763938...concatemers in mammalian cells. J Gene Med. 2009 May;11(5):444-53. doi: 10.1002/jgm.1310. PMID: 19263463....

  10. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...of AAVs as a new tool for neuroanatomical tracing (11). With the ability to produce neuron specific transfection...therapy 18.2 (2007): 171-182. PubMed PMID: 17328683. 11. Wouterlood, Floris G., et al. "A fourth generation...

  13. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ... in human somatic cells. Nature Protocols 2007; 2(11): 2734-46. PubMed PMID: 18007609. Addgene Materials...Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. 10.1038/nmeth.3047...

  14. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...Proceedings of the National Academy of Sciences USA 112(11) (2015): 3570-5. PubMed PMID: 25733849. PubMed Central...PMC4115528. Find plasmids from this paper at Addgene. 11. Cress, Brady F., et al. “CRISPathBrick: Modular ...

  15. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...photoactivation-localization microscopy (PALM, also FPALM) (11). For PALM, a small area containing inactive PA-FPs...PubMed PMID:18727145. PubMed Central PMCID:PMC4007248. 11.Hess, Samuel T., Thanu PK Girirajan, and Michael ...

  18. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...Organisms in Fundamental Research. Front. Microbiol. 11-2020, 2021. DOI: 10.3389/fmicb.2020.607028  Riley...
Showing: 41 - 60 of 121 results