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AAV Titration by qPCR Using SYBR Green Technology

Type

Protocol

... = 1.59 x 10 11 molecules/μL To obtain a solution at 2 x 10 9 molecules/μL: 1.59 x 10 11 / 2 x 10 9 = ...
Gibson Assembly Protocol

Type

Protocol

...the mouse mitochondrial genome. Nature Methods , 7(11), 901–903. https://doi.org/10.1038/nmeth.1515 (Link...
Protocol - How to Run an Agarose Gel

Type

Protocol

... you want to load 1.0 μg in 10 μL, make 1.1 μg in 11 μL. Reference Page Top Index...
Virus Protocol - Generating Stable Cell Lines

Type

Protocol

...Sanjana NE, Shalem O, Zhang F. Nature Methods. 2014 Aug;11(8):783-4 (Link opens in a new window) . Last reviewed...
Isolating a Monoclonal Cell Population by Limiting Dilution

Type

Protocol

...Sanjana NE, Shalem O, Zhang F. Nature Methods. 2014 Aug;11(8):783-4. (Link opens in a new window) Last reviewed...
AAV Production in HEK293 Cells

Type

Protocol

...55 mL of heat-inactivated FBS and 5 mL of glutaGRO 11 mL of 200 mM L-alanyl-L-glutamine. Store at 4 °C....

Showing: 1 - 6 of 6 results