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...and resources References Dong D, Guo M, Wang S, Zhu Y, Wang S, Xiong Z, Yang J, Xu Z, Huang Z (2017) Structural...Inhibit DNA binding Example:  AcrIIA4 blocks Cas9’s interaction with the PAM site (Dong et al., 2017),...deletion could replicate when challenged with the S. islandicus CRISPR-Cas system. 5. Phage therapy Phage...doi.org/10.1016/j.cell.2017.07.037 Li C, Psatha N, Gil S, Wang H, Papayannopoulou T, Lieber A (2018) HDAd5/...

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...all SARS-CoV-2 protein coding genes in E. coli and S. cerevisiae expression vectors are available at Addgene... full length and truncated versions of SARS-CoV-2 S (spike protein) cloned into a CMV driven expression...containing SARS-CoV-2 protein coding genes in E. coli and S. cerevisiae expression vectors in late March. The ...Plasmids and Resources Page! References Fukushi S, Watanabe R, Taguchi F (2008) Pseudotyped Vesicular...

...Terry, F. E., Roberts, B. J., Rosenberg, A. S., & De Groot, A. S. (2022). In silico Immunogenicity Assessment...www.frontiersin.org/articles/10.3389/fimmu.2022.885672Piché, M.-S., Mounho-Zamora, B., LeSauteur, L., & Burns-Naas, ...Manufacturing to Preclinical Studies. In J. Bluemel, S. Korte, E. Schenck, & G. F. Weinbauer (Eds.), The ...

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...biotin acceptor site BirA, a biotin ligase a gRNA(s) targeting a single genomic location of interest  ... Number of loci targeted One Many Sample type(s) Cell lines Cell lines, primary cells Table 1: ...methods.   Applications of CAPTURE 2.0 CAPTURE 2.0’s increased rate of detecting chromatin interactions...they are a large cluster of enhancers. CAPTURE 2.0’s multiplexing and high resolution allowed the Xu Lab...Li K, Cao H, Ni M, Liu Y, Gu Z, Dickerson KE, Xie S, Hon GC, Xuan Z, Zhang MQ, Shao Z, Xu J (2017) In ...

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...sequence* T2A: (GSG) E G R G S L L T C G D V E E N P G P P2A: (GSG) A T N F S L L K Q A G D V E E N P G...Y A L L K L A G D V E S N P G P F2A: (GSG) V K Q T L N F D L L K L A G D V E S N P G P * (GSG) residues...cloning sites flanking the multicistronic element(s), or you could replace one of the reporter genes above...

... Xiuyuan Ou, et al. that characterized the spike (S) protein of SARS-CoV-2 (Ou et al., 2020). The authors...development of a new plasmid, pCMV14-3X-Flag-SARS-CoV-2 S, which is now easily available through Addgene and...References Chu H, Chan JF-W, Yuen TT-T, Shuai H, Yuan S, Wang Y, Hu B, Yip CC-Y, Tsang JO-L, Huang X, Chai...Sigmund CD, Meyerholz DK, Kirby P, Look DC, Perlman S (2006) Lethal Infection of K18-hACE2 Mice Infected...

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...reaction. The destination vector and entry vector(s) are placed in a single tube containing the Type IIS...silent point mutations at internal recognition site(s) thus eliminating these from your gene of interest...throughput capability. Engler C, Kandzia R, Marillonnet S. PLoS One 2008;3(11):e3647. doi: 10.1371/journal.pone...PubMed.   Golden Gate cloning. Engler C, Marillonnet S. Methods Mol Biol. 2014;1116:119-31. doi: 10.1007/...

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