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Showing: 41 - 60 of 79 results

  1. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...plasmids in standard growth strains range from 12-16 hours, but cultures with lower copy plasmids may ...

  3. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...nucleotides are represented equally, that’s only a 1/16 chance of having a PAM at a given site. While it ...

  4. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...cell lines (such as HEK293, which multiplies every 16 hours). So if you, for example, split cells on a ...

  5. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ....g., 30 °C), and limiting your growth time (e.g., 16-18 hours). Finding reference protocols There are ...

  9. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...mediated knock-ins in mammalian cells. J Biotechnol., 16(4): 387-390 (2020). 10.1016/j.jbiotec.2019.11.010...

  12. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ... COLM (clarity-optimized light-sheet microscopy) (16).  Both 3DISCO and CLARITY have relied on the use...

  19. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...guide RNAs for the CRISPR/Cas9 system. Genome Biol 16: . https://doi.org/10.1186/s13059-015-0784-0 Zetsche...

  20. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ... the March is not about Democrats or Republicans (16). This is a Pro-Science and Pro-Evidence march. Who...
Showing: 41 - 60 of 79 results