...legal complexities can arise when scientists are sharing their materials. Questions about intellectual property...material transfers. When Addgene entered the plasmid sharing space, we heard over and over again that the legal...were one of the biggest hurdles for scientific sharing. So we set out to streamline this process. We take...material) Affirmation that materials will only be shared with non-profit organizations Assurance that...

... of plasmid sharing  Interested in more facts about Addgene's 10 years of plasmid sharing? Check out this...breakthrough research and your commitment to scientific sharing. In the coming years, we will continue to improve...improve our services and work to make sharing even easier. We can't wait to see what you put on the next ...

...Addgenies Brook Pyhtila and Nicole Waxmonsky Resource sharing shortens the time needed to go from planning an... experiments. Addgene’s dedication to resource sharing has led us to develop a searchable and sortable...submitting their sequences (and plasmids!) so that this shared resource can continue to grow. This post was ...CRISPR Technologies Available Through Resource Sharing. 2016. In press. Additional Resources on the Addgene...

...more about alternative careers for scientists, and share cool science. Unfortunately, it never clicked for...scientists on Twitter: who are they? What do they share? And how they are connected? Here are the highlights...personal on Twitter. Or maybe scientist prefer to share links to research that isn’t behind paywalls. 5....Use Reddit for Science Communication 10 Ways to Share Your Science Why Scientists Should Give Twitter ...

...Video to Share Your Research 5 Reasons to Use Reddit for Science Communication 10 Ways to Share Your Science...struggles in the laboratory routine. We hope that sharing and laughing at these struggles will bring people...like to see portrayed in LEGO form, feel free to share with us at [email protected]. Who knows,...

...manuscript by ZZZ et al., describes ... The authors share a wealth of data on ... The language is ... The ...public on-line repository (like GitHub, Zenodo, Figshare, Triad) while the wet-lab resources, like constructs...passionate about open science, BIG data analysis and sharing whatever she knows with whoever cares to listen...the Addgene blog Find blog posts on scientific sharing Get tips on writing a review article Resources...

...best way to solve problems is to work together and share information broadly. “Otherwise we will all have... come up with discoveries on their own and only share what they think will get the research funded,” she...she learned more about Addgene. “The mission to share critical reagents such as plasmids and viral stocks...He’s also interested to see Addgene expand the sharing platform to other reagents in biology. Luk H. Vandenberghe...

...make the screen-sharing experience more interactive. The button is on the right of the Share Screen control...accessed by anyone at any time. This document can be shared and serve as a springboard for future discussion..., Denfeld BA, de Eyto E, Hampton SE, Keller PS, Sharma S, Lewis ASL, Weyhenmeyer GA, O’Reilly CM, Lofton...

...-C’) that give the feature its characteristic T-shape. The arrangement of the B-B’ and C-C’ palindromic...sequences (A-A’, B-B’, and C-C’ regions) and forms a T-shaped structure. The Rep binding element (RBE) and terminal...different AAV serotypes that vary in their RBE and T-shaped hairpin loop sequences (Wilmott et al. 2019). Most...10.1089/hgtb.2016.133. PMID: 28967288. Shitik, E. M., Shalik, I. K., & Yudkin, D. V. (2023). AAV- based vector...

...years, I've been looking forward to seeing how the sharing of his OSKM factors have affected the stem cell...engineering and stem cells, and the difficulty of sharing banked iPSCs across international borders. While...lost in the details. The speakers at this meeting shared plenty of details, but they also uniformly stepped...ground-breaking work on spinal cord injuries; Jessica Young shared research on stem cells and Alzheimer's Disease;...

...given by Phil Sharp. After a few rotations, Doench decided to complete his PhD work in Sharp’s lab.  Doench... easier for people everywhere…. the nonprofit, sharing ethic that Addgene has behind it is something that... sharing...

... maps and protocols! In reality, there are many shared components across viruses (AAV, lentivirus, etc...This means that lentivirus and gamma-retrovirus share a lot of core components, so we will discuss them...are slightly different in sequence. Both LTRs do share common repeat regions in addition to their unique...components Lentiviruses have gone through their fair share of lab evolution. There are 1st, 2nd, and 3rd generation...

...Scientific Writing.” In this post, we’d like to share parts of our workshop with you!  Accessibility  ...your readers are now a team, working together to share information. Sometimes it is most helpful to use...practice image. We know; we asked each group to share with the rest of the workshop.  For Figure 1, Addgene... will flow out the bottom syringe."Feel free to share your alt text in the comments! We learned a lot ...

...educational content Addgene truly believes that sharing speeds science, and our entire mission is to enable...informative content to help researchers in creating and sharing knowledge, related to the types of materials we...originally started as a way to easily store and share in-depth answers to frequently asked questions. ...licensed and cited figures from papers or image sharing repositories. Then we format, do a final review...

... connexin localized correctly to gap junctions (Shaner et al, 2008). Obligate dimers (such as dTomato)...assay to demonstrate monomeric behaviour in cells (Shaner et al, 2013; Bindels et al, 2017). Still, the data...knowledge and experience that is gathered can be shared by publishing it as (part of) figure 1, uploading...or cells or organisms that are engineered can be shared to simplify the comparison by others. Together,...

...proteins may display reversible intensity changes (Shaner et al, 2008; Bindels et al, 2017) and photoswitching...use widely varying amounts of excitation power (Shaner et al, 2008). In confocal laser scanning microscopy... concentration may affect photobleaching rates (Shaner et al, 2008). This brings us to the key question...does not follow a simple mono-exponential decay (Shaner et al, 2008; Bindels et al, 2017) and cannot be...

...scientists. You can find him on Twitter @krisshamloo and at krisshamloo.com     Additional Resources on the Addgene...for them and let them in on the fun too. Kris Shamloo is a Software Engineer at Addgene and is interested... Kris Shamloo...

...also don’t have to go back to school right away. Shannon Rinaldi, Addgene’s Lab Manager, was taking classes...stay in science.” Addgenies Beth Kenkel and Shannon Rinaldi in the Addgene lab! Read their Instagram... publish their research and are not so keen on sharing their results in fear of getting scooped, she said...side of science, she stuck with it. Lab Manager Shannon Rinaldi values loving what she does. Her parents...

...Interactions. Filonov GS, Verkhusha VV. Chem Biol. 2013 Vladislav Verkhusha iRFP GAF domains from miRFP709...Shcherbakova et al. Nat Commun. 2016 Vladislav Verkhusha Multiple FAST Fluorescence-activating and absorption...of protein-protein interactions in living cells. Shao S, Zhang H, Zeng Y, Li Y, Sun C, Sun Y. Commun Biol...

...can optimize your growth time, temperature, and shaking speed to maximize cell density and plasmid yield... total culture volume and you should ensure the shaker speed is fast enough for sufficient gas exchange...culture. Generally, large cultures in flasks may be shaken around 220 RPM, but smaller cultures, especially...especially those in deep-well plates, require a faster shaking speed for proper aeration (260-300 RPM). 37℃ doesn...

...research, the MoClo Kit she and Sylvestre Marillonnet share with the scientific community via Addgene, the struggles... it include? Patron: Sainsbury has a mandate to share and educate and enable plant science in general....editing plant genomes. We will definitely plan to share those tools with the plant community. We’d like ... whose tweets I find really inspirational. They share experiences, articles about women in science, and...

...If you're interested in sharing your science, one of the big questions you'll be faced with is "how?"...it works so well for you, of course you want to share it with others! After all, won't it work just as...for longer educational videos, and Twitter, for sharing updates, new deposits, and new blog posts!  ...  of proficiency.  What kind of content are you sharing?  Sometimes your subject matter will drive the ...

...When Karl Barry Sharpless was awarded this year’s Nobel Prize in Chemistry, he joined an elite club, ...mentors pass on more than just technical knowledge, shaping their trainees’ very way of thinking too. Albert...interest to other researchers in their field. Indeed, sharing results with the broader scientific community represents...serviceman in the U.S. Army, and hockey coach all shaped his teaching style, with the former offering excellent...

...other groups. First within my department where we shared materials and equipment between labs. Later I worked...PostDoc associations and specific projects where you share methods and equipment between lab groups and departments...twitter and maintain a connection by commenting and sharing. LinkedIn or ResearchGate allow you to send personal...events, articles and newsletters I make sure to share them with a short note - this creates a thoughtful...

...story. Figure 1: Addgene’s Agile Approach Sharing is caring There are many moving parts to our software...diversifying our own knowledge of the software, sharing knowledge allows for life to happen without impacting...only thing you need to make a cake. The flavor, shape, and texture of a cake depends on how you combine...and discovery. We work with transparency, gladly share our knowledge, and love to collaborate, which, incidentally...

...advantage of the EnvA-TVA receptor-envelope system (Wickersham et al., 2007). This system requires first supplying... and jump the synapse to the previous neuron (Wichersham et al., 2007). However, once rabies dG enters...https://doi.org/10.1128/jvi.71.8.6174-6178.1997 Wickersham IR, Finke S, Conzelmann K-K, Callaway EM (2006...Methods 4:47–49 . https://doi.org/10.1038/nmeth999 Wickersham IR, Lyon DC, Barnard RJO, Mori T, Finke S, Conzelmann...

...than DNA substrates. In terms of structure, Cas13a shares no homology to the most commonly used CRISPR enzymes...The Feng Zhang lab used Cas13a from Leptotrichia shahii (LshCas13a) to begin to characterize this new effector... the CRISPR field. We feel that the culture of sharing within the CRISPR community is an essential part...and as always, we appreciate the opportunity to share these invaluable reagents with the scientific community...

... Researchers have been sharing plasmids ever since there were plasmids to share. Back when I was in the...plasmids he wanted. Addgene makes it so much easier to share your constructs from lab to lab. Promega supports...innovative tools. Afterall, isn’t science better when we share? NanoLuc® Luciferase-based tools are fantastic ...

... says “internationalization” young hearts often shake with the fear of uncertainty. However, internationalization...exist outside their immediate frontiers. Moreover, sharing a culture/country of origin with an international... help them carry out their plans. Through their shared experiences, mentors and mentees often developed...significant. Doubts, fears, insecurities… all shrink when shared with a mentor who has experienced them before and...

...Using acrylamide instead of agarose gives much sharper bands. I compared two MOIs of gRNA transduction... fragments, run PAGE instead of agarose to get sharper bands. Conclusion Overall, making a human cell...available techniques and how quick and easy it is to share information and regents, it’s exciting to think ...genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. ...

...Throughout the party, we shared memories of Addgene's past. Framed photos and slideshows shared some of the moments...you all so much for an amazing twenty years of sharing science!       ...

...Creator: Tom McFadden Using Video to Share Your Science: We Share Science Additional Resources on Addgene.org...gained along the way to ourselves so we decided to share some of our tips in our new series: Addgene Lab ...

...that can help: Antibody data repositories, which share validation data, and antibody search engines, which...antibodies from many vendors, while data repositories share validation and experimental data to help you decide...application-specific ones to anything goes. We've shared the ones we're familiar with in Table 1.  Table...

... depositor, but it can also be valuable data to share in grant proposals or to demonstrate impact in annual...standardized way. Since Addgene encourages the public sharing of aggregated data only, information on requesting...you find the dashboard useful when analyzing and sharing your own depositor metrics!  About the Author Joachim...

..., developed and deposited at Addgene by Arlene Sharpe’s lab, enables in vivo screens in naïve T cells....Marty LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested..., Hacohen, N., Doench, J. G., LaFleur, M. W., & Sharpe, A. H. (2024). Framework for in vivo T cell screens...

...look forward to another great year of sharing!   References 1. Shalem, Ophir, et al. "Genome-scale CRISPR-Cas9...PMCID: PMC4089965. 2. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide...

... scientists from around the world and help them share their lab experiences, findings, and experiments...Neurodegenerative and brain disorders are frequently overshadowed by other medical conditions, so these topics...welcome researchers from all fields of science to share their experiences and explain what they study or...

...focused on our core mission to promote scientific sharing. Listen to learn more about Darrell, his research...practices and promotes open science in the lab - sharing reagents, cell lines, data, and knowledge without...Addgene board of directors - increasing open source sharing and helping us stick to our core mission. Chapter...

...popular and represent the diversity of tools we share on behalf of scientists. 9. Plasmids 101: Positive...Joachim Goedhart Guest blogger Joachim Goedhart shares some strategies for generating GFP fusion proteins...proteins. From considering protein size and shape, linker sequences, and fusion location, there are many ...

...as spook, spookier, phantom, disembodied, shadow, and shade. Tongue-eating louse Cymothoa exigua is a ...(yes, Blugene made an appearance there too) and shared fascinatingly creepy science stories. Here are ...