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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 626 results for: Dos

Showing: 561 - 580 of 626 results

  2. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ... become valuable tools for your research! If they do (or if they don't!), please consider sharing your...

  3. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ..., Freund E, Ricci MS, Carpenter JF, Lengsfeld CS. Do not drop: mechanical shock in vials causes cavitation....27776. Epub 2021 May 4. PMID: 33811659. Pace CN, Vajdos F, Fee L, Grimsley G, Gray T. How to measure and...

  4. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...ddPCR for AAV quantitation blog post.  We generally do not recommend using ELISA-based titering assays, ...determination of the median tissue culture infective dose (TCID50) (Zen et al., 2004).  To choose the optimal...

  7. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...Agrobacterium strains see Weigel and Glazebrook, 2002. Tips: Do a colony PCR on your agrobacterium: Getting a transformant...

  9. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ... regions communicate with each other and how they do it (i.e. where the signals come from and what implications...migrates through neurons where it can replicate and by doing so spread across several synaptic connections (Ugolini...

  10. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...to use the deaminated DNA strand as a template. To do so, they used a Cas nickase, instead of dCas9. The...editors often produce a mixed population of edits, ABEs do not display significant A to non-G conversion at ...

  15. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...less stable than viruses produced in cell lines that do not express these sugars. (Takeuchi et al., 1997)...

  16. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ... extension can usually ensure that the 3’-base(s) do not form secondary structures. The introduction (...

  17. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...science.1138389 Le TTH, Tran NT, Dao TML, Nguyen DD, Do HD, Ha TL, Kühn R, Nguyen TL, Rajewsky K, Chu VT ...off-target editing, and, for RNA editing, fine-tuning dosage so that enough wild-type MECP2 is made inside the...

  18. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...biorender.com.   Of course, this is quite easy to do on a plot generated in Biorender! While a helpful...

  19. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...allowing co-imaging. And because bioluminescent markers do not need to be excited by an external light source...
Showing: 561 - 580 of 626 results