We narrowed to 632 results for: Dos

...your research, be sure to consider how its features do and don’t support reproducibility as not all ELNs...

...Agrobacterium strains see Weigel and Glazebrook, 2002. Tips: Do a colony PCR on your agrobacterium: Getting a transformant...

...single molecules - something researchers often want to do at neuronal synapses for instance. Also these tags...

...systemic delivery of high doses of vectors are needed. Additionally, AAVs do not integrate in the host...

...your sample names and guide sequences, and ICE will do the rest. There are no parameters that need optimizing...

...virus (similar to lentiviral vectors) because they do not encode all of the necessary viral proteins. Minigenome...

...ddPCR for AAV quantitation blog post.  We generally do not recommend using ELISA-based titering assays, ...determination of the median tissue culture infective dose (TCID50) (Zen et al., 2004).  To choose the optimal...

...evaluate tens of thousands of designs in parallel. To do this, we performed high-throughput ‘self-targeting...

...high-quality data for our DNA-based reagents. Not only do we extract as much information as possible from depositing...

...protein molecules fail to correctly fold? Misfolded FPs do not fluoresce. A dark population is not readily apparent...

... list is a great place to start, the tables above do not delve into the tissue or development-specific...

...to use the deaminated DNA strand as a template. To do so, they used a Cas nickase, instead of dCas9. The...editors often produce a mixed population of edits, ABEs do not display significant A to non-G conversion at ...

... regions communicate with each other and how they do it (i.e. where the signals come from and what implications...migrates through neurons where it can replicate and by doing so spread across several synaptic connections (Ugolini...

...allowing co-imaging. And because bioluminescent markers do not need to be excited by an external light source...

...science.1138389 Le TTH, Tran NT, Dao TML, Nguyen DD, Do HD, Ha TL, Kühn R, Nguyen TL, Rajewsky K, Chu VT ...off-target editing, and, for RNA editing, fine-tuning dosage so that enough wild-type MECP2 is made inside the...

...machinery such as Rep or other initiator proteins, they do not require host proteins for replication and can...spp., Rhizobium spp., Rhodopseudomonas spp., Rhodospirillum spp., Shewanella spp., Thiobacillus spp., Xanthomonas...

...biorender.com.   Of course, this is quite easy to do on a plot generated in Biorender! While a helpful...

... extension can usually ensure that the 3’-base(s) do not form secondary structures. The introduction (...

...find a positive one. So if you have better things to do than clone picking, choose your guide RNAs wisely...

...clinical grade hiPSCs, reprogramming methods that do not involve modification of the original genome by...