We narrowed to 629 results for: Dos

1. Save Time with Transient Plant Leaf Transformations

   Type

   Blog Post

   Published

   July 25, 2019, 12:03 p.m.

   ...in the biosynthetic pathway. Why plant scientists do transient leaf transformations The development of...

2. Isolating Genomic Regions of Interest with the CRISPR System

   Type

   Blog Post

   Published

   May 30, 2019, 12:44 p.m.

   ...Hoshino and Fujii, 2009). Other forms of enChIP, that do not use dCas9, require Zinc-finger or TAL proteins...

3. Supporting Reproducibility with a Connected ELN

   Type

   Blog Post

   Published

   Jan. 17, 2019, 1:38 p.m.

   ...your research, be sure to consider how its features do and don’t support reproducibility as not all ELNs...

4. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

   Type

   Blog Post

   Published

   June 25, 2024, 1:15 p.m.

   ...evaluate tens of thousands of designs in parallel. To do this, we performed high-throughput ‘self-targeting...

5. High Complexity Golden Gate Assembly with NEB’s DAD

   Type

   Blog Post

   Published

   Aug. 1, 2023, 1:15 p.m.

   ... GetSet Need a set of overhangs, stat? GetSet can do that for you, complete with predicted fidelity. You...

6. Viral Vectors 101: Preparing Pooled Libraries

   Type

   Blog Post

   Published

   July 13, 2023, 1:15 p.m.

   ... pooled libraries are typically DNA preps, but we do distribute several of our most popular CRISPR pooled...

7. Bioinformatics at Addgene

   Type

   Blog Post

   Published

   July 14, 2022, 1:15 p.m.

   ...high-quality data for our DNA-based reagents. Not only do we extract as much information as possible from depositing...

8. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

   Type

   Blog Post

   Published

   March 17, 2016, 2:30 p.m.

   ...virus (similar to lentiviral vectors) because they do not encode all of the necessary viral proteins. Minigenome...

9. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

   Type

   Blog Post

   Published

   Oct. 27, 2015, 3 p.m.

   ...protein molecules fail to correctly fold? Misfolded FPs do not fluoresce. A dark population is not readily apparent...

10. Plasmids 101: The Promoter Region – Let's Go!

    Type

    Blog Post

    Published

    April 3, 2014, 8:05 p.m.

    ... list is a great place to start, the tables above do not delve into the tissue or development-specific...

11. CRISPR 101: Cytosine and Adenine Base Editors

    Type

    Blog Post

    Published

    Feb. 13, 2025, 2:15 p.m.

    ...to use the deaminated DNA strand as a template. To do so, they used a Cas nickase, instead of dCas9. The...editors often produce a mixed population of edits, ABEs do not display significant A to non-G conversion at ...

12. Using AAV for Neuronal Tracing

    Type

    Blog Post

    Published

    Aug. 9, 2018, 1:04 p.m.

    ... regions communicate with each other and how they do it (i.e. where the signals come from and what implications...migrates through neurons where it can replicate and by doing so spread across several synaptic connections (Ugolini...

13. Hot Plasmids: Fall 2024

    Type

    Blog Post

    Published

    Nov. 21, 2024, 1:30 p.m.

    ...allowing co-imaging. And because bioluminescent markers do not need to be excited by an external light source...

14. Site Directed Mutagenesis by PCR

    Type

    Blog Post

    Published

    Aug. 2, 2016, 2:30 p.m.

    ... extension can usually ensure that the 3’-base(s) do not form secondary structures. The introduction (...

15. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type

    Blog Post

    Published

    Sept. 5, 2017, 1:58 p.m.

    ...find a positive one. So if you have better things to do than clone picking, choose your guide RNAs wisely...

16. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type

    Blog Post

    Published

    Dec. 14, 2017, 2:08 p.m.

    ...clinical grade hiPSCs, reprogramming methods that do not involve modification of the original genome by...

17. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type

    Blog Post

    Published

    Feb. 22, 2017, 3:30 p.m.

    ...saturation mutagenesis with the same protocol. What do you think about one pot saturation mutagenesis? How...

18. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type

    Blog Post

    Published

    Nov. 19, 2020, 2:15 p.m.

    ...science.1138389 Le TTH, Tran NT, Dao TML, Nguyen DD, Do HD, Ha TL, Kühn R, Nguyen TL, Rajewsky K, Chu VT ...off-target editing, and, for RNA editing, fine-tuning dosage so that enough wild-type MECP2 is made inside the...

19. Plasmids 101: Stringent Regulation of Replication

    Type

    Blog Post

    Published

    June 20, 2024, 1:15 p.m.

    ...we can tell, DnaA doesn’t need to hydrolyze ATP to do its job — although ATP does get hydrolyzed over time...

20. Plasmids 101: Broad Host Range Plasmids

    Type

    Blog Post

    Published

    April 11, 2024, 1:15 p.m.

    ...machinery such as Rep or other initiator proteins, they do not require host proteins for replication and can...spp., Rhizobium spp., Rhodopseudomonas spp., Rhodospirillum spp., Shewanella spp., Thiobacillus spp., Xanthomonas...