We narrowed to 629 results for: Dos
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TypeBlog PostPublishedMay 30, 2019, 12:44 p.m....Hoshino and Fujii, 2009). Other forms of enChIP, that do not use dCas9, require Zinc-finger or TAL proteins...
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Supporting Reproducibility with a Connected ELN
TypeBlog PostPublishedJan. 17, 2019, 1:38 p.m....your research, be sure to consider how its features do and don’t support reproducibility as not all ELNs... -
Viral Vectors 101: Producing Your rAAV
TypeBlog PostPublishedJuly 16, 2024, 1:15 p.m....ddPCR for AAV quantitation blog post. We generally do not recommend using ELISA-based titering assays, ...determination of the median tissue culture infective dose (TCID50) (Zen et al., 2004). To choose the optimal... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog PostPublishedJune 25, 2024, 1:15 p.m....evaluate tens of thousands of designs in parallel. To do this, we performed high-throughput ‘self-targeting... -
Tips for arabidopsis transformation
TypeBlog PostPublishedOct. 25, 2018, 1:23 p.m....Agrobacterium strains see Weigel and Glazebrook, 2002. Tips: Do a colony PCR on your agrobacterium: Getting a transformant... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog PostPublishedAug. 9, 2016, 2:30 p.m....explore. I hope that this brief guide can help you do just that. Good luck, and always keep going. Appendix... -
CRISPR Antimicrobials
TypeBlog PostPublishedMay 3, 2016, 2:30 p.m....become resistant to? CRISPR may provide a method for doing just that. While challenges remain in the delivery... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog PostPublishedMarch 17, 2016, 2:30 p.m....virus (similar to lentiviral vectors) because they do not encode all of the necessary viral proteins. Minigenome... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog PostPublishedOct. 27, 2015, 3 p.m....protein molecules fail to correctly fold? Misfolded FPs do not fluoresce. A dark population is not readily apparent... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog PostPublishedApril 3, 2014, 8:05 p.m.... list is a great place to start, the tables above do not delve into the tissue or development-specific... -
Using AAV for Neuronal Tracing
TypeBlog PostPublishedAug. 9, 2018, 1:04 p.m.... regions communicate with each other and how they do it (i.e. where the signals come from and what implications...migrates through neurons where it can replicate and by doing so spread across several synaptic connections (Ugolini... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog PostPublishedFeb. 13, 2025, 2:15 p.m....to use the deaminated DNA strand as a template. To do so, they used a Cas nickase, instead of dCas9. The...editors often produce a mixed population of edits, ABEs do not display significant A to non-G conversion at ... -
Rett Syndrome: A History of Research and Therapeutic Outlooks
TypeBlog PostPublishedNov. 19, 2020, 2:15 p.m....science.1138389 Le TTH, Tran NT, Dao TML, Nguyen DD, Do HD, Ha TL, Kühn R, Nguyen TL, Rajewsky K, Chu VT ...off-target editing, and, for RNA editing, fine-tuning dosage so that enough wild-type MECP2 is made inside the... -
Filming Science Videos in the Age of Social Distancing
TypeBlog PostPublishedMay 20, 2021, 1:15 p.m....camera’s image to my remote location, live. After doing a bit of looking, I found an inexpensive HDMI transmitter... -
Viral Vectors 101: Pseudotyping
TypeBlog PostPublishedApril 20, 2021, 2:30 p.m....less stable than viruses produced in cell lines that do not express these sugars. (Takeuchi et al., 1997)... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog PostPublishedSept. 5, 2017, 1:58 p.m....find a positive one. So if you have better things to do than clone picking, choose your guide RNAs wisely... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog PostPublishedFeb. 22, 2017, 3:30 p.m....saturation mutagenesis with the same protocol. What do you think about one pot saturation mutagenesis? How... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog PostPublishedDec. 14, 2017, 2:08 p.m....clinical grade hiPSCs, reprogramming methods that do not involve modification of the original genome by... -
Hot Plasmids: Fall 2024
TypeBlog PostPublishedNov. 21, 2024, 1:30 p.m....allowing co-imaging. And because bioluminescent markers do not need to be excited by an external light source... -
Antibodies 101: Reading a Flow Plot
TypeBlog PostPublishedFeb. 6, 2024, 3:13 p.m....biorender.com. Of course, this is quite easy to do on a plot generated in Biorender! While a helpful...