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...Agrobacterium strains see Weigel and Glazebrook, 2002. Tips: Do a colony PCR on your agrobacterium: Getting a transformant...

...single molecules - something researchers often want to do at neuronal synapses for instance. Also these tags...

...systemic delivery of high doses of vectors are needed. Additionally, AAVs do not integrate in the host...

...your sample names and guide sequences, and ICE will do the rest. There are no parameters that need optimizing...

...was actually engineered to target the CNS. How well do these AAVs transduce the CNS and PNS? Both plasmids...from. Additionally, AAV.PHP.eB was used at a lower dose than it’s parent AAV.PHP.B: 1x1011 vs 1x1012 viral...blue (RGB) fluorescent protein (4a-c). When high doses of this viral cocktail are injected, by chance lots...the two green neurons near the arrows. When lower doses of virus are used in order to decrease the number...AAV.PHP.eB capsid. Both viruses were IV injected at a dose of 1x1011 vg per mouse and the level of transduction...AAV.PHP.S capsid. Both viruses were IV injected at a dose of 1x1012 vg per mouse and the level of transduction...-off transactivator (tTA), is injected at a high dose to maximize the number of cells that are labeled...

...explore. I hope that this brief guide can help you do just that. Good luck, and always keep going. Appendix...

...become resistant to? CRISPR may provide a method for doing just that. While challenges remain in the delivery...

...virus (similar to lentiviral vectors) because they do not encode all of the necessary viral proteins. Minigenome...

...protein molecules fail to correctly fold? Misfolded FPs do not fluoresce. A dark population is not readily apparent...

... list is a great place to start, the tables above do not delve into the tissue or development-specific...

...to use the deaminated DNA strand as a template. To do so, they used a Cas nickase, instead of dCas9. The...editors often produce a mixed population of edits, ABEs do not display significant A to non-G conversion at ...

... regions communicate with each other and how they do it (i.e. where the signals come from and what implications...migrates through neurons where it can replicate and by doing so spread across several synaptic connections (Ugolini...

...allowing co-imaging. And because bioluminescent markers do not need to be excited by an external light source...

...we can tell, DnaA doesn’t need to hydrolyze ATP to do its job — although ATP does get hydrolyzed over time...

...machinery such as Rep or other initiator proteins, they do not require host proteins for replication and can...spp., Rhizobium spp., Rhodopseudomonas spp., Rhodospirillum spp., Shewanella spp., Thiobacillus spp., Xanthomonas...

...science.1138389 Le TTH, Tran NT, Dao TML, Nguyen DD, Do HD, Ha TL, Kühn R, Nguyen TL, Rajewsky K, Chu VT ...off-target editing, and, for RNA editing, fine-tuning dosage so that enough wild-type MECP2 is made inside the...

...camera’s image to my remote location, live. After doing a bit of looking, I found an inexpensive HDMI transmitter...

...less stable than viruses produced in cell lines that do not express these sugars. (Takeuchi et al., 1997)...

...biorender.com.   Of course, this is quite easy to do on a plot generated in Biorender! While a helpful...

...clinical grade hiPSCs, reprogramming methods that do not involve modification of the original genome by...