We narrowed to 616 results for: Dos

Data-driven design of Golden Gate Assembly overhangs greatly increases the number of fragments that can be used in cloning.

Aug. 1, 2023, 1:15 p.m.

Pooled libraries are a powerful tool, but they can difficult to prepare! Learn the tips and tricks for taking your pooled libraries from plasmids to screening.

July 13, 2023, 1:15 p.m.

A recent study shows ancestral bias in CRISPR guide libraries, leading to skewed cancer dependency results, particularly in cell lines with African ancestry.

Nov. 29, 2022, 2:15 p.m.

Addgene uses bioinformations to streamline our sequencing and QC workflow. Learn how we built the process in this blog.

July 14, 2022, 1:15 p.m.

Find three ways to purify genomic DNA, plasmid DNA, or PCR products without a commercial kit. Also, find a method for regenerating silica columns for reuse.

Feb. 11, 2020, 2:15 p.m.

Transient plant leaf transformation can save you time... months in fact. Learn more about this method and get some tips and tricks for designing your transient plant leaf transformation experiment.

July 25, 2019, 12:03 p.m.

Read on to learn more about this technique to isolate specific genomic regions using CRISPR.

May 30, 2019, 12:44 p.m.

Electronic lab notebooks (ELNs) are useful tools that help researchers keep track of data. However, not all ELNs support the same functionality when it comes to reproducibility.

Jan. 17, 2019, 1:38 p.m.

Find tips for transforming arabidopsis with your plasmid construct of interest.

Oct. 25, 2018, 1:23 p.m.

Learn how you can use the Spaghetti Monster fluorescent protein (smFP) tags for neuronal tracing and multicolor labeling of brain structures.

Aug. 14, 2018, 12:42 p.m.

Learn how and why Adeno-associated virus (AAV) has enabled breakthroughs in retinal gene therapy.

July 31, 2018, 12:55 p.m.

Learn how you can use Synthego's ICE tool to analyze your CRISPR genome editing experiments.

May 8, 2018, 1 p.m.

Learn how the Gradinaru Lab at Caltech developed AAV vectors to target the peripheral and central nervous systems.

Sept. 28, 2017, 2:01 p.m.

Learn how to design a CRISPR screening experiment targeting enhancers.

Aug. 9, 2016, 2:30 p.m.

Antibiotic resistant bacteria are a huge issue in the clinic - learn how CRISPR is being developed to fight against antibiotic resistant super bugs.

May 3, 2016, 2:30 p.m.

Viral minigenomes provide a safe way to study dangerous viruses like Ebola. Read all about minigenomes inside.

March 17, 2016, 2:30 p.m.

Fluorescent proteins can mis-fold or form aggregates outside of the cytoplasm leading to errors in localization. Avoid these issues with new mOX-FPs.

Oct. 27, 2015, 3 p.m.

An overview of cytosine and adenine base transition editors, which make precise changes in DNA bases without causing double-strand breaks.

Feb. 13, 2025, 2:15 p.m.

Learn how AAVs can be used to trace neuronal connections.

Aug. 9, 2018, 1:04 p.m.

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

Nov. 21, 2024, 1:30 p.m.