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TypeBlog PostPublishedFeb. 23, 2023, 2:15 p.m....full-size, with assay-supported recommended uses and education content. This work exemplifies our long-standing...curated information about AAV usage in research applications. Scientists around the world can upload AAV ...accessed our freely available online collections of educational resources. In 2022 alone, our website saw 2.2...
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Making Career Development an Integral Part of the Science Training System
TypeBlog PostPublishedFeb. 25, 2021, 2:15 p.m.... It's been an honor to work with such a group dedicated to providing the structure and know-how to integrate...example, pd|hub assembled a coalition of higher education organizations committed to advancing career and... inclusive career readiness training in this educational path before we lose more scientists who might... -
More Data for You: Find Articles Citing Addgene Plasmids
TypeBlog PostPublishedJune 16, 2015, 2:15 p.m....'s Addgene ID # has been referenced in other publications, you'll find a link to the list of citing articles...cell-surface programmable DNA-binding domains" publication. If you want to see this new feature in action...lists Benefits to requesting scientists: Quickly locate other papers that have used your plasmid of interest... -
Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies
TypeBlog PostPublishedFeb. 26, 2019, 1:40 p.m....conference, you could be honing your science communications skills and networking. And on social media,...one “safe” project that you know you can get a publication with and one “shoot for the moon” project that... the lab members.”- Jennifer Tsang, Science Communications and Marketing Coordinator Networking “Many,... -
Addgene's Top Blog Posts from 2020
TypeBlog PostPublishedDec. 29, 2020, 2:15 p.m....virtually. 6. Quantifying DNA? Here are Five DNA Quantification Methods to Consider by Jennifer Tsang The...The nanodrop vs. spec debate dominates DNA quantification discussions, but what about the other methods... with plasmids deposited at Addgene! 2. DNA Purification Without a Kit by Beth Kenkel To complement ... -
Let There Be LITE Plasmids
TypeBlog PostPublishedNov. 21, 2013, 3:56 p.m....effectors (TALEs) as anchors for genome engineering applications was still very new. Now, Konermann and her colleagues...prove the causal role of specific epigenetic modifications in the genome,” Zhang said in MIT’s press release...disease states. While LITE plasmids have clear application to anyone with an interest in neuroscience, their... -
Editor's Choice, August 2016
TypeBlog PostPublishedSept. 2, 2016, 1:32 p.m....bit surprising given how many scientists go on vacation in August (for another part of my job, I travel...sequence in live cells. This technique uses catalytically inactive CRISPR endonuclease (dCas9) fused to... need to get back to emailing (hopefully non-vacationing) scientists. You can find all of our August posts... -
Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog PostPublishedMarch 22, 2016, 2:30 p.m.... of 17 signaling pathways that are frequently implicated in cancer cell proliferation, survival, differentiation... make the library usable for pooled screens. Applications of the cancer pathways ORFs kit Wood and his...References 1. Martz, Colin A., et al. "Systematic identification of signaling pathways with potential to confer... -
Management for Scientists: Seeking Feedback
TypeBlog PostPublishedJan. 8, 2015, 2:34 p.m....Scientists series will focus on an aspect of communication especially important for a manager – getting... Creating a culture of excellent, effective communication between all members of a team. As described ... opinions in meetings, via email, at formal communications events or at informal gatherings, like at lunch... -
From our Table to Yours: An Inside Look at Lunch at Addgene
TypeBlog PostPublishedNov. 27, 2013, 2:54 p.m....2 trophies over the years, which is either an indication of the stiff competition or says something about...building activity that is easy to organize. We’ll dedicate a post to these competitions in the future, and...establish, with the goal of fostering better communication between labs. Scientists are often willing to... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog PostPublishedFeb. 18, 2015, 3:09 p.m.... of Visualized Experiments (JOVE). The video publication by Stuart Orkin and Daniel Bauer's labs details... size and frequency [3]. There are several applications in which the production of deletions by paired...useful to study haploinsufficiency. Subsequent modifications at the remaining allele within the deletion ... -
Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies
TypeBlog PostPublishedNov. 27, 2019, 2:03 p.m....experiments! Late last year, we hosted our first AAV Education and Development workshop aimed at brainstorming...even further. For example, injecting AAVs at one location can result in expression of an optogenetic payload...shown in Figure 1, injected retrograde AAVs at one location result in opsin expression in their cell bodies... -
London Calling - Addgene’s New Europe Office in London
TypeBlog PostPublishedJune 10, 2014, 3:20 p.m....Today, Addgene launches a brand new Europe office located in London and operated in collaboration with LGC... their materials. The new office in London is dedicated to helping them find their way to new scientific...newest Addgene Outreach Scientist - the first to be located in London and focused on supporting European scientists... -
Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening
TypeBlog PostPublishedFeb. 20, 2014, 4:57 p.m.... “This enables you to do customized genetic modification on a scale that was really not possible before...genome engineering with CRISPRs was limited to modification of a few loci at a time,” Wang said. “It was... lentiviral libraries and their demonstrated application offer proof-of-principle that CRISPR-Cas9 can... -
SunTag and Fluorescent Imaging
TypeBlog PostPublishedMarch 28, 2017, 2:30 p.m....aggregation, it’s recommended for most imaging applications. What can you do with SunTag? Tanenbaum et al...activation of target genes, but we’ll save that application for another day! References 1. Tanenbaum, Marvin...et al. “A protein-tagging system for signal amplification in gene expression and fluorescence imaging.... -
Five Questions to Ask If You're Deciding Between a Master's or PhD Program
TypeBlog PostPublishedFeb. 16, 2021, 2:15 p.m....technical know-how, and helped develop my science communication skills. Ultimately, my degree prepared me to...technical or product support specialist,” “field application scientist,” or “scientific or technical writer...for PhD applicants since many will also accept applications from master’s candidates with experience. Use... -
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
TypeBlog PostPublishedJune 27, 2018, 1:16 p.m....makes them more adaptable to a broad range of applications. Recently, the Spiess lab developed a set of... with specific genomic loci. He made use of catalytically inactive dCas9 by fusing it to an engineered...only 1 min. Using these lentiviral plasmids, catalytically inactive Cas9 (dSpyCas9) fused to APEX2 is expressed... -
Antibodies 101: Epitope Availability
TypeBlog PostPublishedJan. 11, 2022, 2 p.m....Conformation changes usually fall into one of two categories: ones induced by the researcher and ones that...naturally, by binding to other proteins, changing locations, or even just as part of performing a specific...it a transmembrane protein, or is it know to translocate or fold in certain conditions? It can help to... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog PostPublishedAug. 31, 2023, 1 p.m....channelrhodopsin 2 (ChR2), a blue light sensitive cation channel, fused to mCherry to the brain tissue. ... several months after implantation of fibers, indicating the system was indeed stable for continuous monitoring...interfaces with the entire body, so the obvious next applications are to expand the tool to different brain-organ... -
The Strength of Story Telling
TypeBlog PostPublishedDec. 19, 2017, 2 p.m....Storytelling is a crucial part of interpersonal communication, which makes honing your ability to tell stories...Addgene Blog Check out our blog posts on science communication Find interviews scientists in SciComm careers... science communication...