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We narrowed to 828 results for: cat

Showing: 541 - 560 of 828 results

  1. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...bit surprising given how many scientists go on vacation in August (for another part of my job, I travel...sequence in live cells. This technique uses catalytically inactive CRISPR endonuclease (dCas9) fused to... need to get back to emailing (hopefully non-vacationing) scientists. You can find all of our August posts...

  2. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ... of 17 signaling pathways that are frequently implicated in cancer cell proliferation, survival, differentiation... make the library usable for pooled screens. Applications of the cancer pathways ORFs kit Wood and his...References 1. Martz, Colin A., et al. "Systematic identification of signaling pathways with potential to confer...

  3. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    Published
    Jan. 8, 2015, 2:34 p.m.
    ...Scientists series will focus on an aspect of communication especially important for a manager – getting... Creating a culture of excellent, effective communication between all members of a team. As described ... opinions in meetings, via email, at formal communications events or at informal gatherings, like at lunch...

  4. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    Published
    Nov. 27, 2013, 2:54 p.m.
    ...2 trophies over the years, which is either an indication of the stiff competition or says something about...building activity that is easy to organize. We’ll dedicate a post to these competitions in the future, and...establish, with the goal of fostering better communication between labs. Scientists are often willing to...

  5. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ... of Visualized Experiments (JOVE). The video publication by Stuart Orkin and Daniel Bauer's labs details... size and frequency [3]. There are several applications in which the production of deletions by paired...useful to study haploinsufficiency. Subsequent modifications at the remaining allele within the deletion ...

  6. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type
    Blog Post
    Published
    Nov. 27, 2019, 2:03 p.m.
    ...experiments! Late last year, we hosted our first AAV Education and Development workshop aimed at brainstorming...even further. For example, injecting AAVs at one location can result in expression of an optogenetic payload...shown in Figure 1, injected retrograde AAVs at one location result in opsin expression in their cell bodies...

  7. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    Published
    June 10, 2014, 3:20 p.m.
    ...Today, Addgene launches a brand new Europe office located in London and operated in collaboration with LGC... their materials. The new office in London is dedicated to helping them find their way to new scientific...newest Addgene Outreach Scientist - the first to be located in London and focused on supporting European scientists...

  8. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ...  “This enables you to do customized genetic modification on a scale that was really not possible before...genome engineering with CRISPRs was limited to modification of a few loci at a time,” Wang said. “It was... lentiviral libraries and their demonstrated application offer proof-of-principle that CRISPR-Cas9 can...

  9. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...aggregation, it’s recommended for most imaging applications. What can you do with SunTag? Tanenbaum et al...activation of target genes, but we’ll save that application for another day! References 1. Tanenbaum, Marvin...et al. “A protein-tagging system for signal amplification in gene expression and fluorescence imaging....

  10. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    Published
    Feb. 16, 2021, 2:15 p.m.
    ...technical know-how, and helped develop my science communication skills. Ultimately, my degree prepared me to...technical or product support specialist,” “field application scientist,” or “scientific or technical writer...for PhD applicants since many will also accept applications from master’s candidates with experience.  Use...

  11. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    Published
    June 27, 2018, 1:16 p.m.
    ...makes them more adaptable to a broad range of applications. Recently, the Spiess lab developed a set of... with specific genomic loci. He made use of catalytically inactive dCas9 by fusing it to an engineered...only 1 min. Using these lentiviral plasmids, catalytically inactive Cas9 (dSpyCas9) fused to APEX2 is expressed...

  12. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...Conformation changes usually fall into one of two categories: ones induced by the researcher and ones that...naturally, by binding to other proteins, changing locations, or even just as part of performing a specific...it a transmembrane protein, or is it know to translocate or fold in certain conditions? It can help to...

  13. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...channelrhodopsin 2 (ChR2), a blue light sensitive cation channel, fused to mCherry to the brain tissue. ... several months after implantation of fibers, indicating the system was indeed stable for continuous monitoring...interfaces with the entire body, so the obvious next applications are to expand the tool to different brain-organ...

  14. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...insert comes from using Taq polymerase for the amplification step since Taq polymerase leaves a single deoxyadenosine...rejoining supercoiled DNA ends to facilitate replication. The TOPO technique specifically uses Vaccinia...Addgene.org Find More Plasmid Cloning Protocols Catch Up On Your Basic Molecular Biology Techniques Watch...

  15. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ...Storytelling is a crucial part of interpersonal communication, which makes honing your ability to tell stories...Addgene Blog Check out our blog posts on science communication Find interviews scientists in SciComm careers... science communication...

  16. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...clarify the classification of these cytoplasmic inheritance factors. He proposed the catch-all term “plasmid... as bacterial conjugation and recombination, replication and topology, and cloning and gene expression...

  17. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type
    Blog Post
    Published
    June 4, 2019, 1:02 p.m.
    ...research. This year, we put out another call for applications. We had an overwhelming response and were excited...sensor with proof-of-principle experiments that replicate current knowledge, but the sensor can also kinetically...fluorescent proteins are made. As the bacterial replicate, fluorescent proteins will be diluted out and ...

  18. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...non-trivial, however, as FPs are tremendously complicated entities with a large range of characteristics...decision remains spread across literature in publications that introduce these tools. Years ago, Kurt...incorporate FPbase data in their own projects and applications, a REST API allows programmatic retrieval of...

  19. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...Glutamate plays a variety of roles in synaptic communication and can trigger other forms of neuronal signaling...SF-Venus-iGluSnFR). iGluSnFR3: Improved Glutamate Indicators for Synaptic Imaging However, the first two generations... 30377363 Aggarwal, Abhi, et al. . “Glutamate indicators with improved activation kinetics and localiation...

  20. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...silico approach The in silico approach cannot replicate the complexity of antigen processing events that...This system would somewhat act like a 2-step verification. All approaches have their own limitations. ... together can inform decisions about in vivo application and clinical testing. This would pave the way...
Showing: 541 - 560 of 828 results