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  1. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...dissociation from the GPCR allows for binding of a new inactive heterotrimeric G protein complex and subsequently...pathways from ligand to receptor to G-protein, and finally, to a response? Let’s take a look at a few examples...

  3. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...Ku then acts as a scaffold for recruitment of a kinase (DNA-PKcs) and a two subunit DNA ligase (XRCC4-...reduces breaks and mutations at off-target sites. Christina Mork contributed to the update of this post. Thank...

  4. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ...interested in the same phosphate of your favorite kinase is a good start. Check out Joanne's Reddit AMA ...Networking - Building Relationships for Success" webinar with Joanne Kamens via ASCB Check out more Career...

  5. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...plasmids out of the library in the appropriate combination and do a restriction enzyme digest to cut out...scientists relied on traditional homologous recombination. That technology had been used for decades to...

  6. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ... DNA that you want to assemble together in a combination of different ways according to your cloning strategy... or PCR products from a gel. You will avoid contamination from other DNA fragments and you will remove...

  9. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...expressed. Pho8Δ60 localizes to the cytosol in an inactive form unless autophagy is induced. Then non-selective... Pho8 from its uncleaved to cleaved form is the final read out of this assay. This is also a quantitative...

  10. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...LinkedIn may revoke your ability to send invitations. Finally, if you don’t know clearly why you are inviting...updates, or click bait of any kind. For more on inappropriate content see this article. However, do comment...

  12. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ... (such as LTRs or ITRs), hairpins, or strong terminators. Detailed instructions on other factors to consider...when designing your primers can be found here. Finally, you will want to have a well-organized database...

  16. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...carrying through on the actions you committed to. Finally, hiring a paid coach will make you take the experience...sensitive to this and have coaching plans that are financially accessible. Sometimes only a few hours of coaching...

  17. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...Originally published May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology...immune system of bacteria. These Acr proteins were originally found in type I CRISPR-Cas systems.   Four years...

  19. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...conjugated to the enzyme horseradish peroxidase (HRP). Finally, the substrate of HRP is added to the wells. This... the amount of your protein of interest in the original sample. The competitive ELISA is best used for...

  20. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ... DNA, an editor targets DNA (via catalytically inactive dCas9) to modify chromatin. A fluorescent protein...Ward lab created a tool-kit to expand upon these original tools that allows for both spatiotemporal protein...
Showing: 541 - 560 of 764 results