... protein abundance. Finally, some degron tags can do more than just degrade! Read on to find out more....

...01756-1. PMID: 37095348. Breaking up is hard to do: Monomeric StayGold variants By Mike Lacy In 2022... it with an “assist peptide” that facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA...based vectors (and vice versa), enabling repeat dosing. In a proof-of-concept study, mouse LY6A was delivered...

...electrical charges propagated through multiple cells to do things like translate perceived signals to physical...

...ancestral groups or, by extension, race. Many researchers do large-scale CRISPR screens in cancer cell lines, ...

...Cre-expressing neurons in a transgenic mouse line. By doing this, RABV expressing the viral protein EnvA on ...

...views in this post are those of the guest blogger and do not necessarily represent the views of Addgene. Addgene...

...Additional protein tagging systems For those who do not work with C. elegans, several groups have designed...

...it is essential to make sure that the selected FPs do not influence each other and thus potentially falsify...

...Integrative and conjugative elements (ICEs): what they do and how they work." Annual review of genetics 49 ...

...more about xCas9 - here’s what we know so far! Why do we need PAM flexibility? IDT estimates that there...

...microbes to visualize host/pathogen interactions.   Do you have a favorite way to use GFP?  Comment and ...

...replication-competent lentivirus (RCL) in your target cells.  Q4: How do the packaging systems differ between 2nd- and 3rd-generation...

..., Freund E, Ricci MS, Carpenter JF, Lengsfeld CS. Do not drop: mechanical shock in vials causes cavitation....27776. Epub 2021 May 4. PMID: 33811659. Pace CN, Vajdos F, Fee L, Grimsley G, Gray T. How to measure and...

...ddPCR for AAV quantitation blog post.  We generally do not recommend using ELISA-based titering assays, ...determination of the median tissue culture infective dose (TCID50) (Zen et al., 2004).  To choose the optimal...

...evaluate tens of thousands of designs in parallel. To do this, we performed high-throughput ‘self-targeting...

...high-quality data for our DNA-based reagents. Not only do we extract as much information as possible from depositing...

...your research, be sure to consider how its features do and don’t support reproducibility as not all ELNs...

...Agrobacterium strains see Weigel and Glazebrook, 2002. Tips: Do a colony PCR on your agrobacterium: Getting a transformant...

...single molecules - something researchers often want to do at neuronal synapses for instance. Also these tags...

...systemic delivery of high doses of vectors are needed. Additionally, AAVs do not integrate in the host...