...give rise to a starfish shape. You can ask me about the different arms of this starfish sometime, but... plug-in called Maps.  Figure 1: See a starfish? This is actually a map of Joanne Kamen's LinkedIn... it is completely impossible for me or anyone to stay in touch with all of their connections on a regular...

...different water filtration system, environmental stability in an incubator), but there’s a lot you can do...other researchers to help them troubleshoot and understand. All of this communication and sharing fits together...seriously. That’s why at Cell Press we developed STAR Methods to improve the structure and transparency... of checklists and guidelines to help authors understand what they are expected to do. All of that helps...

...is fine for its goal of creating a choice of standardized constructs and tools for the rational and simple...Golden Gate Assembly also allows the creation of standardized DNA constructs, but its use of Type IIS restriction...polymerase used in the amplification. This tool allows instant updates as fragments are added, deleted, or reordered... in biofuel generation, green chemistry and understanding the minimal genome. Many of these techniques...

...efficiency of these editing reactions is low. Understanding the biological cause of this repair bias is ... reinforce a key scientific principle: that understanding a biological process in detail often suggests... efficiency of sequence replacement by HDR. Understanding Cas9 biophysics enhances HDR We first measured...released strand, 127bp in length, With 36bp on the PAM-distal side of the break and 91bp on the PAM-proximal ...

... the purpose of the meeting: Begin meetings by stating “The purpose of this meeting is to _________." ... what you need to accomplish to get to the next stage, and then adjourn. Define the tone of the meeting...template for project meetings. Team Meetings: For standing team meetings, provide agenda sections for each...meeting. Accountability: At your next meeting, start by checking in on the action items from last meeting...

...Imaging Improving Well-established High-throughput assays Improving Well-established Biological sources...cells. Luciferase assays have since become a gold standard in gene expression analysis and a luciferase gene...Phrixothrix hirtus), and a variety of marine crustaceans. Many of these luciferases have been subjected...

...This method utilizes a packaging cell line that stably expresses Gag-Pol and/or Env, eliminating the need...packaging cell line developed by Garry Nolan at Stanford (Swift, 2001) contains Gag-Pol and either an ecotropic...differentiated (non-dividing) cells. Both lentiviruses and standard γ-retroviruses use the Gag, Pol, and Env genes...provirus. 3’ LTR Terminates transcription started by 5' LTR by the addition of a poly A tract just...

...at (and through) a couple of models. Before you start a new project with fluorescent proteins, the best...this series of posts are (i) brightness, (ii) photostability and (iii) aggregation tendency. Brightness...Without further information about properties like photostability or aggregation tendency, our choice would be...Goedhard and Marieke Mastop! Joachim Goedhart is an assistant professor at the Section of Molecular Cytology...

...promoters. Others are only active under specific circumstances. In this post, we’ll discuss inducible promoters...promoters, which can be switched from an OFF to an ON state, and how you might use these in your research. When...negative control. Positive inducible In the OFF state, the promoter is inactive because the activator ...transcription.     Negative inducible In the OFF state, the promoter is inactive because a bound repressor...

...phenotypes than standard RNAi protocols without the need for mutant genetic backgrounds. The standard RNAi vector...biochemical and biophysical study involving protein stability or binding experiments. Potential experiments ...perform using the purified domains include protein stability, small molecule binding screens, peptide arrays...

...infect cells and deliver cargo, it’s important to understand their differences to choose the best one for ...viruses Retroviruses are a family of viruses that stably integrate their genome into the host genome, setting...   Type of Virus Pros Cons Retrovirus Stable Gene Expression Broad Tropism Only Infects ...virus safety and viral applications to help you start (safely!) thinking about the possibilities. Ready...

...reaction to understand if any of your buffer components could affect the reaction. For instance, sodium azide...either testing the antibody efficacy through a standard curve, another quick experiment, or using a different... to use, not sensitive to light in its inactive state, and cheap. Unless there’s a specific reason you...

...for colonies that contain your plasmid is a great start when cloning, but how about being able to choose..., and remain the lovely whitish-cream color of standard E. coli. On the other hand, intact β-galactosidase...colonies that not only have your plasmid (antibiotic resistance), but also confirm those plasmids have your insert...plasmid-containing cells without using antibiotic resistance. These rely on cell lines susceptible to or dependent...

...weeks is the standard ballpark estimation. Once expressed, viral cargo is usually quite stable and in some...and promoter) for use in your experiment! As a starting point, we recommend checking the literature and...272. PubMed PMID: 12349826. Xiong, Wenjun, and Constance Cepko. "Distinct expression patterns of AAV8 vectors...

...protein degradation due to other pathways, it is standard practice to compare degradation rates between ...identify because their contents can be at various stages of degradation. Additionally, electron microscopy...that affect autophagy (ATG) in yeast. Atg8p (“p” stands for protein) associates with autophagosomal membranes...green fluorescence. After 4 hours of nitrogen starvation to induce autophagy, red but not green fluorescence...

...ratio, high sensitivity, responds quickly, is photostable, and is compatible with optogenetic control. ...proteins that bind to either the light- and dark- states of the photoswitchable cPYP, which changes in conformation... partners that bind to only the light- or dark- state of the photoswitchable proteins offers a powerful...binders (which bind to either the light- or dark- state of the photoswitchable proteins) fused to mVenus...

...interfere with fused protein function Under some circumstances, the fused fluorescent proteins can be a roadblock...that some fluorescent proteins can be, in some circumstances, detrimental to the expression of the protein...be beneficial in some types of experiments (for instance, when the fluorescence expressed in the neuropil...techniques, her research aims to gain a deeper understanding of the physiology and pathology of the basal...

...the problem and gather information to help you understand it. Step Two: Come up with and evaluate potential...You might do some background research to better understand it and come up with several hypotheses on the...problem solving process on your own, but you can start by paying attention to how your mentors reach solutions...to remember that you have these skills when you start applying and interviewing for new positions. Highlighting...

...will enable significant steps forward in our understanding of synaptic and circuit functions in health ...metabolites are vital for researchers to better understand the processes that happen inside the cell,” says...Together, this project will allow us to better understand how neuron activity changes following opioid ...improved voltage indicator coming from the screen to understand mouse cortical and hippocampal neural microcircuits...

... of replication, protein tags, and antibiotic resistance markers (just to name a few). In this edition... I use Cre-lox? The Cre/lox system is a well-established research tool, especially in the field of mouse...targeting, targeted clones are selected for using a resistance marker; however, it is often desirable to remove...in certain cell or tissue types, during certain stages of development, or by making the Cre inducible ...