... to make a plasmid. Doench’s lab in the Broad Institute has created hundreds of thousands of plasmids,...also a great way to get to know people doing interesting and relevant work in your field! However, there...my ability to tell. But even from these rough estimates, it’s clear that community engagement with and...

...tet-off turns off constitutively expressed proteins while tet-on turns on constitutively silenced proteins...know what the job requirements are. Here are the questions you should be asking. What kind of control? On...

...- These fragments are produced by proteolytic digestion that retains an antibody’s disulfide bonds within...resulting product is a mini-Y with both Fab regions still attached to one another. Size: ~110 kDa. Fab' - ...- These fragments are produced by proteolytic digestion above the hinge-region, which results in two independent...

...initiates single stranded DNA degradation. Cas3 DNA digestion can proceed up to multiple kilobases, but only...that it generates staggered breaks, also known as sticky ends. Cas12a cleavage results in 5 bp overhangs...efficient, multiplexable RNA knockdown. Cas13 has also stirred up interest for its potential as a viral therapeutic...

... admittedly, somewhat meaningless) practice of listing “Submitted” articles in their CV or grant applications...directly alongside the preprint while the work is still in progress or awaiting a final publication in a...Motivations, concerns and selection biases when posting preprints: a survey of bioRxiv authors. bioRxiv...

...this though) about the fact that containers are distinct from their contents. You should be tracking both...tray or a freezer. Some are disposable, like a plastic tube. Talk with your lab team about how they would...getting up and running and more time working on interesting biotechnology code.There are valid technical ...

...one-pot reactions with straightforward restriction digestion and ligation steps to build barcoded lentiviral...pool of lentiviral plasmids. Multiple rounds of digestion and ligation result in plasmid libraries containing...tailored to address the users’ specific research questions. CombiGEM has been successfully applied to functionally...

...highlighted new tools but also included time for questions and a group discussion on the best strategies ... the Arizona State University (ASU) Biodesign institute developed a 3’UTR database specifically for C.... to activation sequences upstream of genes to stimulate expression (Wang et al., 2016). In 2018, the lab...

...include Swiss Webster and Harlan (Hsd) National Institutes of Health (NIH) Swiss. Genetically engineered...injection of a targeting vector alone, some challenges still exist. Nucleases may cause off-target effects on...the transgene in specific cells or under certain stimuli. Cre recombinase catalyzes the recombination of...

...and 5 lentiviral Destination vectors. These vectors can induce combinatorial constitutive or inducible gene...produce their shRNA library to support functional investigations of the mammalian genome that can reveal how...

...you can make big changes in your career path and still find comfort and happiness there. Read all of the...part of why they work at Addgene. I posed this question to all nine of my interviewees. Fabienne Furt,...people.” My absolute favorite response to this question came from Lucy Hammer, our Financial Operations...

...option in early 2020 and eventually replace the existing version of PubMed.  Key features This new version... for getting a quick summary of papers before investing the time to read them. Organizations: Accounts...AI-backed literature search developed by the Allen Institute for Artificial Intelligence. Semantic Scholar ...

... be used for the development of vaccines and diagnostics (Hsieh et al., 2020). As the virus spreads around...also deposited plasmids for producing COVID-19 diagnostic enzymes. Plasmids in this toolkit include ones...starting point for SARS-CoV-2 researchers to investigate how the virus interacts with human cells. Addgene...

...to keep track of growing collections of music. I still have a shelf full of CDs that have been gathering...Therefore, researchers from over 100 scientific institutions have proposed a shift to recombinant DNA-based...enormous amount of wasted money and time, with an estimated $350M/year lost on low quality antibodies in the...

...Repair of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem ...adult stem cells isolated from two patients with cystic fibrosis (Schwank et al). They were able to demonstrate...

... instead that it is noninvasive and has longer-lasting effects. Building your chemogenetic system To set...they retain their intracellular interactions and still set off the same pathways, giving rise to the same... create ultrapotent PSEMS (uPSEMs) that have drastically increased potency. Download our chemogenetics...

...of expertise, equipment, or excellence of the institution). Likewise, if you’re staying in the same lab... applying for smaller grants from within your institute or a private funding body. Success breeds success...proof-of-principal study were very helpful in assisting me with applications for much larger fellowships...

...You’ve done great research and made interesting discoveries. You’ve analyzed the data and generated beautiful...should be easily navigable and efficient; your questions, data, and conclusions should be clearly identified...spacing between them so that they are easily distinguished. The UCLA Library offers some Powerpoint based...

...paternal grandmothers who experienced famine while still in their mother's womb passed on a trait to their...grandchildren; in this study,epigenetic changes were statistically significantly associated with reduced life-span...hybridization, methylation-sensitive restriction enzyme digestion, DNA adenine methyltransferase identification,...

... Khan Khilji, a PhD student at the Max Planck Institute of Colloids and Interfaces. Here are some tips...wiped with 70% ethanol immediately. Finally, avoid sticking pipettes into solution bottles directly, rather...soon be joining her PhD lab at the Max Planck Institute of Colloids and Interfaces. Sana is particularly...