...References: Monticelli, M., Ferro, T., Jaeken, J., dos Reis Ferreira, V. & Videira, P. A. Immunological ...

...colony of genetically engineered mice, what can you do? In this post, we’ll cover why and how to cross mice...

...: A New Tool for CRISPR Genome Editing Which Cas9 Do I Choose for My CRISPR Experiment A Match Made in...

...off"). These Cre-off switches may be referred to as DO (Double-floxed Orientation) and could be used to ...

...Writing Scientific Manuscripts by Johnna Roose Dos & Don'ts When Publishing a Scientific Manuscript ...

...application process? What types of writing feedback do you find most helpful? Have a great grant writing...

...Doench, “Whole plasmid sequencing is just part of doing good science!” Find the Fragmid kit here!  Cloning...

...backbone. Purpose Answer “What does this plasmid do?” in <200 characters. A descriptive purpose focuses...

... provided you with a few ideas to consider if you do run into a roadblock and encourage you to check out...overcome this issue, we recommend using a range of doses of lentivirus when transducing a target cell line...transduction experiment. Media choice, reaction vessel, dose of virus, volume and timing of transduction will...

...Despite their advantages, mammalian expressions systems do require demanding cell culture conditions compared...

...after knockdown of beta-catenin. Other gene targets do not require amputation to be visible. For example...

...  Listen to the CRY2clust podcast segment The Won Do Heo lab at the Korea Advanced Institute of Science...

...been used to treat 10 infants with this disease. To do so, their T-cells were grown in vitro, their mutations...to be untreatable until 2008. Following a single dose of subretinal administration of rAAV2 carrying the...

...every C.elegans  neuron. Scientists are currently doing this using two approaches: single-cell RNA-sequencing...

...blurb you will use to introduce yourself and what you do. You might want to include your university and your...

...Addgene.org Find Info on How to Quantify Your DNA Learn to Do a Bacterial Transformation Learn How to Perform a...

...study of heritable changes in gene expression that do not involve any changes to DNA sequence (Epigenetics...

...plasmid tools that have come through our doors in 2015. Do you have a favorite new plasmid tool from the past... are turned on or off in a given area and the can do so in a very specific, precise manner. Previously... are bound to the coding region are displaced and do not rebind because they are transported back to the...their quest to understand lncRNAs: What can lncRNAs do by themselves? The Rinn lab has developed a novel...regulation has roles in many cellular functions, from endosomal trafficking to signaling pathways to the cell ...reticulum (ER), mitochondria, cytoskeleton, and endosomes. These fusion proteins can act as markers for ...Microtubules mCherry-alpha-tubulin Early Endosome Vacuolar Compartment mCherry-Rab5 BFP-Rab5 ...

... used the library to identify RBD mutations which do not interfere with ACE2 binding, but impact recognition...

... protein abundance. Finally, some degron tags can do more than just degrade! Read on to find out more....