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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 628 results for: Dos

Showing: 501 - 520 of 628 results

  1. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...sequences to visually check for homology and mismatches. Do you have your own best sequencing practices or maybe...

  3. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ... ends better substrates for ligation. Although we do not describe these steps here, the processing of ...

  4. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...Have you used the Cre-lox system in your research? Do you have any tips or tricks, or clever cre-dependent...

  6. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...invaluable resource. I really appreciate the work you do to make sharing reagents and protocols so easy!” ...

  11. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...diagnosis (PGD) screens embryos to find those that do not carry a given dominant disease-causing gene, ...

  12. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ... unconjugated version, but if you find yourself doing your own conjugation, it is important to keep in...

  13. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...resources on the Addgene Blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? The PAM requirement...

  15. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...off"). These Cre-off switches may be referred to as DO (Double-floxed Orientation) and could be used to ...

  19. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    .... Fun fact! Some older plasmids in our collection do not have a full sequence, as they were deposited ...

  20. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...Doench, “Whole plasmid sequencing is just part of doing good science!” Find the Fragmid kit here!  Cloning...
Showing: 501 - 520 of 628 results