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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 622 results for: Dos

Showing: 501 - 520 of 622 results

  1. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ... ends better substrates for ligation. Although we do not describe these steps here, the processing of ...

  2. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...Have you used the Cre-lox system in your research? Do you have any tips or tricks, or clever cre-dependent...

  3. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...invaluable resource. I really appreciate the work you do to make sharing reagents and protocols so easy!” ...

  7. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...resources on the Addgene Blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? The PAM requirement...

  8. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ... unconjugated version, but if you find yourself doing your own conjugation, it is important to keep in...

  10. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...diagnosis (PGD) screens embryos to find those that do not carry a given dominant disease-causing gene, ...

  12. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...off"). These Cre-off switches may be referred to as DO (Double-floxed Orientation) and could be used to ...

  16. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    .... Fun fact! Some older plasmids in our collection do not have a full sequence, as they were deposited ...

  17. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...Doench, “Whole plasmid sequencing is just part of doing good science!” Find the Fragmid kit here!  Cloning...

  20. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ... provided you with a few ideas to consider if you do run into a roadblock and encourage you to check out...overcome this issue, we recommend using a range of doses of lentivirus when transducing a target cell line...transduction experiment. Media choice, reaction vessel, dose of virus, volume and timing of transduction will...
Showing: 501 - 520 of 622 results