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We narrowed to 788 results for: lat

Showing: 481 - 500 of 788 results

  1. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ...launched this pilot in 2019 as an open science platform for scientists using Addgene AAV viral preps to...all of your data prepared. All of these questions relate to factors that you should have already recorded...reproducible research. Share your data on AAV data hub! Related resources on the Addgene blog Important Considerations...

  2. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ... Pre-warming your antibiotic-containing plate prior to plating your transformation may allow you to see...TOPO technique specifically uses Vaccinia virus-isolated topoisomerase I as this enzyme recognizes the ...

  3. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...protein could cleave DNA in vitro, directed by phosphorylated DNA guides. Unfortunately, Swarts et al. were...able to mediate homology-directed repair when a template is supplied. Find Plasmids from the NgAgo Publication...in GC-rich regions. DNA guides NgAgo uses 5’ phosphorylated DNA guides (so called “gDNAs”) rather than ...

  4. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...plasmid” derived as a hybrid of "cytoplasm" and "id" (Latin for 'it'), as “a generic term for any extrachromsomal...integrated into the chromosome” was proposed a few years later by Élie Jacob and François Wollman and became the...PMCID: PMC427208. Novick, R P et al. “Uniform Nomenclature for Bacterial Plasmids: A Proposal.”Bacteriological...

  5. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...repair template plasmid to create fluorescent and 3xFLAG-tag fusion proteins. The repair template and Cas9...worms have laid eggs, hygromycin is added to the plates to select for candidate knock-ins. These candidate...

  6. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ... Primary cells recapitulate the natural biology of a cell type of interest better than immortalized lines...to describe this method of protein transduction. Later studies showed that iTOP functions through macropinocytosis... sorted into single wells to generate clonal populations. After iTOP treatment, both primary (hESCs) and...

  7. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...targeting the genes responsible for both early onset and late onset AD. In this study, the group showed that CRISPR...Alzheimer’s disease, as well as those that play into late onset AD (5).     CRISPR/Cas9 corrects Alzheimer...CRISPR to disease correction in adult stem cells isolated from two patients with cystic fibrosis. They were...

  8. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...expensive) the different machines are, and how many regulations are in place to keep everyone safe. None of this...fluorescent protein through transcription and translation. Unfortunately, having live cells in a classroom...hands-on educational activities using the BioBits platform, please get in touch with us! We’ll work with ...

  9. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...at Addgene began working from home last week to flatten the curve, but working remotely can be more difficult...this period, check out these tips and resources collated by Addgenies to help you keep calm and science...local businesses (ex: purchasing a gift card to use later or ordering take-out) or volunteering to foster ...

  10. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    Published
    July 27, 2017, 1:11 p.m.
    ...ways to apply fluorescent proteins everyday.  The late Michael Davidson and Roger Tsien played enormous...enzyme active site and shutting off the enzyme. Later exposure to cyan light stopped Dronpa oligomerization...Fluorescent Light-inducible Proteins, or FLiPs.  Xin later optimized these first generation FLiPs by mutating...

  11. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...communicate outcomes to the rest of their team. Here's a template for project meetings. Team Meetings: For standing...members still have their voices heard. Here's a template for team meetings. One-on-Ones: Managers should... agenda template...

  12. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...people choose a reversed chronological style (the latest to the earliest) or a functional one. Pick one ...when the research topic you have done is closely related to the job you are applying for, in which case ...in Experimental Medicine. She is the Community Relations Director for Curiosity Collider (hyperlink https...

  13. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...be covering this ever-versatile fluorophore in a later post, so stay tuned! Transient transfection and ...transfection A stable transfection is used to create a population of cells that have fully and successfully incorporated... COS-1, 293HEK Inhibits termination step of translation 2-10 ug/mL G418/Geneticin neo HeLa, NIH3T3,...

  14. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...cellular extracts to perform transcription and translation in vitro, and the new BioBuilder module allows... mentors, and provides a nationally recognized platform in March to exhibit projects at any stage of completion...breakdown, production of fire-resistant housing insulation, and cleaning up salt runoff from winter icing.BioBuilderClub...

  15. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...plasmids for expression of mAbs, the Trimmer lab isolated RNA from cryopreserved hybridomas, immortalized...then used to generate cDNA, which  was used as a template for PCR amplification with a degenerate primer...grown in tissue culture, they were also able to isolate RNA from non-recoverable hybridoma stocks, without...

  16. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...miniature CRISPR-Cas system for mammalian genome regulation and editing Anti-FLAG Frankenbodies for modular... genes, researchers at the Genetic Pertubation Platform at the Broad Institute have expanded the genetic...miniature CRISPR-Cas system for mammalian genome regulation and editing by: Rachel Leeson The large size ...

  17. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...hepatitis virus post‐transcriptional regulatory element; sequence that stimulates the expression of transgenes...-retroviral plasmids have similar names? The nomenclature of the plasmid backbone is meant to be descriptive...

  18. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    Published
    Jan. 21, 2025, 2:15 p.m.
    ...lab or pivot to a managerial role. For those contemplating the leap into management, the journey can be...being a great scientist doesn’t automatically translate to being an effective manager. Mismanaging (no... Kim Scott Courses: Leadership programs on platforms like Coursera, LinkedIn Learning, or edX. Podcasts...

  19. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...sequence (NGG, etc.) as well as their orientation relative to the gRNA (5’ or 3’). In some cases, there is... used by Jinek et al. 2012, or browsing for the latest AsCas12a plasmids, Addgene and CasPEDIA have got...to browse entries by the current phylogenetic nomenclature and discover orthologs with similar properties...

  20. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...scientific and medical tools. As scientists, we can manipulate what this cargo is and where it goes, turning...mutagenesis in the host. Adenoviruses can deliver relatively large cargo inserts (>8 kb). Adeno-associated...their low immunogenicity, which means they do not stimulate a strong immune response when introduced into ...
Showing: 481 - 500 of 788 results