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Showing: 461 - 480 of 680 results

  1. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...moments. The models function as thinking tools – eliciting questions like “what does this model representt...or trying to explain your science to the general public, visit the Center for BioMolecular Modeling web...

  2. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...to amplify the sample for further downstream applications. As individual libraries can vary significantly...containing smaller, recombined backbones that may replicate faster. For this reason, if your library has inserts...

  3. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ... into Addgene’s CRISPR data? Read our recent publication, “The Expanding Dissemination and Distribution...support for, this and previous Addgene CRISPR publications.  References and resources References Pyhtila...

  4. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ..., and making sure we always maintain a backup replicates of each clone in case of contamination. We do...population of transfected clones. The technique is applicable to a wide variety of cell lines, from HEK to ...

  5. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ... membranes is tricky because they have fewer hydrophilic surfaces, require detergents or lipids to remain...2020) Generation of synthetic nanobodies against delicate proteins. Nature Protocols 15:1707–1741. https...

  6. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ..., blastp, and blastx searches! Now, before you click the BLAST button, consider the Program Selection...may have noticed the “Algorithm parameters” link. Click on this link to view advanced BLAST options and ...

  8. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...muscular atrophy, neurodegenerative diseases and metabolic diseases are also underway and reported results...-1250015778 7. Yu W and Wu Z., 2018. In vivo Applications of CRISPR-Based Genome Editing in the Retina...

  9. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...are often associated with rote memorization of metabolic pathways (precisely the type of exercise Einstein...mitochondrial MDH (hMDH2) with those from human cytosolic MDH (hMDH1). They then expressed the original ...

  10. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...missing so that we can make a new tube from a replicate sample. The transformation After the DNA has ...plasmid can grow. As the bacteria divide, they replicate and retain the plasmid. This allows us to make...

  11. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...protein evolution By Joanne Kamens Mateo Sanchez and Alice Ting have used one biotechnology tool to optimize...cultured and FACS sorted. Image from Mateo Sanchez and Alice Ting. Sanchez MI et al., Nat methods. 2019. https...

  12. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...community is no small task. The EDGE program solicitation requires funded labs to rapidly disseminate ...dissemination of information and materials. Many groups publicized workshops on their organismal systems for scientists...

  14. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...involves a few key features that can be difficult to replicate in other work settings: The sense of being the... scientist position at Addgene, the skeptical applicant asked if she would partake in “critical thinking...

  15. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ... vehicle for making research data available for public access, review, and query. Gates Open Research ... both accept deposit of data associated with publications into one of five general purpose open source...

  16. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...instances, variations of the product’s specific application instructions or “optimized steps” can be linked...customers may tell all sorts of details about the application, just not the ones that matter. Therefore, if...

  17. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ... the bacteria were injected, limiting their applicability. They also did not scatter ultrasound nonlinearly...promoter pBAD and a low-copy number origin of replication (p15A) worked best under a wide range of inducer...

  18. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ... from depositing laboratories and associated publications, but our quality control (QC) standards have...can be easily shared with others without any complicated setup. We created, tested, and debugged each ...

  19. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...be part of the answer. Problems with written publications Online tools like ResearchGate, GoogleScholar...Freedom: The Expansion of Data Sharing in Research Publications Resources at Addgene.org Check out our Protocol...
Showing: 461 - 480 of 680 results