Skip to main content
Addgene
Showing: 461 - 480 of 604 results

  5. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...could also be a tool for lineage tracing cells that do not tolerate double-strand DNA breaks by introducing... the culture, so bacteria must be grown in a turbidostat. Only mutate particular types of nucleotides...

  6. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ... their low leakiness. Inducibility level How much do you want to induce transcription of your target gene...

  7. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...students are asking. Teachers are also asking, “How do I engage my students in real-world lab activities...

  9. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...about the types of experiments you're planning on doing and read the eBook or browse our CRISPR resources...

  11. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...) antigens. Unfortunately, antibodies frequently do not work as assumed (Ayoubi et al., 2023). Additionally...may not be available to every scientist looking to do a flow cytometry experiment. But validation is critical...

  12. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...domains—including the CTD and the entire REC lobe—do not structurally resemble any other known proteins...check for sequence matches; a perfect guide RNA will do you no good if there is no adjacent PAM to anchor...

  13. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...strength of staining and background noise. If you do use different lots of your polyclonal antibody, you...

  16. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...produced dsRNA rarely reach this threshold and thus do not typically activate an inflammatory response. ...

  17. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...broader community, and preprints are a great way to do that. We are happy to receive deposits of plasmids...

  18. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...lentiviral inventory is based on CRISPR tools, though we do provide a few non-CRISPR related viral preps. Cas9...
Showing: 461 - 480 of 604 results