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Showing: 421 - 440 of 689 results

  1. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...chromoprotein detection can be done by the naked eye. This makes cloning quite a bit easier since you can see if ...Learn more about FRET Find protocols and tips for making plasmid constructs Resources on Addgene.org Find...

  3. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ... inadequate plasmid DNA yields. Why would volume make a difference? In the simplest of terms, more volume...cells present in the given volume.  Let oxygen in! Make sure your culture is getting the right amount of...

  5. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ... learned along the way and hope they’ll help you make the most of the opportunities you’re provided throughout...erlenmeyer flask with gold glitter spray paint to make a trophy for the winner. We promoted the contest...

  6. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...developments in microscopy coupled with techniques that make tissues transparent are enabling microscopes to ...point-by-point scanning along the entire specimen makes imaging too slow and unwieldy for large specimens...

  7. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...accurate, as these peptides are thought to function by making the ribosome skip the synthesis of a peptide bond...within the same multicistronic vector, effectively making a construct that expresses multiple unique genes...

  8. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...or validating your new clones, try these tips to make the most of your time and sequence! You might also...Standard Nucleotide BLAST page, the first decision to make is whether to compare a Sanger sequencing result...

  9. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...are of no use to you. When using more than one FP, make sure their emission light does not overlap in wavelength...eel was shown to mature independently of oxygen, making suitble for use in anaerobic conditions. Maturation...

  10. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...has greatly enhanced the ability of scientists to make genomic alterations, bringing about a revolution...the cell via protein degradation pathways. This makes Cas9 RNPs useful for CRISPR applications where limited...

  11. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ... a specific field. While searching for jobs and making connections, avoid falling into an identity trap...yourself, and to demonstrate them to the people who make hiring decisions. Though having all the technical...

  12. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...Multi-color labeling of neurons is for more than making pretty pictures (see figure 4). It also enables...a specific cell type in the brain, you needed to make a transgenic mouse. Combining AAv.PHP.eB’s or AAV.PHP.S...

  13. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...powder slurry that has a milk-like appearance. To make DNA stick to the glass particles in glass milk, ...but the Zhao and Deng labs found they could just make their own buffers when the kits buffers ran out....

  14. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...our samples 1:6 million to 1:25 million.  After making the dilutions, they are transferred to a new plate...generator, you should avoid pipetting too quickly when making your virus dilutions. This will reduce aerosols...

  15. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...tumors. Lentiviral vectors are also commonly used to make stable transgene-expressing or knockout cell lines...clinical stages. Researchers are also working to make lentiviral vectors safer. Since lentiviral vectors...

  16. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...resources are being spent to make something the cell doesn’t need to make. Eukaryotes and some bacteria...

  17. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...excited because genome-editing with these molecules makes it much easier to knock-in LexA binding elements...developing several additional systems of enChIP to make the technology more user-friendly and precise. We...

  18. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...buffer components can interfere with conjugates. Make sure to do your research before conjugating at the...The assay only uses one antigen binding antibody, making it less specific than other methods. In addition...

  19. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...effective and with no additional off-target edits, making them ideal for size-limited AAV delivery. PE6c ...and MMLV gag, to specific cell types. It can also make CAR-T cell therapy more accessible by simplifying...

  20. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...as well as high spatial and temporal resolution, making it ideal for visualizing gene expression in vivo...They also did not scatter ultrasound nonlinearly, making them difficult to distinguish from background tissue...
Showing: 421 - 440 of 689 results