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We narrowed to 787 results for: lat

Showing: 401 - 420 of 787 results

  1. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...of interest (4) isolated your antibody:protein:DNA complexes and finally (5) isolated the DNA from your...DNA are no longer bound, and the DNA can then be isolated and studied through a variety of downstream methods...downstream analysis via any methods suitable for isolated and purified DNA, such as sequencing or qPCR. ...

  2. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ... to glance at as you work. Draw your plate maps onto your plates, alongside your physical copy. If you...reminders on a sticky note   Visual Planning  Plate maps, pre-labeling tubes, and even drawing out experimental...example, setting up your pipette tips to match your plate layout can help you track samples as you add them...

  3. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...multiplexing vectors are larger because they require regulatory sequences to allow for expression of multiple...example of how this works. Make sure to order 5’ phosphorylated oligos or treat with T4 PNK. Delivery options... are easier to use across multiple expression platforms: transfection, lentiviral transduction, and AAV...Multiplex gene editing with CRISPR-Cpf1 is one of the latest developments in CRISPR technologies.  It’s a simple...

  4. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...participants in skills useful for future mentoring relationships.   If you would like to start a group mentoring...for the time commitment of a formal mentoring relationship or there aren’t enough senior mentors available...ideas on what to do once you are in a mentoring relationship.  Celebrate your successes and support each... come. Are you involved in Peer Mentoring relationships? Comment below with ideas from your own mentoring...

  5. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...The modularity of antibodies means that it is relatively easy to physically divide up the different regions...you to use just a fragment of the antibody in isolation from another portion. For example, Fab fragments...but not the Fc region (more on when that might be later). Find scFv plasmids at Addgene Depending on how...fragments intended for use as primary antibodies are relatively uncommon. Though, if you want to use Fab fragments...

  6. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...mechanism? Where is the control happening? Is the regulation of an exogenously introduced gene or over the...generate a frameshift mutation that will permanently ablate protein expression. If you need the ability to...different amounts of tetracycline. Tetracycline regulation is reversible (when the drug is removed expression... These control systems function at the post-translational level, unlike most of the other mechanisms described...

  7. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ... with post translational modifications. One of the most common of these is 2’-O-methylation, where a methyl...once you’ve chosen a gRNA and modification(s) Congratulations! You’ve picked your perfect guide; now check...Spatiotemporally Controlled CRSIPR-Cas-based Genomic Regulation. ACS 6, 5, 695-703 (2020). https://pubs.acs.org...

  8. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ... TAK 1, an enzyme that controls apoptosis and regulates transcription. Without TKA1, cells proliferate...cancer as a broad phenomenon and not only as an isolated disease. While the tumor may seem to take center...microRNA transfer: A mechanism of environmental modulation of hepatocellular cancer cell growth." Hepatology...resources on the Addgene blog Read our cancer related blog posts Learn about our cancer collection pages...

  9. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ... not linearly related to power, the conclusions reached at high power may not translate to real applications...widefield imaging fluorophores are excited with relatively low light levels (for a prolonged time). Other...application (Goedhart et al, 2012). The third issue relates to the environment of the fluorescent protein. ...

  10. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ... that underlie complex regulation in biological systems. The CombiGEM platform provides a means to create.... CombiGEM-CRISPR: The marriage of two simple platforms CRISPR has revolutionized how we decode the genome...Wong et al., 2016). With CombiGEM and CRISPR platforms now being integrated, we look forward to the realization...

  11. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...samples through our lab using barcoded tubes and plates. The critical pieces of information for a given...100 µl of glycerol stock to each of 2 96-well microplates (identical copies, see image above). We then ... database of over 2000 primers. Two 96-well PCR plates are prepared with the primer stocks for forward...shipped along with their corresponding glycerol plate on dry ice to the sequencing provider. Each sequencing...

  12. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...glycosyltransferase genes and other proteins related to cellular glycosylation. Get the library at Addgene CasRx...requires the much less efficient double stranded templates, rather than the more efficient single-stranded...cellular protein. They used CRISPR with a ssODN template to introduce the small wrmScarlet11 which is less...

  13. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    Published
    May 22, 2018, 1:38 p.m.
    ...acquired divergent functions due to mutations accumulated over time? Undergraduates can help to answer ...If the phenotype is corrected, students can be relatively confident that the gene from the test species...the S. cerevisiae met deletion strains and then isolate transformed strains on media that lack uracil, ... MET genes to grow.) Students then use replica plating to determine if expression of MET/Met genes from...

  14. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...pluripotency marker expression and response to LPS stimulation, respectively. Primary cell type Gene targeted... a fluorophore or tag. To utilize HDR, a donor template containing the desired sequence flank by homology...Nanoblades which carries a gRNA, Cas9, and a HDR donor template DNA can be generated by incubating Nanoblade particles...facilitated HDR with either a ssDNA or dsDNA donor template. In vivo editing of mouse zygotes and mice with...

  15. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...pockets. They allow researchers to identify and isolate very specific biological targets. However, as popular...identical antibodies (See Fig 1.). rAbs are a relatively new technology that takes advantage of DNA sequencing... NOT align with the atlases/literature and is unrelated to the target protein, we categorize the antibody...strong signal. However, exposure time and its relationship to signal depend on what you’re comparing your...

  16. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...donor-acceptor pair and a change in FRET efficiency correlates with a change in the distance and/or orientation...available. Fortunately, several excellent reviews have collated data from various papers to make the decision ...conformation of the probe. More on these biosensors later on). Experiments using intermolecular FRET probes...simultaneously would further increase the amount of correlated information available from a cellular process...

  17. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...embark on or continue their careers in science-related fields. In particular, two recent events have the...note is a ruling in August by the National Labor Relations Board to allow graduate students who work as teaching...university and alterations to the student-university relationship, along with, of course, union dues. Time will...+ adjustments for regional cost of living and inflation) and benefits that were laid out by a 2014 National...

  18. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...typically used (e.g., use 5 mL medium on a 10 cm plate). By reducing the culture volume into which the ...transfection and should give rise to a surviving population of approximately 20–50% of the packaging cells...vector using the harvested viral solution as a PCR template. Regardless of the type of virus you’re working..., and allow you to standardize your experiments later. Stay tuned to the Addgene blog and be sure to check...

  19. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...barely fits into AAV when you add in a gRNA and regulatory elements. To solve this problem, the Zhang lab...throughout the 23-base sequence. Double mismatches ablated Cpf1 activity, except when they were present in...future edits. In contrast, since Cpf1 cleaves relatively far away from the PAM, NHEJ might retain the ...mechanism. To prevent new editing post-HDR, repair templates should remove the PAM sequence. Many research ...

  20. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...genetic tool for researchers to conditionally manipulate gene expression in vivo using site-specific recombination...orientation, the SSR will excise the DNA fragment. By manipulating the number, orientation, and type of target ...morphology, and circuit mapping of a targeted neuronal population.    Figure 2 (modified from Schnutgen...study the physiology or behavior of certain cell populations.  Acknowledgement:Through a partnership with...
Showing: 401 - 420 of 787 results