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Showing: 381 - 400 of 678 results

  1. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...introduction to pCri, please see the original publication cited below. pCri plasmids are available either...comprehensive review of affinity tags and microbial applications." Biotechnology journal 7.5 (2012): 620-634....

  2. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ... new method to explore the inner structures of delicate plants and other organisms without destroying ...competition. This competition has gained so much public interest for its stunning images that is has been...

  3. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ...complete with all the fixings), as well as plenty of delicious desserts. We enjoyed a video highlighting the ...course of scientific research. What was once a complicated process of requesting and sharing plasmids has...

  5. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...neurons expressing nLightG or nLightR before/after application of NE (10 μM) and corresponding pixel-wise ΔF.... Sensitivity In ex vivo studies on mouse brain slices, nLightG and GRABNE were more sensitive than nLightR...

  6. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    Published
    June 8, 2023, 1:15 p.m.
    ...and sorting. Reference images can be viewed by clicking on the links in the "Agree" and "Disagree" columns... repository for reagents, protocols, panels, publications, software, and datasets. It’s also a great place...

  8. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    Published
    March 27, 2014, 5:14 p.m.
    ...of hues. While those tools are great for many applications, Verkhusha and his lab at Albert Einstein College...Verkhusha’s team reported another advance in the application of near-infrared fluorescent proteins, iRFP670...

  9. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...parasitization of cereal crops. As a result, agricultural applications of these novel biosensors could have a major...CRISPR library from Alec Kimmelman targets mouse metabolic genes with ~6 gRNAs per gene.  CRISPR-Act3.0, ...

  10. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...meetings as opposed to phone calls most closely replicates how you’d interact with team members in person...access data and drafts of manuscripts and grant applications in real-time so they can provide input at the...

  12. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    Published
    March 15, 2018, 12:59 p.m.
    ... a Cas9 nickase and two gRNAs.  For nickase applications, a common question is: how should the gRNAs ...information and a link to the archived webinar, click here. We would like to thank IDT scientists Mollie...

  14. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...is resistant to specific compounds, links to publication references, and cross-references with cell line...manually going through the pdfs of the 26,000 publications cited. If the information seems incomplete or...

  15. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...Interspaced Short Palindromic Repeats. That contrast of simplicity and complexity is reflected in the biology, too...simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer to the programmable...

  17. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...takes minutes. As a result, it is not possible to elicit an immediate response or track the exact time of...beneficial for use in therapeutic or behavioral applications where drawn out effects are required. Targeted...

  18. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ... mutations in single stranded DNA undergoing replication.   DNase on the other hand can digest and degrade...directly use your DNA preparation in any downstream application involving enzymatic reactions, a valid alternative...

  19. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...include training or teaching others, research, and policy development. Every worker at every level does better...all times when making decisions, assignments and polices. Sometimes the boss has to be the boss and make...

  20. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...with the newest CRISPR technologies and their applications can be exhausting. A quick search for "CRISPR... genotyping assay (such as Sanger sequencing). Click here for a useful reference.  More FAQs, CRISPR ...
Showing: 381 - 400 of 678 results