... a single gene to cells. Still in bacteria, which do not produce BV, an additional gene coding for heme...proximity of the N- and C-termini. Latest miRFPnanos9 do not yield to (m)iRFPs in cellular and molecular brightness...

...selection: labeling markers that your cells of interest do not have, and selecting for the cells that are not...cytosolic proteins alongside the intranuclear ones. You do not need to use a separate fix/perm cytosolic procedure...

...RGD-binding integrins at non-ligand-binding sites and do not interfere with integrin function. Overall, the...function of mouse αVβ3, again the first known reagent to do so. The 15 hybridoma-derived antibodies fall into...

...repair, these methods do not involve double strand break (DSB) formation and do not require donor DNA,...

...GFP, or RFP. Cells that express the construct but do not undergo recombination can be detected by immunostaining...transgene expressing either YFP or CFP, and cells that do not express Cre fluorescing with the default RFP....

...small probes that are entirely genetically encoded do not exist, but the development of non-natural fluorescent...functional heterotrimeric G-alpha subunit fusions that do not tolerate N- and C-terminal fusions (Adjobo-Hermans...

...USA and Europe, something that I would not able to do without a program like this. However, I realized ... she does research at UK in the same field that I do: prostate cancer. She has guided me in my next steps...

...methods to quantify DNA? Here we cover five methods to do this. 5. Plasmids 101: Plasmid Incompatibility by...

... started!  I learned a lot about creative ways to do science communication and outreach - if you'd like...

...remember to step back and ask, "Why did I start doing this in the first place?" Many thanks to our guest...

...about changes influenced by feedback. If you don't do this, employees will stop providing feedback pretty...

...less than an hour. However large proteins typically do not transfer as well using this method.  Finally,...overexpression systems) while negative controls are those that do not naturally express the protein or have had gene...

...limit, some like prime editing's PE2 enzyme do not. So how do you fit a big gene into a tiny vector like...

...little to no signal in the liver sample, which you do (C), so your antibody is validated! Genetic Strategies.... The simplest version would be to use cells that do not express your protein of interest endogenously...

...and then derive mice from these engineered ESCs. To do so, the engineered ESCs would be injected into developing...mechanisms rush in to repair the damage. If all they can do is stitch the two broken ends together through non-homologous...

... infect and then kill bacteria they enter. But to do so, the phage must recognize a specific part on the...

...later. Protein purification specialists recommend doing some research, notably determining the subcellular...

...business partners, without whom Addgene could not do what it does.  Melina talked about her grandfather...

...Your next cool experiment requires some AAV.  Where do you start? Plasmids of course! You just need three...

...species. Some Cas proteins, like LbCas12a or AsCas12a, do not require a tracrRNA. sgRNA: “Single-guide RNAs...