...transcription of the phage repressor cI. Repressor cI inhibits transcription from phage promoter pR, preventing...

...specificity landscape of the SH3 proteins using peptide-phage display and deep sequencing, revealing that they...small molecule binding screens, peptide arrays, and phage display selections. In addition to these constructs...

...editors By Susanna Stroik The Liu lab recently used phage-assisted continuous evolution and protein engineering... here!  Doman, J.L., Pandey, S., et al. (2023). Phage-assisted evolution and protein engineering yield...

...CRISPRi screens to identify essential genes and phage host factors, and identify sequence-specific toxicity...

...able to destroy DNA viruses, similar to the anti-phage CRISPR effect observed in bacteria. Learn more about...

...endo-lysosomal trafficking of adeno-associated virus/phage." Journal of Biological Chemistry (2012): jbc-M112...

... to maximize the mutation rate. For example the Phage Assisted Continuous Evolution (PACE) method requires...of the thioredoxin-binding domain (TBD) from bacteriophage T7 DNA polymerase increased the processivity...

...single array to target the genome of the invading phage pathogen. Over the past decade, CRISPR tools have...

... sequence if not methylated (if it comes from a phage for instance). Aside from restriction modification...

...and specificity. In this first, the Liu lab used phage-assisted evolution selection systems to generate... LW, Zeng J, Bauer DE, Doudna JA, Liu DR (2020) Phage-assisted evolution of an adenine base editor with... indel formation 1.5-2 fold, the team fused bacteriophage protein Gam to the N-terminus of BE4 (Komor ...Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors...

...challenge, the Uppalapati and Woolley lab developed a phage display technique using small scaffold proteins ...

...occur naturally in any known genome other than P1 phage, and is long enough that there is virtually no chance...from the P1 bacteriophage: the Cre recombinase and a loxP recognition site. The P1 bacteriophage uses these...Let us know in the comments! References 1. Bacteriophage P1 site-specific recombination. I. Recombination...in mammalian cells by the Cre recombinase of bacteriophage P1. Sauer, B. and Henderson, N. 1988. PubMed...

...2018). xCas9 was generated by the Liu lab through phage-assisted evolution, specifically with the goal of...

...method, developed by Invitrogen and based on lambda phage integration sequences, relies on site-specific recombination...

...antibodies. The selection process is often performed via phage display, yeast display, ribosome display, or mammalian...

...hypercompact genome editor from the Biggiephage clade of huge phages. In this article, they investigated...as in human and plant cells. Casɸ from Biggiephage can eliminate competing mobile genetic elements...

... hospitals. Dr. Qimron proposes using bacterial phage vectors with CRISPR/cas9 sensitizing gene cassettes...

...by using 6-12 phage PP7 coat protein binding sites and 24 copies of the MS2 bacteriophage coat protein ...Synthetic Biology Center have deposited a set of 11 phage integrases which act irreversibly on their cognate.... These pairs are closely related to the lambda phage integrase system which is the basis for Gateway ...authors recently used computational protein design, phage display and high throughput binding assays, to engineer...

...Central PMCID: PMC3760253. 2. Rohwer, Forest. "Global phage diversity." Cell 113.2 (2003): 141. PubMed PMID:...more abundant are the Earth’s estimated 1031 bacteriophages (viruses that infect bacteria), many of which...

...Antibiotic Resistance Genes Are Used in the Lab Probe Phage Genomes for Host Binding Proteins Learn How to Use...genes from one bacterium to another through bacteriophage (viruses that only infect bacterial cells), ...