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  1. Fluorescence Titering Assay

    Type
    Protocol
    ...cells were transduced with a range of dilutions of pHAGE-TO-dCas9-3XmCherry . 72 h post transduction, cells...

  2. Lentivirus Production

    Type
    Protocol
    ...μL total per 10 cm dish). Using transfer plasmid pHAGE TRE dCas9-KRAB (total μg of plasmid DNA 27.8 μg)...

  3. Colony Formation Titering Assay

    Type
    Protocol
    ...indicated serial dilutions of the lentiviral vector pHAGE EF1α dCas9-KRAB . The following day, cells were ...
Showing: 1 - 3 of 3 results