...available! Interested in purifying Cas9? Check out pET-28b-Cas9-His from Alex Schier's lab, designed for...generated mix of fluorescent proteins, determined by competing recombination events at the genetic level. Upon...

... These are sandwich, competitive, direct, and indirect. Sandwich and competitive ELISA use antibodies ...et al., 2019.   Competitive ELISA Similar to a sandwich ELISA, the competitive ELISA is also used ... be adapted to a competitive format. Figure 2: Overview of using Competitive ELISA to quantitatively...epitopes on the target protein, so they do not compete with each other. This antibody is also conjugated...to your protein of interest) will bind to the competing antigen in the wells while the protein-antigen...protein of interest in the original sample. The competitive ELISA is best used for detecting antigens that...sandwich ELISA. While the most common type of competitive ELISA is a modification of the sandwich ELISA...

...antibody can be used. In a competitive ELISA a multiwell plate is coated with a competing antigen (green).  The...antibodies are used.  Competitive ELISAs Scientists typically use a competitive or inhibition ELISA when... of ELISA are direct, indirect, sandwich and competitive. In a direct ELISA a reporter enzyme conjugated...sample, the less antibody available to bind to the competing antigen. In direct, indirect and sandwich ELISA... level of antigen in the sample whereas in a competitive ELISA the reporter signal is inversely proportional...sandwich ELISA. In this method, the target antigen competes with a reference antigen for binding to a primary... direct, indirect, and sandwich ELISAs, in a competitive ELISA the signal intensity is inversely proportional...

...scientific art competitions where creations can go on display for all to enjoy. The largest competition of this... University’s Annual Art in Science competition. This competition has gained so much public interest for...moonlighting as artists can compete in the Nikon Small World Photomicrography Competition - a 40 year old international...been featured in Time magazine . Other smaller competitions are now taking place and gathering momentum,...international competition that last year received over 2,000 entries. Do you mix your passion for art and...

...compartments pCEP4CyPet-MAMM Cyan Mammalian Expresses mammalian optimized CyPet pCyPet-His Cyan Bacterial...monomeric Venus pCEP4YPet-MAMM Yellow Mammalian Expresses mammalian optimized YPet pYPet-His Yellow Bacterial...Bacterial Expresses CyPet with C-terminal His tag SCFP3A Cyan Mammalian Express a gene of interest fused...Bacterial Expresses YPet with C-terminal His tag SYFP2 Yellow Mammalian Express a gene of interest fused...

...genetic tools. Mobile-CRISPRi from the labs of Jason Peters, Oren Rosenberg, and Carol Gross provides a streamlined...Mobile-CRISPRi's modular components. Image from Peters et al., 2019 with permission.   Two methods... with an integrated copy of CRISPRi. Image from Peters et al., 2019 with permission.    The team showed...strains and environmental isolates. For example, Peters and colleagues used Mobile-CRISPRi to knockdown...possible with Mobile-CRISPRi. As a proof of principle, Peters and colleagues constructed a 40-member library ...microbiology 4.2 (2019): 212. PubMed PMID: 30675034. Peters, Jason M., et al. "Enabling genetic analysis of...PMID: 30617347. PubMed Central PMCID: PMC6424567. Peters, Joseph E. "Tn7." Mobile DNA III. American Society...

...survival in the crowded and competitive natural environments outside the petri dish. Although we usually...beneficial traits that allow them to survive in competitive environments. Plasmids derived directly from ...that allow their host organisms to thrive in competitive environments. The genes encoding these functions...triumph over your neighbours is to eliminate the competition via the production of toxins and antibiotics....There are less aggressive ways to survive in competitive environments, however, such as taking up an unoccupied...

...all bacteria are naturally competent to take up DNA, they can be made competent through chemical manipulation...Addgene’s blog to learn about making your own competent cells and our protocols page to learn about bacterial... The phagemids are packaged into replication-incompetent phage particles with assistance from a ‘helper... A., and Karen Guillemin. "Multiple phases of competence occur during the Helicobacter pylori growth cycle...Meibom, Karin L., et al. "Chitin induces natural competence in Vibrio cholerae." Science 310.5755 (2005):...

... and tested in the “market” by competing in business plan competitions. After much learning, the focus...plan competition and advance investors to the next stage of diligence." In business competitions everything...concept development and participating in business competitions. When the team was unable to secure enough money...CV tips - LabSpaces Blog Find a Buisness Plan Competition Good Relations - PR for the Biotech Business ...

...200 ul pipettor is set to 200 ul, wiped down with 70% ethanol, and a box of sterile truncated pipet tips...the pipettor, placed into wells, and partially dislodged from the agarose plug by rapid pipetting, as ...rapidly punch out cell clones with sterile truncated pipet tips. Indeed, an experienced operator can pick 48.... We highly recommend the use of an electronic pipettor with variable spacing (such as the Viaflo from...

...Prior to March 2020, internships were a fiercely competitive endeavor. Due to the COVID-19 pandemic, students...college, and graduate students. In order to gain a competitive advantage this cycle, consider the advice below...counterintuitive since that approach seems to create competition for your own spot. True! But you create a network...sense approaches that will prepare you for the competitive landscape beyond college. They will also serve...graduation. While these suggestions increase your competitive edge, they alone, are no guarantee of success...

...plasmids, they are generally electroporated into electrocompetent cells to ensure the highest possible transformation...use for your transformation(s). The amount of competent cells you need total and per transformation may...liquid culture, all of the bacteria can grow and compete in the same container, making it easier for a smaller...be its own colony, and will not be in direct competition with other colonies containing smaller, recombined...recommend ensuring the cells used are meant for repetitive sequences (such as STBL4 or Endura Duo), growing...

...curve, avoid pipetting small volumes (< 2 µL) or volumes too large for a standard micropipette (> 1,000 µL...2. When preparing a dilution series, use a new pipette tip for each step and mix the samples well by vortexing...readout. When preparing a standard curve, change pipette tips and mix thoroughly by inversion or vortexing...series, use consistent dilution factors, change pipette tips regularly, and mix samples very well.  Use...

...the same replicon are incompatible because they compete for the same replication control machinery within... concept is a little more complex than simply competing for replication control factors and tends to be...plasmids with the same centromere-like binding region compete for the same CBPs and NTPase to correctly partition...blocking replication of another and one plasmid outcompeting the other plasmid for replication or partitioning...machinery (Novick, 1987). In cases where one plasmid outcompetes the other, usually a smaller, higher copy plasmid...

...want to use electro-competent cells instead of the more common chemically-competent cells. You should perform...Follow the manufacturer’s instructions for your competent cells. For most standard cloning, you can transform...transform 1-2μl of your ligation reaction into competent cells such as DH5alpha or TOP10. If using much ...getting colonies, you might want to use higher competency cells. Additionally, if your final product is...

... appreciated the creative uses of old posters, pipette tip boxes, and more (stay tuned for a few blog ...sustainability in the lab!). The @ladiesinlabcoats used a pipette tip box and microcentrifuge tubes to make Eppi ...labchristmas #cute #reindeer #DeckTheLab #eppendorf #themuppets #scifun #funny #ChristmasScene #womenwhoscience...all who participated in the 2018 #DeckTheLab competition. The four winners will receive a holiday care...

...forming a vector that could create replication competent virus. Even though this system was efficient at...potential risk for the generation of replication competent lentiviruses (RCL) especially if you are working...positive cells could make the virus replication competent. The 3rd generation of lentiviral vectors was...8 kb) Potential for generation of replication-competent lentivirus (RCL) Efficient gene transfer Potential...Potential for insertional mutagenesis: Replication-incompetent lentiviruses with human tropism can still infect...

...lab. Iron Chef-style competitions Every few months, we’ll have a cooking competition featuring a selected..., which is either an indication of the stiff competition or says something about my cooking.   This is... to organize. We’ll dedicate a post to these competitions in the future, and hopefully include some of...

...great approach to fully understand and acquire competitive skill sets in a myriad of professions is to engage...Mentorship Leadership Science writing competitions (e.g. Access to Understanding)   Writing...Communication Networking Publicity Business competitions   Leadership Management Marketing ...opportunity to tailor their career paths for competitive positions both within and outside academia. Use...

... into your data. Try different types of pipettes and pipetting methods to find what’s most consistent ...plate in a way that will tell you if you have pipetting errors on the edges of the plate. Compare the ...multiple configurations can help you identify pipetting or evaporation errors. And if time allows, you...BioRender.   Passive Reference Dyes Whatever your pipetting method, some variation will occur. A passive reference...