... was in its infancy, but the eye was identified early on as an ideal target. It is an easily accessible...dog, was characterized and found to suffer from early and severe visual impairment that faithfully recapitulated...for ocular conditions. However, AdV was abandoned early on due to its considerable immunogenicity and its...candidate therapies for these diseases. Riding on the early successes of retinal gene therapy, an ever-growing... to name a few. CRISPR-based tools are also particularly well suited to be combined with AAV for in vivo...clinical study combining these tools may start as early as next year. Twenty years after falling from grace...

...zebrafish zygote transforms into an organism with a clearly established body plan including head, eyes, and...lineage tracing defined a high-resolution map of early embryogenesis in time and space, and was also used...mapped using single cell RNA sequencing approaches (Harland, 2018). Several different methods have been developed...popular outside of developmental biology, and are particularly well-suited for neuroscience investigations....Academic Press, 2016. 219-244. PubMed PMID: 27443928. Harland, Richard M. "A new view of embryo development and...Philipp J., et al. "Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy...

...the best things an early career scientist can do is to get started using LinkedIn early and brush up on ...your science. While Reddit isn’t for everyone, particularly if you’re more interested in selling a product...started watching the questions and comments go up early in the day, and the moderators were on top of any...started watching the questions and preparing answers early in the day. Although I didn’t start answering until...best practices for LinkedIn networking. I was an early adopter of the LinkedIn blog format and it is a ...

...surrounding genes. However, there are ways to create scarless mutations, ones that don’t leave any undesired...CRISPR-Cas9 technology has also aided in the creation of scarless mutations, both in stand-alone systems (Jiang ...colonies turn subtly blue or magenta. This is particularly valuable when first optimizing conjugation in... Allelic exchange is a great technique for the scarless modification of bacterial genomes. It can accomplish... New Suite of Allelic Exchange Vectors for the Scarless Modification of Proteobacterial Genomes." Applied...Chris R., and Kristala LJ Prather. "The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome...

...cutting most anywhere within the RNA will do. Similarly, if you want to knock out a gene, a frameshift...frameshift mutation introduced anywhere within the early coding region will do the job. What matters for these...you choosing between efficiency and location.   Nearly PAM-less editing   The Kleinstiver lab sought to...and several of the other Cas variants described, nearly all genomic landscapes should be editable with ...Paquet, D., Teo, S. et al. Precise and efficient scarless genome editing in stem cells using CORRECT. Nat...

...postdocs this will lead to a raise in salary, particularly given that this has precipitated increases to...leading the process despite the recent advance. Similarly, grad students throughout universities that are...of institutional responses to the FLSA ruling. Similarly, from purely a salary perspective, while this ... advocate for the opportunities they present. Similarly, ongoing issues that affect all scientists; such...Public Affairs at ASBMB Adam Fagen, PhD - Director, Early Career Scientist Segment at AAAS Marnie Gelbart,...

...many of these studies report correlations without clearly testing whether a certain methylation state causes...expression changes (Sapozhnikov and Szyf 2021, Charlton et al. 2020). Most importantly, TET proteins affect...demethylation! With optimization, this method can produce nearly 100% demethylation without the confounds inherent...Read our CRISPR Guide to Epigenetics References Charlton J, Jung EJ, Mattei AL et al. (2020) TETs compete... https://doi.org/10.1038/301089a0. Galonska C, Charlton J, Mattei AL et al (2018). Genome-wide tracking...

... Bdnf is expressed early in response to neuronal activity (hence, “immediate-early”) and consequently ...highlight this advantage, they selected the immediate-early gene Bdnf, which encodes brain-derived neurotrophic...IV overexpression variants were other immediate-early genes associated with neuronal activity, such as...sgRNA library and were subsequently cultured for nearly two weeks to allow differentiation to occur. CD8...

...created a stir earlier this year when it announced its data access and sharing policy. Since early March, the...efficiently trawled their own data? A distinct (perhaps similarly impoverished lab) might apply different or novel...requirements imposed by publishers and funders. Clearly, it is in our interest to ensure that our data ...PLOS’ Bold Data Policy - by David Crotty (The Scholarly Kitchen) Read more Addgene blog posts about scientific...

... base of all these questions as there is not particularly good data on how many post-docs there are in...Boston 2015 symposium, the Career Path Panel and the Early Career Researchers in Publishing panel, focused ...waters of career development. Publishing Panel The Early Career Researchers in Publishing Panel brought in...graduate students, postdocs, or professors isn’t nearly enough to reach diversity goals. Universities need...lack of trust in the mentor and this training is clearly not equitable to that of other trainees despite...

...mEGFP, mNeonGreen, mScarlet(-I), and mCherry. Keep the linker sequence short In the early days of GFP applications...instance, Lifeact-mTurquoise2 (Plasmid #36201) or mScarlet-tubulin (Plasmid #85045). We often use restriction...protein is mislocalized. The APT1-mVenus fusion is clearly a better choice to work with. Inserting a fluorescent...dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins." Proceedings...

...Expanding your network of relationships early and often is the most effective tactic a scientist in training...excellent resume. 2) Follow job boards and check them regularly Use your research skills to determine which job...specifically in the position. We frequently receive clearly generic cover letters that expound on bench skills...experience. Most Addgene PhD level job descriptions clearly describe that the roles do not involve bench research...

...reversed chronological style (the latest to the earliest) or a functional one. Pick one that makes sense...students in job searching. Start your career planning early, start now Don't wait until the day you defend to...academia or not, it is even more important to start early. Think about your plan for the next 5 years. Does... might be a tough question to ask now, but the earlier you ask yourself, the better prepared you are for...

...intensity. Signals any higher than this may not be clearly picked up by the sensors. You can adjust this by...sample (right). Negative and positive samples are clearly distinguishable as two separate populations. Created...you are looking at proteins that are expressed regularly in cells and might be upregulated or downregulated...immunotherapies. She is currently a Research Assistant in Arlene Sharpe’s lab at Harvard Medical School.   More...

...AAVrg pAAV-Ef1a-DIO EYFP Karl Deisseroth 11447 AAV5 pAAV-Ef1a-fDIO mCherry Karl Deisseroth 112677 AAV2...pAAV-hSyn-DIO-EGFP Bryan Roth 114472 AAVrg pAAV-hSyn-mCherry Karl Deisseroth 83895 AAVrg pAAV-hDlx-Flex-GFP-Fishell...noninvasive methods for manipulating cell activity, particularly in the brain, and holds promise in the development...

...) career. Had I known about product management earlier on in my career, I’d have realized it was a great...could code the best operations software possible. Similarly, when the developers needed to know information... are many paths to software product management. Early in your career: Try to get software product management...Technologies for the North America region. She is particularly interested in DNA sequencing tech, and the intersection...

...Since its early years, the light microscope has been a powerful tool in almost all disciplines. In biology... that actively engage with the community are particularly successful. Machine-learning denoising of raw...rely on such tools has allowed the community to regularly replicate designs worldwide. Distributing hardware..." bioRxiv. 2020. Diederich, B., Lachmann, R., Carlstedt, S. et al. A versatile and customizable low-cost...

... confidential process among a few individuals.  Early-career researchers especially enjoy the ability ...in journals after formal peer review, with the earlier attention and feedback giving authors the opportunity... KK, Puebla I (2022) A guide to preprinting for early-career researchers. Biol Open. https://doi.org/10.1242..., Hensel Z (2019) On the value of preprints: An early career researcher perspective. PLoS Biol. https:...

...work with a collaborative group, she also joined early on and contributed her expertise in developing reproducibility...others to practice and teach reproducibility An early milestone for the group was the “reproducibility...results, and tracking material downloads. This was particularly helpful for securing funding to support this...reproducibility workshops and lessons learned: Since early 2018 the team has grown to include experts who could...

...look! This stuff works!” These papers can be a particularly good resource if you are considering using a...or Bing it, but, let’s face it, there’s a reason nearly 70% of all internet searches are done via Google.... Addgene keeps a Document of Gratitude from particularly enthusiastic customers who have enjoyed their...combatitive. Not every question is answerable, particularly when it comes to technical questions. BUT, I...