...so come prepared with questions to ask. With a quick Google search for “questions to ask an interviewer... asks you a smart question. There’s no reason you can’t then ask that same question in your next interview...ll probably still be required to give a job talk for a remote interview. Fielding questions can be hard...t be able to see who’s asking a question. Maybe you ask for questions after completing each section of... a popular way to answer behavioral interview questions. STAR stands for situation, task, action, and ...preferred to prepare stories to answer interview questions. Story telling shares many of the same elements...of the theme of my interview stories as the characteristic which the story demonstrates I possess.   Bullet...

...students will have questions about the program and having the time to ask personalized questions might be difficult...pressure off. This question and answer process can also help you to see if the institution is right for you...about to graduate shares the same nightmare: the question “So, what comes next?” For those finishing up ...the right fit for you without the financial and logistical stress that accompanies traveling for campus ... know you have an answer to that previous nosy question! The Application Process Broadly speaking, the...COVID-19 impact all of this?” are all very valid questions, and often make an already complicated process...by meeting with professors - a/k/a Principle Investigators (PIs) - whose labs does research that aligns...

...environment. The “how many?” question lies at the base of all these questions as there is not particularly...addition to highlighting some of the important questions that still need to be answered about the makeup and...first time live tweeting: a fun and surprisingly exhausting experience. As Addgene believes many of the topics... Boston 2015 organizers indicate that the main question they wanted to focus on during the symposium was...post-docs?” You can find some of the more detailed questions the organizers wanted to touch on in organizer...the postdoc work force, problems start with the question of “What is a Post-doc?” The National Academies...his or her chosen career path,” but individual institutions can have many different names for the positions...

...  National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK)  National Institute of Genetics...depositors’ intellectual property, and to help requesting institutions safeguard themselves from undue liability...Hospital  Brandeis University  Broad Institute  California Institute of Technology Cold Spring Harbor Laboratory...Rockefeller University  Salk Institute for Biological Studies The Scripps Research Institute St. Jude Children's...University and Medical Center  Weizmann Institute of Science Whitehead Institute for Biomedical Research Glossary...materials? If so, you should always talk to your institution’s TTO or legal office first. Generally, Addgene...points for broaching this subject with your institution’s tech transfer professionals: Describe to your...

...person. I like to ask questions and think about the best way to answer those questions. I’ve met graduate...about the science allows you to ask more interesting questions and your excitement will in turn inspire...lab? Ask your questions—especially if it’s scary. I’ve been afraid to ask “dumb questions” and to make ...dumb and very basic question was actually a great one. Further, even if a question is a very basic one...postdoc” is, and that “PI” stood for principal investigator. In basic science (as opposed to translational... sciences), people conduct research to answer questions about how the world works. As scientists, we probe...in a lab. Position Common tasks Principle investigator (PI) Runs the lab Writes a lot of grants Varying...

...idea of how to leverage it to ask new and interesting questions in your own lab. 2. Seek multiple mentors... Research Campus of the Howard Hughes Medical Institute. Today's postdoctoral fellows (PFs) face a number...establish your own lab and get an independent investigator grant (e.g. an R01 from the NIH, in the United...fellowship lasts five years. Most universities and institutes annually advertise for Group Leader positions...sufficient data to write a manuscript. Consider questions like: What figures are missing? For example, are...can be found during conversations with senior investigators. Discussions can reveal mutual scientific interests...experiment in your new lab, stop and plan it out. Some questions to answer include: What is the goal of the experiment...

...negotiate for a win-win decision An institute/department is investing in you, and is counting on your research... the institute time and energy that would typically be wasted going through some of the questions we mentioned...a junior investigator turned down a faculty position because none of the offering institutes were able...poor outcomes; not enough funds to get started, wasting funds on unnecessary materials, or even a loss ...specific aims. This is actually why a department/institute is interested in you. Your science will define...based on these findings. Most often we suggest estimating the time it will take to secure your first (R-level...greater than $5,000 but this can depend on the institution/funding source. Major equipment includes large...

...we account for an estimated 2% of global plastic generation. Less than 9% of plastics are ever recycled...mail. Recycle plastic packaging Packaging is a huge contributor to the increase in plastic manufacturing...services don’t offer plastic recycling, specialized companies can recycle plastic-- you just have to find.... This service does cost money, but your institute may still be interested as it offsets the cost of regular...recycling! With the popularity of single-use items, plastic is a substantial portion of lab waste. Despite ...label. To return the box, simply peel your address sticker off the box, remove the ice pack (it’s not recyclable...manufacturing, accounting for 40% of total plastic usage (Geyer et al., 2017). While most single-stream or city ...

...thus individual laser stimulation is essential. In terms of the laser stimulation itself, the output of...battle when using optogentics to answer a research question. The flip side of the optogenetics coin is materials...the dorsal/ventral location of your region of stimulation (the z-axis bregma coordinate). Because the light...it enters tissue, placement above the site of stimulation guarantees that the majority of the opsins will...should be about 0.4-0.5mm above the site of your stimulation. The scatter effect is also an important consideration... polished to significant smoothness to prevent drastic loss of efficiency between patch cable and implant...during the entire protocol, otherwise optical stimulation will be erratic, opsin activation/inactivation...

... host lab or institute provides something that you can’t obtain at your current institute. A common reason...don’t have, or maybe both, a visiting scientist stint may be ideal for you. Here, we will chat about who...scientist, in academia, is someone who visits an institution to perform research there temporarily. The length...The researcher remains employed by their home institute, meaning the host university does not hire or ... pay them (typically). A visiting scientist is distinct from equipment sharing or a collaboration – it...requires re-location of the scientist to the new institute where their typical workdays will be carried out...back to your home lab. Alternatively, the host institute may have a piece of equipment that is custom-built...

...specific statistical test.  Preregistration makes clear the distinction between hypothesis testing work, ... and other questionable research practices (QRPs) can quickly lead us to find statistically significant...encouraged to present that work using hypothesis testing statistical tools that generate a p-value.  As an example...authors are able to make that clear distinction between hypothesis testing and generation, can be sure that...biomedical and psychological sciences were called into question after credible attempts to replicate major findings...instead of the methods. Unreported flexibility and questionable research practices In an attempt to present ...and hypothesis generation, in which data are investigated to look for unexpected trends or differences...

...process. This early research is still relevant to his work at the Broad Institute today, where he helps researchers... to answer their own research questions. Starting at the Broad Institute For his post-doc, Doench worked...towards the Broad. In order to ask interesting biological questions, you need a tremendous amount of technology...with scientists asking biological questions. Doench loves that he still gets to see data as it’s coming ...the Broad Institute here Read other blog posts about CRISPR Learn more about the Broad Institute and the...the Genetic Perturbation Platform at the Broad Institute and has worked with many Addgenies to help improve... School and then applied to work at the Broad Institute: “The model that the Broad was building that had...

...(BRET) were developed by the Johnson Lab . Characteristics of the pHlash reporter protein include: H+...large changes in binding affinity with light stimulation. Indeed oLID showed only a two-fold change in...50 fold change in binding affinity with light stimulation2. The Kuhlman lab created two improved Light ...specific genes via user-defined guide RNAs. Upon stimulation with blue light, the complex recruits a CIB1 ...pUCXKT, removing the stop codons during backbone digestion and providing the missing codons for functional...C-terminal tags. Additionally, by using an adapted destination vector with a heterologous promoter outside of...create an Entry vector containing a promoter for constitutive (CMV, SV40, SFFV) or inducible (CMV-TO) cDNA...

...components into a destination vector in a predefined order. Finally, the destination vectors are used ...the positional groups. Eight final destination vectors, consisting of all four final RVD modules in each... this by selecting the important elements from existing transposon and vector systems and creating an ...transport. pMitoTimer from Yan’s lab, which is constitutively expressed, reports on the balance of biogenesis...fluorescence microscope 8 hours post-pulse. The distinct differences of red-to-green fluorescence in individual...modular system for easier exchange and re-use of existing elements. DNA sequences for a desired component...be used for each Multisite Gateway compatible destination vector for a maximum of 24 elements assembled...

...interest, and stimulate or manipulate responses through neurological means like electric stimulation, drug administration...Due to the soft nature of the intestines and the requirement for digestive motility to not be disturbed...response to both of these stimulations. The best part? The chemical stimulation was sucrose…..sounds like...validated simultaneously in the brain and the intestines of mice. The result? A very cool tool to advance...Anikeeva lab are microelectronic fibers made from thermoplastic polymers that can work with all the bells and...engineering road map of sorts, giving an idea of the stiffness the fibers would need to be, as well as other ...function (the gut twists and contorts during the digestion of food, while brain tissue is relatively static...

...Hubrecht Institute in The Netherlands used the CRISPR/Cas9 genome editing system to correct the cystic fibrosis...homologous recombination in cultured intestinal stem cells of patients with cystic fibrosis (8).   The difference...therapeutic.” There are many fundamental questions about CRISPR/Cas biology still left to answer as well, she says...Repair of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem ...and precise, researchers are now using it to investigate potential therapeutics for complex genetic diseases...adult stem cells isolated from two patients with cystic fibrosis. They were able to demonstrate functional...stable,” said Gerald Schwank, first author of the cystic fibrosis report. “This is a big advantage over ...

...sized digestion products (that would co-migrate on a gel) instead of two different sized digestion products...need/want to put the digestion site in the middle of the PCR product, you would still be able to identify...undigested control can help distinguish between a smear and a faint digestion product. Also, depending on...transduced (MOI = 37) and selected with 1 µg/mL blasticidin for 9 days. Single cells from a stable cell pool...then expanded for an additional 3 weeks under blasticidin selection. Anti-Cas9 Western blotting was performed...cells in the population, so some of the cells will still appear green despite having active Cas9 (see tip... target different regions of your gene. I used existing BRAF gRNA viruses, but you can find a list of ...

...developed this tool, there were so many interesting questions that we could apply [it to].” Using ClonTracer...Library, deposited by Carrie Bhang, a research investigator in the In Vivo Pharmacology group at Novartis...environment” with a strong focus on publishing, but still made it so she could “get exposure to the drug discovery...started using the tool to look at a variety of questions surrounding cancer development, metastasis, and...drug treatments arise during treatment or are preexisting in the parent population prior to the start of...from the same parent cells that represent the preexisting resistant subpopulation 2. Resistant cells among...would have different labels - i.e. they would stochastically arise from different cells throughout the treatment...

...viral expression but may still be sufficient for successful stimulation. Testing opsin expression  Regardless... of Antonello Bonci, Director of the National Institutes of Drug Abuse Intramural Research Program (NIH-NIDA...you want to use optogenetics The most important question to consider is what exactly do you want to use...optogenetics is best used to address a specific question. Optogenetics is a tool and, just like any other...circuit of interest in promoting reward is a self-stimulation experiment wherein mouse initiated optogenetic...in a reward sensation and subsequent repeated stimulation. A spatial memory task, on the other hand, would...opsin of choice in the brain region you wish to stimulate 2) Fabrication and implantation of the optical...

...ligated into Cas9 or dCas9-containing destination vectors. Destination vector options include humanized wt...Addgene. 8. Xie, Kabin, Minkenberg, Bastian, & Yinong Yang. “Boosting CRISPR/Cas9 multiplex editing capability...editing. Multiplexing gRNAs: the basics One common question Addgene Senior Scientists receive is: can I express...ordered assembly of multiple components into a destination vector. The first step in CRISPR/Cas9 Golden ...oligonucleotides specifying each gRNA target sequence into distinct expression vectors using the enzyme BbsI. These...together to allow for ordered assembly into a destination vector that expresses Cas9. This is illustrated...drive ordered assembly into a Cas9-containing destination vector. Note: depending on which method you use...