...insecticidal proteins to the development of renewable biotechnology products and biosensors for herbicides as well...a bacterial expression system for renewable biotechnology products Dr. Idah Sithole-Niang, University ... Lecturer and a Plant Molecular Biology and Biotechnology expert. In order to preserve Cassava yields ...Robertson aims to make use of the CRISPR/Cas9 technology to engineer plants that are able to destroy DNA...Associate Professor (Right), focuses on molecular biotechnology for improving expression of nutritionally and...Bao Ton (center) is interested in applying gene technology to bacteria and plants. Her research involves...water contaminants and to subsequently develop technologies to protect fresh water repositories in Mexico...

...activity mapping with STARR-seq   Although many technologies have been developed for the study of enhancer...available from Addgene in both human and fly versions. Arnold, et al. Science. 2013. PubMed PMID: 23328393 Wu... and mutant library generation in several biotechnologically and clinically relevant bacterial species...SnoopCatcher The development of recombinant DNA technology has allowed molecular biologists to fuse fragments..., such as the food and beverage industry, the bioethanol industry, and the pharmaceutical industry, depend...through Addgene. Stovicek, et al. J Ind Microbiol Biotechnol. 2015. PubMed PMID: 26376869 Jensen, et al. FEMS...modification in Caenorhabditis elegans CRISPR/Cas9 technology has revolutionized genome editing in multiple...

... Osaka University have developed Nano-lantern technology. Nano-lanterns contain a Renilla luciferase variant...but it has precluded widespread adoption of the technology. In 2012, Saito et al. described the first Nano-lantern...luciferase (RLuc8) with Venus. This luminescent technology is based on the principle of bioluminescence ...constructs use the brightest characterized luciferase, NanoLuc, and its substrate furimazine. In addition to the...optogenetic system. Biosensors based on Nano-lantern technology are also very robust, with an eNL-based calcium...ratio. Despite the many benefits of Nano-lantern technology, there are a few drawbacks associated with luminescent... not be appropriate for use with Nano-lantern technology. Multicolor imaging using Nano-lanterns requires...

...Harshana S De Silva Feelixge. Gene therapy technologies hold great promise for improving or potentially...Rapid advances in next generation sequencing technologies have allowed scientists to quickly identify ...Almost all patients have achieved persistent immunological reconstitution with a normally functioning T...been challenging. A variety of vector delivery technologies have been investigated including lentiviruses... delivery tools such as nanoparticles. These technologies can have serious adverse effects, and their ...discovered frequently and recent advances in AAV technology have enabled us to generate new and refined forms...consideration, recent advancements in various AAV technologies have vastly improved AAVs’ potential to become...

... post was contributed by Kevin Esvelt, a Wyss Technology Development Fellow at the Wyss Institute and ...widespread trust in scientists to develop new technologies with care and humility. Nothing would damage...could not be trusted to handle such a powerful technology responsibly. An accidental release would be particularly...Finally, gene drives are an inherently collective technology: because they necessarily alter the shared environment...Thank you to our guest blogger! An inventor of technologies that harness evolution, Kevin Esvelt studies...ensuring that gene drives and other collective technologies are developed safely and transparently.  For...101: a blog series on the basics of CRISPR-Cas technology CRISPR Resources from Addgene   ...

...implications of using these technologies, but also the rapidity of the technologies’ adoption by the scientific...amongst the Nobel prize winners for developing the technology. Though these case studies are “old,” they lead... learning addresses the dilemma of creating a technology that could eventually replace scientists themselves...Addgene’s role in the rapid dissemination of these technologies, proposing that “Addgene, with its easy, fast...CRISPR-Cas plasmids, has been a key driver of the technology.” As an Addgene vet of 12 years, I’m very familiar...decline in the competitiveness of U.S. science and technology.” This last section of the book, Future Science...

...in the malaria community for the better (Nat. Biotechnol., 2014). Given the timing, it shouldn’t be a ...yet. As a genome editing tool, the CRISPR/Cas9 technology has been surrounded by a whirlwind of research... sgRNAcas9. Top tools for transferring CRISPR technology to a new organism: For obvious reasons you can...developed with the purpose of applying CRISPR technologies to new organisms and will help you whether you...Report your findings to help everyone else. CRISPR technology has reached into so many different communities...falciparum using the CRISPR-Cas9 system." Nature biotechnology(2014). PubMed PMID: 24880488. 2. Hsu, Patrick...specificity of RNA-guided Cas9 nucleases." Nature biotechnology 31.9 (2013): 827-832. PubMed PMID: 23873081....

...contributed by Steph Guerra, a AAAS Science and Technology Policy Fellow at the Veterans Health Administration...roles and my current work as a AAAS Science and Technology Policy Fellow (STPF) at the Veterans Health Administration...these resources compiled by the AAAS Science and Technology Policy Fellowship Program to learn more about...could understand how to make new treatments and technologies accessible to patients. And what better place...guest blogger Steph Guerra, a AAAS Science and Technology Policy Fellow at the Veterans Health Administration... Guerra, PhD, is currently a AAAS Science and Technology Policy Fellow at the Veterans Health Administration...particularly interested in driving scientific and technological innovation that is accessible and equitable ...

...CRISPR-Cas nucleases in human cells." Nature biotechnology 31.9 (2013): 822-826. PubMed PMID: 23792628....on-and off-target genome editing." Trends in biotechnology 33.2 (2015): 132-140. PubMed PMID: 25595557....RNA-programmed Cas9 nuclease specificity." Nature biotechnology 31.9 (2013): 839-843. PubMed PMID: 23934178....off-target cleavage by CRISPR-Cas nucleases." Nature biotechnology 33.2 (2015): 187-197. PubMed PMID: 25513782....of zinc-finger nuclease specificity." Nature biotechnology 29.9 (2011): 816-823. PubMed PMID: 21822255....integrase-defective lentiviral vectors." Nature biotechnology 33.2 (2015): 175-178. PubMed PMID: 25599175....breaks induced by engineered nucleases." Nature biotechnology 33.2 (2015): 179-186. PubMed PMID: 25503383....

... of Rogue Bioethics. On November 25, the MIT Technology Review dropped a bombshell report. A scientist... is unethical to use scientifically unproven technologies to edit a human embryo, or have a woman carry...controversial and ethically fraught rapidly emerging technology. We could do this for CRISPR/Cas9 – or any other...germline editing, we will initially limit the technology to research centers engaged in long-term, mandatory...review and decide on the future of genome editing technology. We must stop punting decisions on scientific... inform her work on emerging issues in novel technologies, including self-driving vehicles, synthetic ...

...Gram Negative. Technology Networks (2019) https://www.technologynetworks.com/immunology/articles/gram-...

...treatment can lyse cells. Alcohols, like ethanol or methanol, fix and permeabilize in a single step by...sample (1). When combined with cell sorters, this technology can be used to isolate a specific subset of cells...and further analyze that subset of cells. An immunologist, for example, might be interested in the lymphocytes...cell into distinct populations (Figure 2). An immunologist who studies B cells, might then gate the B cell... Cytometry: An Overview. Current Protocols in Immunology 120: . https://doi.org/10.1002/cpim.40 Picot ...retrospective, fundamentals and recent instrumentation. Cytotechnology 64:109–130 . https://doi.org/10.1007/s10616...

...you want to read more content related to CRISPR technology, subscribe to our CRISPR blog content.   In this... dCas12f to VP64. Read the paper in Nature Biotechnology Find the Cas12f-GE plasmids Programmed genome...of target genes and cell types. Their Nature Biotechnology paper showcases an improved design of the guide...editing efficiency. Read the paper in Nature Biotechnology Find the epegRNA plasmids Enhanced prime editing... paired pegRNAs.  Read the paper in Nature Biotechnology Find the PRIME-del plasmids  Deletion and replacement...expression in the liver.  Read the paper in Nature Biotechnology Find the PEDAR plasmids   Additional resources...

...rapidly decode the complexity? The CombiGEM-CRISPR technology was developed with the goal of providing an easy-to-use...This blog post will introduce you to this new technology, and guide you through the basics of CombiGEM-CRISPR...particularly interested in developing and applying new technologies to interrogate and understand complex biological... off-target effects of CRISPR-Cas9." Nature biotechnology (2016). PubMed PMID: 26780180. PubMed Central...lentiviral CRISPR-guide RNA library." Nature biotechnology 32.3 (2014): 267-273. PubMed PMID: 24535568....combinatorial genetics in human cells." Nature biotechnology (2015). PubMed PMID: 26280411. PubMed Central...

...about Mammalian shRNA Tools for RNAi! Cre-Lox technology CAG-GFP-IRES-CRE (Plasmid #48201) This retroviral... and Cre-recombinase. The Cre-lox system is a technology that can be used to induce site-specific recombination...knockout, and selection marker removal. CRISPR technology pdCas9-humanized (Plasmid #44246) Expression ... the pLKO.1 vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid #17408)   This...lentiviral prep from the Addgene viral service. CRISPR technology lentiCRISPR v2 (Plasmid #52961) This is a 3rd...Maetzig T, et al. Gammaretroviral Vectors: Biology, Technology and Application. Viruses. 2011. PubMed PMID: ...

...University in Lisbon, Portugal. She leads the Glycoimmunology group based at the Research Unit on Applied...reported that PMM2-CDG patients suffer from immunological defects. These are characterized by a predisposition...play an essential role in almost every aspect of immunology, participating in events such as cell development... J., dos Reis Ferreira, V. & Videira, P. A. Immunological aspects of congenital disorders of glycosylation...winners! Paula Videira is the team leader of the Glycoimmunology group at UCIBIO, FCT-UNL, Portugal. Her main...role of glycans. Follow her on Facebook: @glycoimmunologygroup.     Michael McMurray is an Associate Professor...

... hood with a disinfectant (most people use 70% ethanol, but you can also use Incidin wipes) before and...everything that’ll be brought under the hood with 70% ethanol, including your glove-covered hands! You should...spills do occur, they should be wiped with 70% ethanol immediately. Finally, avoid sticking pipettes into...Most cell culture media contain the pH indicator phenol red (pH range 6.0 (yellow) - 8.0 (red)). Blood ...is particularly interested in Glycobiology and Immunology. You can find Sana on LinkedIn.   References ...et al. "Gene expression profiling analysis of bisphenol A-induced perturbation in biological processes...

... covers a brief review of different basic BE technologies and their adaptation for plant genome editing...State University Kutubuddin Molla is an Ag-biotechnologist in the National Rice Research Institute, Cuttack...CRISPR-Cas9 cytidine deaminase fusion." Nature biotechnology 35.5 (2017): 441. PubMed PMID: 28346401. 6. ...with a Cas9-cytidine deaminase fusion." Nature biotechnology 35.5 (2017): 438. PubMed PMID: 28244994. 7. ...fusion of nCas9 and human APOBEC3A." Nature biotechnology (2018). PubMed PMID: 30272679. 8. Hua, Kai, ...scope in rice by using Cas9 variants." Plant biotechnology journal (2018). PubMed PMID: 30051586. 9. Hua...

...group developed the original iteration of this technology in 2007. Used to label neurons in the cerebellum...cerebellum of mice, the team appropriately named their technology Brainbow. Brainbow 1.0 used the fluorophores ... promoter. Zebrabow is an extremely versatile technology as recombination can be induced in developing...cells. Tetbow optimizes Brainbow for current technologies Tetbow (Sakaguchi et al., 2018) overcame the...multiple fields of view, whereas previous Brainbow technologies were useful for discriminating neurons within... continue to develop new iterations of these technologies, expect to see systems that use newer, more ...

...CRISPR and epigenetics As with many TALEN-based technologies, the advent of CRISPR has made targeting much...modification is certainly not the only CRISPR-based technology designed to alter gene expression. Fusing dCas9... genes from promoters and enhancers. Nature Biotechnology 33:510–517 . https://doi.org/10.1038/nbt.3199...regulatory elements in the human genome. Nature Biotechnology 35:561–568 . https://doi.org/10.1038/nbt.3853...programmable TALE-TET1 fusion proteins. Nature Biotechnology 31:1137–1142 . https://doi.org/10.1038/nbt.2726...modifications at endogenous enhancers. Nature Biotechnology 31:1133–1136 . https://doi.org/10.1038/nbt.2701...