...What's a lab group in industry? The collaborative nature of working in industry and how delegating can...Crystal’s exciting career path, to gain insight into industry research, and to get advice on how to succeed ...Advice to scientists who are planning on working in industry. The importance of recognizing and leveraging ...adapting to needs as they arise. Tips on applying for industry jobs and deciphering job listings. Mobility between...Podcast.      Additional Resources from Addgene Find more management resources in our Mentoring for Scientists...

...and individual plasmid formats A first for a library at Addgene, you can also order the individual lentiGuide...all-in-one (lentiCRISPRv2, expressing both Cas9 and an individual gRNA from this single vector) and guide-only ...in the first subset of the pooled library. The individual plasmids will be useful when screening for phenotypes...themselves to screening in a pooled setting. The individual plasmids will also be useful for imaging or gene...expression assays necessitating the screening of individual plasmids separately. Additionally, this set can... can be used for cherry-picking individual gRNAs that score as hits in pooled screens, facilitating more...follow-up. We hope the biological community will find these reagents to be useful. Happy screening! Many...

...has been referenced in other publications, you'll find a link to the list of citing articles under the ...article list you can click on the PubMed link to find the article abstract and more. We've also included...into a previously deposited vector - then you'll find the plasmids listed below the citation. An example...Barcoding cells using cell-surface programmable DNA-binding domains" publication.  If you want to see this...identify relevant protocols and experimental data Find other related plasmids in the "Associated Plasmids... Happy researching! If you need any assistance finding plasmids on Addgene's website or would like to ...

...available to industry scientists  While we’ve focused on expanding COVID-19 plasmids to industry scientists...University became openly available. Find plasmids available to industry scientists To make plasmid sharing...available more broadly: to scientists in academia and industry. As a result over 90% of Addgene’s COVID-19 resources... to Addgene’s new approach of working with the industry-based scientific community. Conversations with...to the scientific community - both academic and industry scientists. This partnership complemented each...we have other plasmid collections available to industry? Over the past 8 years, several organizations ...sharing more open for academic and industry scientists, we’ve worked with many scientists, tech transfer offices...

...Addgene.org Read our CRISPR guide Find CRISPR plasmids for your research Find links to CRISPR references ...bison wanders into the campsite, leave your samples behind! Paul and Anastasia (Potanina) Neddersen characterized...thermostable between 31-100°C. They published their finding in a bioRxiv article and in PNAS. Two other thermostable...the van der Oost lab. Phylogenetic comparison indicates that like GeoCas9 and ThermoCas9, IgnaviCas9 is...lavamentivorans, whose optimal growth temperature is 30°C. Find IgnaviCas9 here! What can you do with IgnaviCas9...

...of the juicy and important details that you won’t find in the final publication and we have already collected... AAV Data Hub grows, we hope to mine the data to find patterns that we can share with you about promoters...

...you’ll find Addgene’s newly launched search engine results much better. Here, I wanted to find a specific...plasmids with more than the indicated number of requests Availability to Industry: Restrict search to plasmids...Finding the best plasmid for your research can be a daunting challenge. With over 80,000 deposited plasmids...plasmids from around the world, it can be hard to find your favorite gene, promoter, serotype, or a myriad...articles. From paper to plasmid Authors regularly indicate that their plasmids are available from Addgene... show the range of different terms we can use to find materials from this publication. Searching for the... the title of a paper works You can also find the associated plasmids through the article’s PubMed ID...

...homeobox gene 1 induces expression of insulin genes in liver and ameliorates streptozotocin-induced hyperglycemia...Addgene.org Visit our Stem Cell Pages Find Viral Vectors for Your Research Find Plasmids from the Yamanaka Lab...reprogramming factors- OCT4, SOX2, KLF4, and C-MYC - that induce a differentiated somatic cell to revert back to...Addgene’s stem cell page. On this page, you can also find a table with a list of methods and the species they... hair cells [31] Neurons [32, 33, 34] You can find a variety of plasmids for differentiation here. ... cases where the objective is to fix a disease-inducing genetic mutation in a patient, trying to transdifferentiate...PubMed PMID: 16810240. 2. Takahashi, K., et al., Induction of pluripotent stem cells from adult human fibroblasts...

...depositing plasmids pre-publication Find molecular biology protocols Find eBooks on plasmids, CRISPR, viral... sentences that I found hard to understand or individual words that are not appropriate for the given ... attention to details and use a checklist for individual sections of the paper (see figures). After finishing...speaking to a colleague in the office next door. So be kind, helpful, and thorough in reviewing the paper of...Magdalena Julkowska did her Ph.D. on salt-stress induced responses in Root System Architecture of Arabidopsis...Prof. Mark Tester, where she focuses on (1) salt-induced changes in root-to-shoot ratio in Arabidopsis, ... Boyce Tompson Institute.  References Baldwin, Melinda. "Credibility, peer review, and Nature, 1945–1990...

...disadvantages led to the creation of induced pluripotent stem cells or iPSCs. Induced pluripotent stem cells iPSCs...Addgene.org Visit Our Stem Cell Page Find Plasmids for Reprogramming Find Plasmids for Differentiation ...works led to a Nobel Prize for Dr. Yamanaka in 2012. Find plasmids for generating iPSCs iPSCs and ESCs closely...: 17989069. 8. Takahashi, K. and S. Yamanaka, Induction of pluripotent stem cells from mouse embryonic...PubMed PMID: 16904174. 9. Takahashi, K., et al., Induction of pluripotent stem cells from adult human fibroblasts...72. PubMed PMID: 18035408. 10. Yu, J., et al., Induced pluripotent stem cell lines derived from human ...Medvedev, S.P., A.I. Shevchenko, and S.M. Zakian, Induced Pluripotent Stem Cells: Problems and Advantages...

...frogs, mice, and even humans. It binds to a specific set of DNA-binding transcription factors to repress... from. Let’s take a look at some of the stories behind the names. We also have many plasmids in our repository...Addgene has several plasmids that contain SHH, you can find them all here! The most popular SHH plasmid however...terrible fate when she pricked her finger on the spindle of a spinning wheel but also a biological transposon...vegetable substitute for spaghetti? I actually couldn’t find the specific origins of the name but some of our...properly (Azplazu and Frasch, 1993). I could not find the exact origin of this gene name but the larval...Plasmids 101 blog series Resources on Addgene.org Find plasmids for your research Deposit plasmids at Addgene...

...fused to a degron, a domain that induces degradation. In plants, auxin binds to TIR1 of the SCF-TIR1 ubiquitin...it’s briefer time scale and its inducible and reversible nature. Find the LADL plasmids at Addgene Kim...deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like...Article contributed by Alyssa Cecchetelli Auxin Inducible Degradation (AID) uses plant hormones called auxins... the AID technology (Yesbolatova et al., 2019). Find the mAID tagging toolkit at Addgene Yesbolatova ...specific PAM (protospacer adjacent motif) in order to bind the target sites for editing. PAM sequences can ...they also fused the SpCas9-NG with the activation-induced cytidine deaminase  Target-AID-NG, which mediated...

...voltage indicator Archon Article contributed by Shreya Vedantam  Listen to the voltage indicator podcast... genetically encoded voltage indicators. To expand the voltage indicator toolbox, Ed Boyden’s lab developed...small scaffold proteins to identify novel binding proteins that bind to either the light- and dark- states...cPYP), and plasmids that encode the protein binders (which bind to either the light- or dark- state of the..., because cPYP binds to BoPD. A similar set of newly identified light- and dark- binder plasmid tools ...for the photoswitchable AsLOV2. Find the cPYP and AsLOV2 protein binder plasmids here! Reis JM, et al. ...deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like...

...the ribosome binding site is inaccessible. When the target RNA is present, however, it binds to the switch...deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like...the dsRNA leading to increased RNAi efficiency. Find the T444T plasmid here! Strum A, et al. Nucleic ...an option, including Drosophila and C. elegans.   Find the TurboID plasmids here! Branon et al. Nat Biotechnol...switch and exposes its ribosome binding site, allowing for the gene on the toehold switch to be expressed...display and deep sequencing, revealing that they bind to a much larger subset of protein motifs than previously...biophysical study involving protein stability or binding experiments. Potential experiments that one might...

...publically available through Addgene. Stovicek, et al. J Ind Microbiol Biotechnol. 2015. PubMed PMID: 26376869...Toolkit Many industries, such as the food and beverage industry, the bioethanol industry, and the pharmaceutical... make it easier for you to find and use the plasmids you need. You can find all the hot plasmids from ...Nile Virus (WNV) induced cell death. The Wu lab library consists of 77,406 individual gRNAs targeting ...optimizations are required for each individual target species. Indeed, in order to use MAGE, the λ Red recombinase...for genetic optimization of industrial yeast strains. However, industrial yeast strains are more difficult...Targets of RNA-binding proteins identified by editing Identifying targets of RNA-binding proteins (RBPs...

...FKBP rapamycin binding domain of mTor (Cas9(N)-FRB-NES) resulting in a rapamycin-inducible Cas9 for genome...Rapamycin-inducible Cas9 sets (Addgene plasmids 62883 &62884; 62885 & 62886) Rapamycin-inducible dCas9-VP64...domain unfolds allowing it to bind to SspB. This original Light Inducible Dimer (oLID) could be used to... not show large changes in binding affinity with light stimulation. Indeed oLID showed only a two-fold...throughput binding assays, to engineer photoswitchable dimers which show over a 50 fold change in binding affinity... coat protein binding sites and 24 copies of the MS2 bacteriophage coat protein binding sites to tether...constitutive or inducible, gene knockdown with shRNA or shRNA-miR30, (2) tamoxifen-inducible CreERT2 gene...

...ON. When a repressor binds the activator protein, the activator protein cannot bind the promoter sequence... cannot bind the promoter sequence and transcription is ON. Once a co-repressor protein binds the repressor...derivatives is added, it binds tTA, resulting in a confirmation change that prevents binding to the promoter and...cumate is added, it binds cTA, resulting in a confirmation change that prevents binding to the promoter and.... As ethanol accumulates, it binds to the repressor, enabling it to bind the promoter and repress pADH1...system). The GAL80 repressor can bind to GAL4, partially inhibiting the binding of GAL4 to UAS. One can therefore...manner, with LexA binding to the lexA operator (lexAop). Fusing the LexA DNA-binding domain (DBD) to GAL4...

...interactions. Next comes the binding step where the target antigen binds to antigen-specific antibodies...conjugated antibody binds to antigen coated on the multiwell plate. In an indirect ELISA a primary antibody...antibody first binds to the antigen and then a reporter enzyme conjugated secondary antibody binds to the primary...antibody that binds to the antigen in a sample and then a second detection antibody binds. The detection...less antibody available to bind to the competing antigen. In direct, indirect and sandwich ELISA the reporter...research before conjugating at the bench.   Indirect ELISAs The indirect ELISA process is similar to that of ... easy to find,  it is often easier for labs to source appropriate antibodies for an indirect ELISA than...

...dark-induced dark-induced dark-induced Loop 6 blue-light induced dark-induced blue-light induced Table...controlled binding, but with opposite effects: insertion into loop 1 caused dark-induced binding while loop...OptoNBs with dark-induced binding, but insertion into loop 6 generated both blue light inducible and dark-inducible...OptoNB can bind, it co-localizes with its target at the cell membrane and when the OptoNB can’t bind, it localizes...loop 6 insertion caused blue-light induced binding. The lab used this general design scheme to generate...the effect of AsLOV2 insertion site on light-induced binding.  Light controlled protein purification with... path to light-controlled protein binding, they probably don’t bind your favorite protein yet. More OptoNBs...

...While a primary antibody binds to the protein of interest, a secondary antibody binds to the primary antibody...primary antibody, due to increased binding affinity, many indirect antibody protocols are designed to ...anti-mouse secondary will bind to all the primary antibodies indiscriminately, giving them the same output...antibody with a signaling molecule, the antibody binds to a protein, and then voila! Wherever your protein...protein of interest, it may be time to look at the indirect approach - which means it’s time to talk about...antibody. In the indirect approach, the signaling molecule is conjugated to the secondary antibody instead...antibody. Since multiple secondary antibodies can bind to a single primary antibody, this approach can ...