...determine if proteins are targeted to their destination co- or post-translationally. TRICK: Visualizing the...first round of translation While ribosome profiling co-opts the physical location of the ribosome, TRICK...

...all organisms and cell types, (ii) no need to add a co-factor, (iii) easy visualization with standard filter...conditions, proteins that bind endogenous fluorescent co-factors can be used. Examples include the LOV domains...Kumagai, 2013). Next to these naturally occurring co-factor binding proteins, systems wherein a protein...

...and Women’s Hospital (BWH). Based on her experience co-directing Mentor Circle Programs at BWH and providing...is a Junior Faculty at Harvard Medical School/BWH, co-Director of the Mentor Circle Program at BWH, and...health strategy, and global health equity. She is also Co-founder/director of the non-profit foundation IMFAHE...

...for methanol usage. This gene promoter, AOX1, was co-opted for the expression of recombinant proteins ...Saccharomyces cerevisiae to human: The important gene co‑expression modules. Biomedical Reports 7.2 (2017)...

...fluorescent protein tag which let the team detect its co-localization with a membrane-localized mCherry or...protein. With this system, if the OptoNB can bind, it co-localizes with its target at the cell membrane and...

...trans-protein splicing. Note, however, that this will require co-delivery of two AAVs at a high MOI, often resulting...microglia in vivo.   Q5: What is the efficiency of co-delivering different AAV particles into the same ...

...Concentation*** Blasticidin bsd  HeLa, NIH3T3, CHO, COS-1, 293HEK Inhibits termination step of translation...termination 1-10 ug/mL Zeocin Sh bla HeLa, NIH3T3, CHO, COS-1, 293HEK, Jurkat T cells Complexes with DNA; causes...

...number of new platforms (such as openUC2, which I am a co-founder of), that aim to support the community in...the Heintzmann Lab at the Leibniz IPHT Jena. He is co-founder of the open-hardware startup “openUC2” and...

... with its variable chain domain. B cells start by co-expressing IgM and IgD antibodies, but later in an...recombination of the antibody gene locus. B cells start by co-expressing IgM and IgD isotypes antibodies which ...

...postdoctoral researcher at the Noble Research Institute, co-instructed the ASPB workshop and authored a three-part...Global Community Bio Summit at the MIT Media Lab. As a co-founder of a community lab, I realized that not all...

...et al, 2012, Bindels et al, 2017). The plasmid for co-expressing mTurquoise2 with mVenus as a reference...this data. The practical brightness assay (employing co-expressing fluorescent proteins or using endogenous...

...ClonTracer library originally came about as Bhang and co-workers were trying to develop a way to quantify ...ClonTracer to study drug resistance Although Bhang and co-workers started using the tool to look at a variety...

...steady-state complexes with secondary coregulator (or co-coregulator) partners. The coregulator ‘core’ complex...group of proteins, whereas the more loosely bound co-coregulators associate with the core complex in a...

...promoter sequence and transcription is ON. Once a co-repressor protein binds the repressor protein, the...promoter is less leaky than UAS in the basal state, and co-expression of the inducer QF can increase QUAS driven...human HeLa cells as compared to basal expression. Co-expression of repressor QS along with QF leads to...

...independently packaged into an AAV. When a cell is co-infected with both part A and part B AAVs, the fragments...of these variables influence whether cells will be co-transduced with multiple copies of both vectors. ...and “part B” of the transgene. These two AAVs are co-transduced in a single step and serve as donors for...

...means spending more time with our pets. These new “co-workers” have made their way onto our Slack workspace...

...viruses to express genes or gene sets over 4.7 kb. Co-infection rates for AAVs are generally high enough...packaged in them and use elements such as IRES or 2A to co-express them from one promoter. Specificity The ...

...to do more than just label cells. If GFP could be co-opted to control gene expression, he could then selectively...-binding proteins (GBPs) to find those that could co-occupy GFP. Once they’d found suitable pairs, they...

...  Level of co-expression among cellular markers You’ll also need to know if markers are co-expressed in...

...that bind an E3 ligase. These bifunctional degraders co-opt E3 ligases such as cereblon (CRBN) or von Hippel-Lindau...refer to as the degradation tag (dTAG) system, to co-opt the endogenous ubiquitin proteasome machinery...