Skip to main content
Addgene
Showing: 21 - 40 of 115 results

  1. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ...updated CRISPR 101 e-book (to help with the other 11 presents!)  Can't get enough of CRISPR? Neither can...

  2. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...with gene editing. CRISPRainbow requires very short, 11-mer gRNAs, which do not induce genome editing. If...editing, activation and repression.” Nat Methods. 12(11) (2015):1051-4. PubMed PMID: 26344044. PubMed Central...

  3. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...site: TNA(C/G)TTATAA(G/C)TNA. Tol2 can deliver 10-11 kB to mammalian cells without decreased efficiency...payload for lentivirus). Large cargos, such as the ~11 kB cDNA for dystrophin, the gene mutated in muscular...Transposons: Nature and Applications in Genomics. CG 11:115–128. https://doi.org/10.2174/138920210790886871...

  5. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...Ten-fold Robust Expansion Microscopy (TREx). Elife 11:e73775. https://doi.org/10.7554/elife.73775  Dani...proteins in their ultrastructural context. Nat Commun 11:3850. https://doi.org/10.1038/s41467-020-17523-8 ...

  6. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...coefficient of a protein. Protein Sci. 1995 Nov;4(11):2411-23. doi: 10.1002/pro.5560041120. PMID: 8563639...particle formation. J Pharm Sci. 2015 Feb;104(2):602-11. doi: 10.1002/jps.24259. Epub 2014 Nov 21. PMID: ...

  7. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...high generalization performance. Science Advances, 5(11), eaax9249. https://doi.org/10.1126/sciadv.aax9249...repair determinants. Nature Biotechnology 2023, 1–11. https://doi.org/10.1038/s41587-023-01678-y Li, X...

  9. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...Nguyen M, Trono D, Naldini L. J Virol. 1998 Nov;72(11):8463-71. PubMed PMID: 9765382. PubMed Central PMCID...Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. PubMed PMID: 25075903. PubMed Central PMCID...

  10. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...proteins. Our studies and those of others (5, 6, 7, 10, 11) highlight the very real consequences of misfolded... PMID:15557121. Pubmed Central PMCID: PMC2172584. 11. Jokitalo, Eija, et al. "Golgi clusters and vesicles...

  11. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...produce Murray’s clear solution.  Murray's clear (11) was mostly used to study the development of vertebrate...M. Sheng, H.-U. Dodt. (2012). Nature Protocols, 7(11), 1983–1995. Pubmed. K. Chung, J. Wallace, S.Y. Kim...

  13. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ... Cerevisiae.” ACS Synthetic Biology, vol. 12, no. 11, American Chemical Society (ACS), 6 Nov. 2023, pp...Toolbox for Plants.” ACS Synthetic Biology, vol. 3, no. 11, American Chemical Society (ACS), 20 Feb. 2014, pp...Mammalian Cells.” ACS Synthetic Biology, vol. 8, no. 11, American Chemical Society (ACS), 5 Nov. 2019, pp...

  14. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...not include stem loop 3, and guide RNA nucleotides 11-20 are not resolved due to structural disorder. HNH...DNA target are essential for cleavage. Nucleotides 11-20 are disordered and don’t appear in the Figure ... editing enzymes. Nature Reviews Microbiology, 20(11), 641–656. https://doi.org/10.1038/s41579-022-00739...

  15. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...from researchers at Amgen, it was stated that only 11% of the academic research in the literature was reproducible...specific compliance requirements – e.g. 21 CFR part 11, HIPAA? Answering this question helps you evaluate...

  16. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...Derived Transcriptional Activator Systems. PLoS ONE 11:e0158294 . https://doi.org/10.1371/journal.pone.0158294...control of endogenous gene activation. Nat Chem Biol 11:198–200 . https://doi.org/10.1038/nchembio.1753 Tanenbaum...

  17. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ... National Academy of Sciences 110.18 (2013). 7306-11. Pubmed PMID: 23589850. 9. Cronin, J., Zhang, X.Y...PMID: 22407723. PubMed Central PMCID: PMC3456965. 11. Zhang B., Metharom P., Jullie H., Ellem K.A., Cleghorn...

  20. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ... for multicolor imaging across scales. Nat Commun 11, 239 (2020). Oliinyk, O.S., Shemetov, A.A., Pletnev...dynamics of protein-protein interactions. Nat Methods 11, 641-644 (2014). Filonov, G.S. & Verkhusha, V.V. ...
Showing: 21 - 40 of 115 results