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We narrowed to 149 results for: Coli

Showing: 51 - 100 of 149 results

  1. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...acids E. coli and Mammalian Cells pEVOL-pAzF synthetase for p-azido-l-phenylalanine E. coli pEVOL-...proven most effective only in a few cell types (E. coli and Xenopus oocytes, mainly). Nevertheless, tRNA...pEVOL-pBpF synthetase for p-benzoyl-l-phenylalanine E. coli pAcBac1.tR4-MbPyl pyrrolysyl-tRNA synthetase Mammalian...

  2. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    Published
    Dec. 2, 2019, 2 p.m.
    ...Burke Lab (Roux et al., 2012). BioID utilizes the E. coli biotin ligase, BirA*, with a site mutation (R11G...DNA-binding domain (233 amino acids) found in the E. coli version. It also contains a single mutation (R40G... noise ratio 30-fold compared to the standard E. coli BirA* mutant. RapID consists of this mutant BirA...

  3. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...gram negative bacteria include salmonella and E. coli. Because of their thicker peptidoglycan layer, gram... Blue-white screeningPlasmids 101: Common lab E. Coli strains  ...

  4. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...reaction into chemically or electro-competent E. coli - excess salt causes arcing during electroporation...vaccinia virus DNA topoisomerase I in Escherichia coli is sequence specific."  Proc Natl Acad Sci U S A...

  5. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...overexpression of various metabolites in Escherichia coli or Saccharomyces cerevisiae with few techniques ...metabolic engineering realm of S. cerevisiae and E. coli. The utility of simultaneous and multidirectional...

  6. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ... that’s easier to express in the cytoplasm of E. coli, although this is not possible for all scFvs.   ...single-chain Fv analogue produced in Escherichia coli. Proceedings of the National Academy of Sciences...Sivaccumar JP, Ruvo M (2020) Evolution of Escherichia coli Expression System in Producing Antibody Recombinant...

  8. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...polymerase I (PolI3M) is capable of mutagenesis in E. Coli, but this approach requires special bacterial growth...mutations per nucleotide per generation of wild-type E. coli, while only increasing the standard mutation rate...

  10. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...nonmethylated PCR products are then transformed into E. coli to undergo nick repair. This method can work well...relying on in vitro assembly, they could make the E. coli do the work by using the principles of recombineering...

  11. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...opposite, a uracil DNA N-glycosylase (UNG) from E. coli. The resulting construct, eUNG-BE4max(R33A)ΔUGI,... of 30 target sites. But the same strategy in E. coli produced different results. GBEs seemed to follow... by excising the freshly edited U bases — but E. coli instead filled those abasic sites with A's, producing.... In addition, they showed that UNG from both E. coli and C. elegans could be effective components of ...

  12. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ... With MAGIC, scientists introduce an Escherichia coli donor strain into the microbiome to deliver a genetic.... The lab used DCDC to measure growth rate of E. coli for over 10 generations and showed that this method...species such as Snodgrassella alvi and Gilliamella apicoli. The plasmids could also be transformed into Alphaproteobacteira...

  15. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...plasmid multimerization in the naturally occurring E. coli plasmid ColE1 (Goebel & Helinski, 1968; Bazaral ...Bedbrook & Ausubel, 1976; Fishel et al., 1981). E. coli strains such as DH5α, HB101, NEB Stable, Stbl3, ...harboring large inserts are often dimers in Escherichia coli K-12. Plasmid. 1989 Mar;21(2):138-41. doi: 10.1016...Characterization of multiple circular DNA forms of colicinogenic factor E-1 from Proteus mirabilis. Biochemistry...

  17. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    Published
    June 13, 2016, 7:08 p.m.
    ...addgene.org. If you are looking for plasmids used in E. coli and yeast, you’re in luck! Addgene has so many of...

  18. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...were used to express the necessary components in E. coli (Klompe et al., 2019). Recently, the lab introduced...temperatures. Data adapted from Vo et al., 2020. In E. coli, the new streamlined VchINT system was shown to ...

  19. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...with codon bias across all endogenous genes in E. coli and S. cerevisiae (Tuller et. al., 2010). ...codon optimizing a human gene for expression in E. coli. Genes are also optimized for expression in mammalian...

  20. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...from the bacterial genome, and inserted it into E. coli to express and then test for any proteins that could...addition, when they looked at the transformed E. coli expressing Cas7-11, they found no evidence of growth...

  21. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...described by studies of EcoRI and Bsp6I in Escherichia coli in 1995 when introduction of restriction-modification...Engelberg-Kulka, and Gad Glaser. "An Escherichia coli chromosomal" addiction module" regulated by guanosine...

  22. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...supplemental add-on kit and is compatible with E. coli and/or cell-free systems. Protein expression in ...al. “EcoFlex: A Multifunctional MoClo Kit for E. Coli Synthetic Biology.” ACS Synthetic Biology, vol. ...MoClo: Improved MoClo Assembly Standard and New E. Coli Part Library Enable Rapid Combinatorial Design for...

  27. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...simplified the process of generating multiple E. coli gene expression plasmids with the Cross-disciplinary... cloning that results in the creation of many E. coli constructs with greatly varied levels of expression...endeavours of unparalleled complexity in Escherichia coli, like the construction of a so-called “genomically... as a dominant-negative mutator allele of the E. coli MMR protein MutL, all under the control of the cI857...MutL is highly conserved in distant relatives of E. coli so it can be used in a broad range of strains. Thus...but these domains often do not express well in E. coli without their partner regulatory domains. The Chodera...

  28. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ... E. coli tRNA adenosine deaminases (ecTadA), fused it with dCas9, and transformed it into E. coli harboring...

  29. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...et al. "Expanding the genetic code of Escherichia coli with phosphoserine." Science 333.6046 (2011): 1151...flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation." Nature...

  31. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ...to make copies of the newspaper, Addgene uses E. coli bacteria to generate copies of plasmids. The community...

  32. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...phosphorylated amino acid to the genetic code of E. coli (Park et al., Science 2011). The Rinehart lab has...methodology in two differents types of bacteria (E. coli and Salmonella typhimurium) by integrating DNA sequences...expression and purification of Cas9 protein in Rosetta E. coli. New Lentiviral CRISPR activator and repressor plasmids...constructs constrained only by the ability of E. coli to maintain the final plasmid after transformation...

  36. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...proteins. She also showed that R-bodies can rupture E. coli membranes. Read the full post for potential applications...

  38. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...thermophilus DNA ligase, a homolog of Escherichia coli DNA ligase." Journal of bacteriology 173.16 (1991...Last stop on the road to repair: structure of E. coli DNA ligase bound to nicked DNA-adenylate." Molecular...

  41. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...distribute your bacterial strains if they: are E. coli that can be used under BSL1 conditions were generated...you to produce large amounts of your insert in E. coli, then this is a Bacterial Expression vector. If ...

  42. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...Cre-lox Light-Switchable Two-Component Systems for E. coli Browse other blog posts from our Plasmids 101 series...

  44. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...Standards Synthetic Photobiology: Optogentics for E. coli Browse All Synthetic Biology Blog Posts Resources...

  46. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...20, 30, 40, 50, 60, 80 and 100 kD proteins in E. coli, either individually (~20,000 lanes/50 mLs) or through...
Showing: 51 - 100 of 149 results