...10 Ways to Share Your Science 6 Tools & Tips: Online Social Networking for Scientists Sharing Your Lab ...on the AMA process below, but we also wanted to share some thoughts on why we decided to do an AMA in ...reasons why you should consider using Reddit to share your science. While Reddit isn’t for everyone, particularly...well moderated. One of our original worries about sharing science advice on Reddit was that we’d be dragged...which falls in line with our general mission to share- as opposed to sell- science. 4. You can prepare... Before I turn it over to Joanne, I thought I’d share one of my favorite questions from the AMA - as you...Experiments Additional Resources See How We're Sharing Science through Video with the Addgene Youtube ...

...interest in the ways science is shared and in making it easier to share, we take special note as the open... to Share Science, in honor of a decade spent making plasmids more accessible and easier to share. As ...those of us at Addgene believe in the power of sharing, and especially in keeping the products of science...let's make a toast to open science. And, please, share your favorite OA developments of the past year with... comments. Or let us know your favorite ways to share science. Image credit: Judy Breck, via flickr ...

...thought leader in scientific sharing, leading by example to increase sharing in the community Addgene fosters...Ramsey, Randall, Ray, Rebecca, Renee, Shannon, Susanna, Tenisha, Thomas, Tony, Tracy, Tyler, Tyrel, Wogenie...transparency. We don’t have to explain that the materials shared through Addgene are being used for research purposes...we attract partnerships with organizations that share our community-oriented values. Addgenies like the...our mission to accelerate research with easier sharing and collaboration. Joanne and all the Addgenies...

...data if you need to. Similarly, FigShare also makes it easier to share data through their curation of ... at Harvard University 29:31 - 49:45 - Reagent Sharing - Susanna Bachle, Addgene the nonprofit plasmid...working to provide tools that make it easier to share in-depth information about reagents, protocols, ... Companies like Code Ocean allow researchers to share code they’ve used for data analysis while other ...organizations like Dryad make it easier for researchers to share data thereby enabling you to do modified analyses...science Twitter is very active with many scientists sharing their unpublished data. There are blogs - both ...ReFigure: Save Scientific Figures into Dashboards and Share Your Insights Who gives a tweet? 9 facts about scientists...

...Before I started writing for the Addgene blog, sharing Chemistry Cat memes was how I used social media...professionally, and 2) I needed to start using Twitter. Sharing Chemistry Cat memes use to be how I used social...used colony PCR before and thought it was cool to share my knowledge with other scientists who maybe hadn...paper. I wrote an Addgene blog post about it and shared the paper with my lab since one of the AAV vectors...of the #BLACKandSTEM Twitter community. Dr. Page shares her story of how one seemingly innocuous tweet ...the pointers are different from the other links shared in the post. And the blog writing tips are elements...media helped you advance your research or career? Share your story in the comments below!   Additional...

...to all of our generous, sharing depositors. Over 3,000 laboratories are sharing plasmids (with 16 labs ...labs currently sharing viral preps) through the Addgene repository. Without these scientists and the support...to have such an impact on scientific materials sharing. I would also like to recognize a few scientists...Addgene has been successful in helping thousands of sharing scientists from laboratories all over the world...the scientists were waiting. I look forward to sharing new developments on this project and on all the...the interesting things going on at Addgene. Sharing speeds science and we are proud to be adding viral ...

...expression stable regardless of copy number (Segall-Shapiro et al., 2018). Designing stabilized promoters To...the case of the stabilized promoters from Segall-Shapiro et al., they chose a TALE protein as the repressor...replication, the stabilized promoters created by Segall-Shapiro et al. showed nearly no position-dependent differences...division slows (Kittleson et al., 2011). Segall-Shapiro et al. showed that the stabilized promoters were...during the transition to stationary phase, Segall-Shapiro et al. placed a trans-acting RepA protein under... proteins are needed sequentially. When Segall-Shapiro et al. placed the genes under the control of a ...stabilized promoters a try. References 1. Segall-Shapiro, Thomas H., Eduardo D. Sontag, and Christopher ...

...exchange vectors here! ShadowR, a novel FLIM-FRET acceptor Figure 4: ShadowR is used as a FRET acceptor...or mScarlet. Image: Murakoshi et al., 2019. ShadowR is an orange light-absorbing chromoprotein that...FLIM-FRET) in living cells (Figure 4). They created ShadowR by modifying the surface of the chromoprotein Ultramarine...binding to proteins can inhibit their activity. ShadowR can be used as a FRET acceptor in combination with...or mScarlet as the donor in HeLa cells.   Find ShadowR for mammalian cell expression here   Now that you...M., Sato, A., Koga, N., & Nabekura, J. (2019). ShadowR: a novel chromoprotein with reduced non-specific...

...., Gore, B. B., Weed, N., Omstead, V., Bishaw, Y., Shapovalova, N. V., Martinez, R. A., Fong, O., Yao,...expressed in both BALB/cJ mice and C57BL/6J mice (Shay et al., 2023; Huang et al., 2023). Systemic capsids...CNS: neurons and astrocytes Mice No (LY6C1) Shay et al., 2023 AAV-PHP.V1* CNS: Biased toward...cells in the CNS of both BALB/cJ and C57BL/6J mice (Shay et al., 2023). AAV-PHP.AX is biased toward astrocytes...would also like to thank Viviana Gradinaru and Tim Shay for their support and input. Resources and references...Bindu, D. S., Zhang, M., Taskin, N., Goertsen, D., Shay, T. F., Sullivan, E. E., Huang, S.-F., Ravindra ...X., Lefevre, A., Singh, V. P., Arokiaraj, C. M., Shay, T. F., Vendemiatti, J., Jang, M. J., … Gradinaru...

...include materials sharing in their dissemination plans and in particular, that they all share the plasmids ...scientists from 21 different labs who clearly also share this wonder of the organism.   It’s not an overstatement...ways to culture, propagate, transform, store, and share all of these new organisms continues to be challenging...systems. How the EDGE Program promotes scientific sharing The EDGE Program is designed to expand the number...The parasitic fusion is such that the two plants share RNA, proteins, and DNA. Euprymna scolopes (Joshua...re excited to see new tools being developed and shared for many different organisms. Do you have a favorite...

... what challenges remain in materials sharing, information sharing, and research reproducibility while ...to reflect on our accomplishments from 2022 and share what we’re looking forward to in 2023 and beyond...2022 we also rolled out an updated AAV Data Hub to share curated information about AAV usage in research ...around the world can upload AAV validation data, sharing what works and, equally importantly, what doesn...commitment to supporting the research community through sharing science and distributing high-quality materials...

...resources References Shemetov, A.A., Oliinyk, O.S. & Verkhusha, V.V. How to Increase Brightness of Near-Infrared...4557 (2017). Shcherbakova, D.M., Baloban, M. & Verkhusha, V.V. Near-infrared fluorescent proteins engineered...Shcherbakova, D.M., Stepanenko, O.V., Turoverov, K.K. & Verkhusha, V.V. Near-Infrared Fluorescent Proteins: Multiplexing...Shemetov, A.A., Pletnev, S., Shcherbakova, D.M. & Verkhusha, V.V. Smallest near-infrared fluorescent protein...Nat Methods 11, 641-644 (2014). Filonov, G.S. & Verkhusha, V.V. A near-infrared BiFC reporter for in vivo...Shcherbakova, D.M., Cox Cammer, N., Huisman, T.M., Verkhusha, V.V. & Hodgson, L. Direct multiplex imaging and...Nat Commun 5, 3626 (2014). Shcherbakova, D.M. & Verkhusha, V.V. Near-infrared fluorescent proteins for multicolor...

...openness and sharing in science.  We’re excited to support the neuroscience community by sharing not only ...neuronal manipulation. The goal of the workshop was to share the results of these discussions as a publicly accessible...To complement these efforts, we’re beginning to share data through our recently launched AAV Data Hub,...researchers using AAV from our viral service can share experimental design and data. With these different...science, we aim to accelerate the rate by which sharing speeds science.   Check out the precise neuronal...

...in Dr. Zhang's introduction. His willingness to share reagents so freely with the academic community has...a puzzle to decrypt to a tool to be harnessed. Sharing CRISPR, synbio, & more via Addgene Addgene's addition...repository, and I was proud to be responsible for sharing some of our data at this meeting. We weren't quite...gratitude for the role Addgene plays in plasmid sharing to those who were anxious to hear about what was...have given the attendees an amazing opportunity to share their cutting edge science in increasingly important...Interested in Addgene's Keystone Poster?  We'll be sharing the poster with our blog readers and on social ...

...very anxious at this point. Here's the message I shared with the Addgenies on March 11, 2020 after rumors...This is a time when we need as much open science sharing as we can get. The effects of Addgene’s brief...for grant-driven research being done in all labs sharing materials via Addgene. Perhaps if each of many ... unique resource for scientists that helps them share reagents — leading to open, reproducible science...pandemic-related slowdown. More scientists are sharing plasmids and other reagents every day, and this..., and we look forward to supporting scientific sharing of materials for the foreseeable future. ...

...research in terms of preparedness, organization, and sharing data. Here are five FREE science lab apps that ...is a multipurpose application that allows you to share your data with colleagues and link your notebook...the information about that restriction enzyme or share it via email with a colleague. The app is also great... Quiz/Card by Andrey Solovyev Lab Help by Kris Shamloo These are just a few of the many apps available...the lab. If you have a favorite lab app, please share it in the comments section below! Many thanks to...Notebook for Bioinformatic Analyses Use Apps to Share Your Lab Protocols Check Out How Addgene Develops...

...for supporting scientific sharing. We’re very excited for more reagent sharing projects in the years to...15 years of plasmid sharing has certainly taken us on many adventures...From moving office locations ...service in 2016...we’re excited to help scientists share their reagents with the scientific community.   ... tools. How many times have these plasmids been shared in total? Just over 49,000 times! Their long-lasting... from the Zhang lab. In fact, the Zhang lab has shared over 40,000 CRISPR-Cas9 components over the years...

...presentations and a handout on protocol sharing, material sharing, computational reproducibility, and tools...wet lab protocols, reagent sharing, bioinformatic tools, data and code sharing, and data analysis and visualization...who were also thinking about reproducibility and shared similar desires to take action. Thanks to Teytelman...s contributions by Tyler Ford included reagent sharing and were presented at the ASPB meeting by the collaborators...

...sequences for antibodies are not shared. For instance, through open plasmid sharing, the CRISPR community has...wanted to hear a piece of music. It was tough to share or remix the songs, the CDs could get scratched ...antibody technology makes it easy for scientists to share, remix, store, and track these essential components...perpetuity.  Second, plasmids are easy to store and share, making recombinant antibodies the most practical...10.1083/jcb.201409074 Tran NH, Rahman MZ, He L, Xin L, Shan B, Li M (2016) Complete De Novo Assembly of Monoclonal...

... holiday celebrations! We're kicking it off by sharing all the incredible guest posts and stories featured...others chose to work with Addgenie writers to help share their research, tools, and knowledge. Guest authors... Getting a Faculty Position Author: Erik Lee SnappSharing is Caring, But How to Distribute Open Hardware...Cynthia Arokiaraj; Viviana Gradinaru; Tim ShayMultifunctional Neuronal Fibers: Modulation from Gut to BrainResearchers...have helped researchers around the globe find and share knowledge, resources, and new ideas - we're so grateful...

...and a key step for that is sharing the data when an AAVs doesn't work. Sharing your negative data helps ...Addgene AAV Data Hub, the place for scientists to share their AAV validation data. The Data Hub contains...incredible AAV talks given by Connie Cepko and Ian Wickersham.     Submitting to the Data Hub If you've ever...others and, when published, will meet FAIR data-sharing guidelines. But what if my AAVs didn't work?  ...

..., developed and deposited at Addgene by Arlene Sharpe’s lab, allow for wide deployment of in vivo gene...Marty LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested...Montero Llopis, P., Haining, W. N., Doench, J. G., & Sharpe, A. H. (2024). X-CHIME enables combinatorial, inducible..., Al Abosy, R., Freeman, G.J., Haining, W.N., & Sharpe, A.H. (2019). PTPN2 regulates the generation of..., Coxe, D. J., Doench, J. G., Haining, W. N., & Sharpe, A. H. (2019). A CRISPR-Cas9 delivery system for...

...to quickly find what you’re looking for. Tip 3: Share protocols and spreadsheets with lab members Now ... software, there are many free and easy ways to share your documents with your colleagues. Start small...frequently. Copy these into an electronic file sharing tool, such as Google Drive or Dropbox, and file...file them into a folder that you plan to share. The programs will allow you to choose who should have access... stop back and let us know what worked for you. Share your best tips, tricks, and ideas below. ...

...from 47 different tissues. Researchers upload and share both their successes (recommended reagents) and ... with the recommendation. The goal is to easily share and find what works and what doesn’t while engaging...They particularly encourage people to upload and share their negative data. After all, they tell me, when...anyone who has used IBEX and wants to preserve and share their validation work. Everyone who contributes ...the Zenodo release in keeping with their value of shared ownership. Go check it out! References and Resources...

...community, sharing, and fun. The Addgene repository is built on the foundation of a community that shares reagents...and repeat - we’re proud and excited to finally share with you what we’ve been working on behind the scenes...much easier to get around our site.  Community, sharing, and fun Along with improving search and navigation..., fun, and promote our values of community and sharing.  One big feature upgrade? Blugene now has a full...

...and share user data on the antibody webpages. These outlets can be particularly useful for sharing negative...protein, allowing you to proceed with confidence.  Share your results Ultimately, like all of science, validation...validation experiments don’t mean anything unless you share the results with others. If you publish data generated...antibody’s trustworthiness. You may also consider sharing your validation attempts by other means, such as...antibody (perhaps one from Addgene!), consider sharing your results so that others in the community can...

...CRISPR systems can be as confusing as reading Shakespeare for the first time. The discovery and application...as it seems. There is not a single gene that is shared across all CRISPR types; the fast evolution of ...systems that are in the same class and type don’t share sequence similarity but are grouped due to mechanisms.... S., Wolf, Y. I., Alkhnbashi, O. S., Costa, F., Shah, S. A., Saunders, S. J., Barrangou, R., Brouns, ...Horvath, P., Moineau, S., Mojica, F. J. M., Scott, D., Shah, S. A., Siksnys, V., Terns, M. P., Venclovas, Č...., Garrett, R. A., Randau, L., Sørensen, S. J., & Shah, S. A. (2019). Type IV CRISPR–Cas systems are highly...

...about five years ago. I was interested in what she shared about the company and what the goals were and so... to the scientific community through Addgene’s sharing platform. Expanding further beyond the US and increasing...lawyers. While there will always be an interest in sharing, there will always be an interest in protecting...challenge.Paratore: The promise there is the ability share the information, making it available to everyone...efficiency through which knowledge is created and shared. Can you tell me a bit about your job? Katz: I’...

...Hodges Sharon Dent Archa Fox Elaine Fuchs Kai Ge Robert Kingston Thoru Pederson Phil Sharp Julie Ahringer...! And if you study chromatin and want to start sharing your plasmids through Addgene, don’t hesitate to...spanning 20 years of research. DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes.... 2015 Jan 15;29(2):197-211. DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes...

... we were closing in on the one million plasmids shared milestone in 2018, we wrote a post on the time ...plasmid. In 2023 (and at closer to 2 million plasmids shared than not!), we think it’s about time we updated... repositories Repositories such as Addgene help share materials quickly and cheaply. We save you from ... free for you, and many labs are quite happy to share, labs that get high volumes of plasmid requests ...cloning method Find blog posts about scientific sharing here Download the Plasmids 101 eBook Resources...

...lab has developed two new FLIM-FRET acceptors, ShadowG, a novel dark green fluorescent protein (GFP) with... with an absorption peak of 486 nm and ShadowY, a novel dark yellow fluorescent protein (YFP) with an ...fluorescence lifetime imaging microscopy) assays. ShadowG is darker than other dark YFP mutants, it reduces...desired due to its very low quantum efficiency. ShadowY has a lower quantum yield and higher absorption...reduced artifacts due to residual fluorescence. ShadowY was additionally shown to have a 7-fold lower quantum...

...unpublished data they may ask the audience to not share their research on social media. Use the hashtag ...live-tweeting any sessions that the presenter doesn’t mind sharing on social media. If you could not attend some of...as well. In addition to using social media for sharing new scientific information from the conference,...business,” but having business cards is a good way to share your information quickly. During the conference ...the Addgene blog Read other posts on scientific sharing, scientific communication, and scientific networking...

...perform functional tests. The newest genome engineer sharing the stage with much-lauded CRISPR-Cas9 is DNA-guided...difficulty with the NgAgo system. I encourage you to share your results and protocols with the research community...specifically set up a forum where NgAgo users can share protocols and tips Twitter - Tweet suggestions and...who have been lucky with NgAgo should explicitly share tips and protocol details with the scientific community...biotechnology (2016). PubMed PMID: 27136078. 2. Burgess, Shawn., et al. "Questions about NgAgo." Proteins & Cell...

...unrelated to the issue and consider how it could be shaped to solve your problem. In a study outlined in Originals... assure that all participants feel comfortable sharing their ideas? And how do we give people the opportunity...s advocate is elected, others may feel they can share opposing opinions as well. I am sure we have all...time. Meetings shouldn’t be the only place people share ideas. Ideas had over lunch are just as valuable... The purpose of this form was to encourage the sharing of ideas, not to give people more work. Identify...

...have been shared over 140,000 times to over 75 countries. That’s a lot of CRISPR! As we’ve shared these tools.... This week, we’ll continue to grow the ways we share science by hosting our first ever CRISPR Week on...think so we’ll ask you some questions on Friday - share with us how you use CRISPR. Hope you will join us...

...neuronal connectivity in the mammalian brain (Wickersham et al., 2007; plasmids at Addgene). AAV vectors...al., 2017), and these two modes of transport may share common mechanisms. Rare observations like these ...2014). Adeno-associated virus serotypes 1, 8 and 9 share conserved mechanisms for anterograde and retrograde...Frank, D., Kajikawa, K., Mise, N., Obata, Y., Wickersham, I.R., and Tonegawa S. (2014). Cell type-specific...Tanaka, M., Kearney, M., Chattree, G., Yang, C. F., Shah, N.M., and Mooney, R. (2017). Identification of ...Neurosci Res 93, 144-157. PubMed PMID: 25240284. Wickersham, I.R., Lyon, D.C., Barnard, R.J.O., Mori, T.,...

...The best part is, we’re not only sharing them with you, but you can share them with your coworkers, labmates...labmates, and friends as well!   Addgene is sharing so much more than plasmids these days. via GIPHY...out everything we’ve uploaded so far, and start sharing the science love today!        ...

... always, a big thank you to our depositors for sharing your science with us! Corralling and Cornering ...antibodies for use in mice and human brain IHC and sharing the data on the mAb3D atlas website.  The iGluSnFR3...Abudayyeh and Jonathan Gootenberg worked with us to share their new CRISPR tools, Cas7-11, a low-toxicity ...work!) If you've got a research story you want to share on our blog, please reach out to [email protected]...

... post on Instagram                     A post shared by Xi Chen (@seleena78) The lab has officially... post on Instagram                     A post shared by 草方 (@gracie_fang) That’s right, researchers...need only decorate your lab, take photos, and then share them on Twitter or Instagram with #DeckTheLab and... post on Instagram                     A post shared by Xi Chen (@seleena78)   Here’s how to enter:...

... during funding transitions. Enabling resource sharing: Our operating model allows researchers to access...strengthening our commitment to collaboration and resource sharing, we can navigate this difficult period together... Want to Hear from You We invite researchers to share how these funding changes are impacting your work...Addgene during this time. Your input will directly shape our response initiatives as we stand with the scientific...