... of Rogue Bioethics. On November 25, the MIT Technology Review dropped a bombshell report. A scientist... is unethical to use scientifically unproven technologies to edit a human embryo, or have a woman carry...controversial and ethically fraught rapidly emerging technology. We could do this for CRISPR/Cas9 – or any other...germline editing, we will initially limit the technology to research centers engaged in long-term, mandatory...review and decide on the future of genome editing technology. We must stop punting decisions on scientific... inform her work on emerging issues in novel technologies, including self-driving vehicles, synthetic ...

...treatment can lyse cells. Alcohols, like ethanol or methanol, fix and permeabilize in a single step by...sample (1). When combined with cell sorters, this technology can be used to isolate a specific subset of cells...and further analyze that subset of cells. An immunologist, for example, might be interested in the lymphocytes...cell into distinct populations (Figure 2). An immunologist who studies B cells, might then gate the B cell... Cytometry: An Overview. Current Protocols in Immunology 120: . https://doi.org/10.1002/cpim.40 Picot ...retrospective, fundamentals and recent instrumentation. Cytotechnology 64:109–130 . https://doi.org/10.1007/s10616...

...rapidly decode the complexity? The CombiGEM-CRISPR technology was developed with the goal of providing an easy-to-use...This blog post will introduce you to this new technology, and guide you through the basics of CombiGEM-CRISPR...particularly interested in developing and applying new technologies to interrogate and understand complex biological... off-target effects of CRISPR-Cas9." Nature biotechnology (2016). PubMed PMID: 26780180. PubMed Central...lentiviral CRISPR-guide RNA library." Nature biotechnology 32.3 (2014): 267-273. PubMed PMID: 24535568....combinatorial genetics in human cells." Nature biotechnology (2015). PubMed PMID: 26280411. PubMed Central...

...you want to read more content related to CRISPR technology, subscribe to our CRISPR blog content.   In this... dCas12f to VP64. Read the paper in Nature Biotechnology Find the Cas12f-GE plasmids Programmed genome...of target genes and cell types. Their Nature Biotechnology paper showcases an improved design of the guide...editing efficiency. Read the paper in Nature Biotechnology Find the epegRNA plasmids Enhanced prime editing... paired pegRNAs.  Read the paper in Nature Biotechnology Find the PRIME-del plasmids  Deletion and replacement...expression in the liver.  Read the paper in Nature Biotechnology Find the PEDAR plasmids   Additional resources...

...about Mammalian shRNA Tools for RNAi! Cre-Lox technology CAG-GFP-IRES-CRE (Plasmid #48201) This retroviral... and Cre-recombinase. The Cre-lox system is a technology that can be used to induce site-specific recombination...knockout, and selection marker removal. CRISPR technology pdCas9-humanized (Plasmid #44246) Expression ... the pLKO.1 vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid #17408)   This...lentiviral prep from the Addgene viral service. CRISPR technology lentiCRISPR v2 (Plasmid #52961) This is a 3rd...Maetzig T, et al. Gammaretroviral Vectors: Biology, Technology and Application. Viruses. 2011. PubMed PMID: ...

...University in Lisbon, Portugal. She leads the Glycoimmunology group based at the Research Unit on Applied...reported that PMM2-CDG patients suffer from immunological defects. These are characterized by a predisposition...play an essential role in almost every aspect of immunology, participating in events such as cell development... J., dos Reis Ferreira, V. & Videira, P. A. Immunological aspects of congenital disorders of glycosylation...winners! Paula Videira is the team leader of the Glycoimmunology group at UCIBIO, FCT-UNL, Portugal. Her main...role of glycans. Follow her on Facebook: @glycoimmunologygroup.     Michael McMurray is an Associate Professor...

... hood with a disinfectant (most people use 70% ethanol, but you can also use Incidin wipes) before and...everything that’ll be brought under the hood with 70% ethanol, including your glove-covered hands! You should...spills do occur, they should be wiped with 70% ethanol immediately. Finally, avoid sticking pipettes into...Most cell culture media contain the pH indicator phenol red (pH range 6.0 (yellow) - 8.0 (red)). Blood ...is particularly interested in Glycobiology and Immunology. You can find Sana on LinkedIn.   References ...et al. "Gene expression profiling analysis of bisphenol A-induced perturbation in biological processes...

... covers a brief review of different basic BE technologies and their adaptation for plant genome editing...State University Kutubuddin Molla is an Ag-biotechnologist in the National Rice Research Institute, Cuttack...CRISPR-Cas9 cytidine deaminase fusion." Nature biotechnology 35.5 (2017): 441. PubMed PMID: 28346401. 6. ...with a Cas9-cytidine deaminase fusion." Nature biotechnology 35.5 (2017): 438. PubMed PMID: 28244994. 7. ...fusion of nCas9 and human APOBEC3A." Nature biotechnology (2018). PubMed PMID: 30272679. 8. Hua, Kai, ...scope in rice by using Cas9 variants." Plant biotechnology journal (2018). PubMed PMID: 30051586. 9. Hua...

...group developed the original iteration of this technology in 2007. Used to label neurons in the cerebellum...cerebellum of mice, the team appropriately named their technology Brainbow. Brainbow 1.0 used the fluorophores ... promoter. Zebrabow is an extremely versatile technology as recombination can be induced in developing...cells. Tetbow optimizes Brainbow for current technologies Tetbow (Sakaguchi et al., 2018) overcame the...multiple fields of view, whereas previous Brainbow technologies were useful for discriminating neurons within... continue to develop new iterations of these technologies, expect to see systems that use newer, more ...

...hours of culture, when glucose is abundant and ethanol concentrations are low, pADH1 displays low promoter...activity of the strong yeast promoter pTEF). As ethanol accumulates, it binds to the repressor, enabling...engineered pADH1 variants that are less sensitive to ethanol, including the “middle” ADH1 promoter pADH1m, which... which maintains activity during the late ethanol consumption phase of yeast culture. Repressible Binary... regulated expression in mammalian cells. BMC Biotechnol 6, 43 (2006). https://doi.org/10.1186/1472-6750...expression system for Bacillus. Appl Microbiol Biotechnol. 2019 Jan;103(1):303-313. doi: 10.1007/s00253...

...wasn’t that easy. In the 1980s, gene targeting technology was invented to introduce specific changes into...into the mouse genome. With these early technologies, a researcher would first introduce a mutation into...transgenic offspring. Although powerful, this technology is cumbersome to use, not that efficient, often...first exposure to CRISPR when he used this new technology to engineer mouse models (Wang et al., 2013, ...others around the world. Yes, finally, we have a technology, CRISPR, that is simple in concept, straightforward...for CRISPR-Cas9–mediated gene inactivation. Nat Biotechnol 32:1262–1267 . https://doi.org/10.1038/nbt.3026...

...CRISPR and epigenetics As with many TALEN-based technologies, the advent of CRISPR has made targeting much...modification is certainly not the only CRISPR-based technology designed to alter gene expression. Fusing dCas9... genes from promoters and enhancers. Nature Biotechnology 33:510–517 . https://doi.org/10.1038/nbt.3199...regulatory elements in the human genome. Nature Biotechnology 35:561–568 . https://doi.org/10.1038/nbt.3853...programmable TALE-TET1 fusion proteins. Nature Biotechnology 31:1137–1142 . https://doi.org/10.1038/nbt.2726...modifications at endogenous enhancers. Nature Biotechnology 31:1133–1136 . https://doi.org/10.1038/nbt.2701...

...University of Science and Technology in Shijiazhuang, defended the technology in a news article in Nature..., NgAgo. As you might expect with any nascent technology, researchers are having trouble getting NgAgo...in this informative post. Interestingly, this technology may make some uses of Site Directed Mutagenesis...scientists. You can find all of our August posts in chronological order here: Site Directed Mutagenesis by PCR...

...who recently received a Ph.D. in Biology and Biotechnology from Worcester Polytechnic Institute. What is...With the meteoric rise and diversity of CRISPR technologies, one might incorrectly relegate RNAi to history...requires the presence of dsRNA. In plants, CRISPR technologies are predominantly used to disrupt function at... throughput survival screen found that either technology was equivalently precise, but each method recovered...Orr recently earned his Ph.D. in Biology and Biotechnology from Worcester Polytechnic Institute where he...Cas9 and RNAi screens for essential genes. Nat Biotechnol 34:634–636 . https://doi.org/10.1038/nbt.3567...

...reverse transcriptase Article contributed by Will Arnold  Listen to the MarathonRT podcast segment Researchers...potential to provide improvements to existing technologies based on polymerase processivity. Please find...focuses on proteomics: developing and applying new technologies to quantify proteins in tissues, cells, and ...separate plasmid. When cells are treated with biotin–phenol and H2O2, APEX2 labels electron-rich amino acid...-APEX2 fusion.  This Sontheimer lab used this technology, which they call C-BERST (dCas9–APEX2 biotinylation...

...’re trying to make MTA turnaround faster with technology transfer offices (TTOs). The latest of these ...University  Broad Institute  California Institute of Technology Cold Spring Harbor Laboratory  Dana-Farber Cancer...from Addgene MTA: material transfer agreement Technology Transfer: the exchange of intellectual property...universities and other research institutions TTO: Technology Transfer Office. Some synonymous terms include...Addgene.org Addgene Help Center: Tech Transfer Technology Transfer Information Tech Transfer News: A Reintroduction...

... vectors: Enhancement in vector stability." Biotechnology and bioengineering 94.2 (2006): 322-329. PubMed...applying osmotic pressure and sodium butyrate." Biotechnology and bioengineering 42.5 (1993): 601-610. PubMed... stress." Advances in animal cell biology and technology for bioprocesses (1989): 272-278. 4. Ozturk, ...growth, metabolism, and antibody production." Biotechnology and bioengineering 37.10 (1991): 989-993. PubMed...improves adenovirus production." Journal of biotechnology 157.1 (2012): 228-236. PubMed PMID: 22154564...

...molecular biology labs. Recent and rapid advances in technologies that permit large-scale creation and synthesis...overcome crucial challenges in NGS by developing technologies that can help unlock the potential of modern...develop and apply our plexWell™ Library Preparation Technology for NGS-based sequencing and confirmation of ...sequence large numbers of plasmids. plexWell™ technology overview For any NGS experiment, the DNA sample... and CEO of seqWell, Inc. He is a sequencing technologist and computational biologist with a passion for...

... software product manager at Oxford Nanopore Technologies. If the first thing you hear when someone says.... Now, in my current role at Oxford Nanopore Technologies, I work with our customers in the US to learn...Field-based Product Manager at Oxford Nanopore Technologies for the North America region. She is particularly... DNA sequencing tech, and the intersection of technology, software, and science. She loves to advocate... for the customer and help build software and technology tools with the customer in mind. You can follow...

...To avoid confusion, we usually use different terminology when discussing base editors, like calling it...and Resources Glossary We introduced a lot of terminology in this post! Here’s a quick refresher if you...target-library analysis, and machine learning. Nature Biotechnology, 39(11), 1414–1425. https://doi.org/10.1038/...optimization and ancestral reconstruction. Nature Biotechnology, 36(9), 843–846. https://doi.org/10.1038/nbt...targeted DNA transversions in human cells. Nature Biotechnology, 39(1), 41–46. https://doi.org/10.1038/s41587...enable C-to-A and C-to-G base changes. Nature Biotechnology, 39(1), 35–40. https://doi.org/10.1038/s41587...

...generate a targeted break. These advances in gRNA technology have enhanced targeting efficacy as well as the...just one modification alone (Hendel, et al., Nat Biotechnol; Rozners, JACS). These modifications can be done...switches for gRNAs A major drawback of CRISPR-Cas9 technology is that most expression systems are constantly...system can initiate targeted editing events. Both technologies utilize chemical modifications to the RNA to...CRISPR-Cas genome editing in human primary cells. Nat Biotechnol 33, 985–989 (2015). https://doi.org/10.1038/nbt...

...Harvard University), we developed two new enabling technologies for the field of synthetic biology. The first...of the diagnostic potential of these combined technologies, we built paper-based synthetic gene networks...infrastructure of molecular and electronic companion technologies that has transformed the assay’s capabilities...the first time, we have also demonstrated our technology on active virus, as well as shown that we can...finding the resources necessary to advance the technology through product development, the regulatory process...

...Education Project (pgEd). Advances in genetic technologies and other biomedical innovations promise an ...society are to figure out the best way to shepherd technologies through thorny ethical issues, ensure that everyone... fruits of scientific research, and prevent technological advances from exacerbating existing inequalities...about genetics, both as individual consumers of technologies and as societal participants in the political...public health, science policy, and science and technology studies (STS). I also worked with a number of...

...around the globe. Our first story focuses on a technology that has changed virtually all of biology…CRISPR...repository. Other researchers, eager to try this new technology, were quick to request CRISPR plasmids and, in...favorite observations from this data is that CRISPR technology is consistently showing rapid development and...requested through the years, even as more CRISPR technologies become widely used and available. If we look...all of this work helped scientists use CRISPR technology? We think so — and so do our requestors! CRISPR...

...Solvents Organic solvents, generally acetone, methanol, or ethanol, work by dehydrating and precipitating cellular...saturated being 37%). Formalin solutions often contain methanol as well, in order to slow the polymerization of...hidden within the folded protein (green region). Methanol dehydrates the protein, which can disrupt protein...want to fix and permeabilize in one step, then methanol treatment could be an effective strategy. (This...

... invention in the late 1990s, Gateway cloning technology has become very popular as a rapid and highly...and its advantages. An introduction to Gateway technology The Gateway cloning method, developed by Invitrogen...clone in their desired DNA sequences. Gateway technology relies on the two reactions described below: ... more information. How to clone using Gateway technology To better understand the process, we’ll walk ...References 1. Chee JY, Chin CF (2015) Gateway Cloning Technology: Advantages and Drawbacks. Clon Transgen 4:138...

...generating interesting drug-like compounds especially cardenolides which have been used to treat heart disease....disease. Caterpillars that eat milkweed generate cardenolides that tastes yucky (and is poisonous) for bird ...with RNAi to make a polygamous species monogamous. Anolis sagrei (Doug Menke) No methods were available to... to create targeted gene mutations in anoles lizards or in any reptiles at all. But new success is coming... used study the genetic basis of evolution in immunology, neurobiology, and more. Prochlorococcus (Penny...

...Mar 3, 2016 and last updated Apr 13, 2021 by Will Arnold. Although CRISPR systems were first discovered ...MAGE (Multiplex Automated Genome Engineering) technology (Ronda et al., 2016). pMA7CR_2.0 expresses lambda...Scientists have begun to realize the potential CRISPR technology can have on the human microbiome. You can consider...produce sequence-specific antimicrobials. Nat Biotechnol 32:1146–1150 . https://doi.org/10.1038/nbt.3043...efficiently delivered RNA-guided nucleases. Nat Biotechnol 32:1141–1145 . https://doi.org/10.1038/nbt.3011...bacterial genomes using CRISPR-Cas systems. Nat Biotechnol 31:233–239 . https://doi.org/10.1038/nbt.2508...

...antibodies: the power of in vitro display technologies. Nat Biotechnol 29:245–254 . https://doi.org/10.1038...of Milstein and Köhler who developed hybridoma technology. But what about single chain fragment variables...Clinical Application. Clinical and Developmental Immunology 2012:1–15 . https://doi.org/10.1155/2012/980250...(2016) The birth of monoclonal antibodies. Nat Immunol 17:S13–S13 . https://doi.org/10.1038/ni.3608  Monnier...

... editing technologies have been shown to be excellent alternative genome editing technologies especially... single-walled carbon nanotube carriers. Nat Nanotechnol 14:447–455 . https://doi.org/10.1038/s41565-019...confers heritable resistance to powdery mildew. Nat Biotechnol 32:947–951 . https://doi.org/10.1038/nbt.2969...Applications of CRISPR–Cas in agriculture and plant biotechnology. Nat Rev Mol Cell Biol 21:661–677 . https://...