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TypeBlog PostPublishedJuly 9, 2015, 2:30 p.m....: if there is no place to upload a cover letter, make your cover letter the first page of your resume ... don't ask, you will never get the help that can make a difference. Having your resume forwarded personally...
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Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog PostPublishedMay 12, 2015, 1:32 p.m....sought to make complex genome modifications in Drosophila using multiple recombinases. To make multiple... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog PostPublishedSept. 15, 2014, 6:05 p.m....there, but the panelists were interesting enough to make up for the somewhat naïve questions. During the ...coincidentally speak the same Polish sub-dialect making their Skype communications extra fun) who shared... -
London Calling - Addgene’s New Europe Office in London
TypeBlog PostPublishedJune 10, 2014, 3:20 p.m....interacting with people and talking about science makes her the perfect Addgenie and she can’t wait to see...even visit your lab! We look forward to helping you make some serious discoveries. 10 Years of Plasmid... -
CASTing Off for New Shores in Human Genome Editing
TypeBlog PostPublishedJune 10, 2025, 1:15 p.m.....1038/s41586-019-1323-z Peters, J. E., Makarova, K. S., Shmakov, S., & Koonin, E. V. (2017). Recruitment... -
Antibodies 101: Affinity Tags
TypeBlog PostPublishedSept. 19, 2023, 1 p.m.... a little thoughtful planning, affinity tags can make protein purification a cinch. Types of affinity ...that is unlikely to perturb protein function. This makes it amenable to being left on your protein of interest... -
The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place
TypeBlog PostPublishedJan. 21, 2020, 2:30 p.m....in large supplementary data files, BioGRID ORCS makes it possible for researchers to query all public ...conditions. This increased ease of access not only makes CRISPR screen results available to the entire biomedical... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog PostPublishedJuly 16, 2019, 12:59 p.m.... with short generation times and high fecundity, make zebrafish ideal for high throughput studies of optogenetics...depends on these charged particles and whether they make the inside of the neuron more positive or more negative... -
Five Questions to Ask If You're Deciding Between a Master's or PhD Program
TypeBlog PostPublishedFeb. 16, 2021, 2:15 p.m....a means to gain employment, so it’s important to make sure a masters helps you get a job you want. But...bench. So if you enjoy wielding a pipette, it might make sense to earn a master’s instead of a PhD. Deciding... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog PostPublishedSept. 15, 2016, 2:30 p.m....in healthy patients. However, the R206H mutation makes ACVR1 responsive to members of the ubiquitous activin...awareness of FOP. Please consider running with us or making a donation via the link below. Please continue ... -
Management for Scientists: Managing vs. Leading
TypeBlog PostPublishedMarch 31, 2015, 4:08 p.m....in doer mode even late in our careers. Managers make sure the work gets done Managers do this by removing...communication. A manager must both hire and fire to make sure the team is the best it can be. Managers must... -
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio
TypeBlog PostPublishedJan. 15, 2015, 1:50 p.m.... my way to the meeting, and its history of code making and code breaking is wonderfully analogous to genetic...thanks to all the Addgenies who worked so hard to make this poster, offered suggestions and tips, and helped... -
10 Ways to Share Your Science!
TypeBlog PostPublishedMay 13, 2014, 8:34 p.m.... to share your science 1) If you don’t already make it a habit to talk – really talk – about the research...science, they have good advice for anyone looking to make their science matter. If you aren’t ready for a ... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog PostPublishedJan. 30, 2014, 3:29 p.m....scientists rarely stop to think about how much easier it makes our lives. Plasmid transformation into E. coli is... strains have natural antibiotic resistances, so make sure your plasmid and E. coli strain are compatible... -
Choosing a Good Mentor for Scientists
TypeBlog PostPublishedJan. 16, 2014, 2:56 p.m....serve as a good mentor. To read more about what makes a good mentor, see the previous post in this series...posts in our Science Mentors series: Part 1: "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring... -
Science Careers: Unruly Interests Feed Many Paths
TypeBlog PostPublishedSept. 26, 2017, 12:12 p.m....stop for refreshment along the way? What ecosystem makes your heart sing with delight? From developmental...other scientists, or science journalists, or policy makers. The term editor covers a wide variety of careers... -
Safe Port in a Storm...How Addgene is Weathering the Pandemic
TypeBlog PostPublishedSept. 17, 2020, 1:15 p.m....was awed by the teamwork and initiative it took to make all this happen with 80% of the team working remotely...Protection Program loan and received enough funds to make most of our payroll for the spring and early summer... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m....summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...has developed a new CRISPR screening method that makes use of guide RNAs with internal barcodes (iBARs)... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog PostPublishedApril 25, 2019, 3:01 p.m....Biotechnology that now can be deposited at Addgene. What makes these fluorescent proteins unique and what can they...excitation at 493 nm and max emission at 509 nm), making it useful in multicolor labeling particularly with... -
Summer SciComm Series: Master of SciComm
TypeBlog PostPublishedJuly 21, 2022, 1:15 p.m....whirlwind of skill acquisition. Classes covered filmmaking, podcasting, philosophy of science, creative ...misinformation in the wake of a global pandemic can make for a sore reminder of how far we have yet to go...