...pTnsABC, pQCascade and pCutamp.  pDonor carries a customizable cargo gene in the form of RE-cargo-LE (RE, right...and the crRNA. The spacers of the crRNA can be customized as well, including concatenations into crRNA ...

...64 possible codons but only 20 amino acids and 3 stop codons to encode leaving 41 codons unaccounted for...preferences among organisms aren’t completely understood, but some possible reasons include: Metabolic...

...of such targets include transcription factors, histones, and DNA repair enzymes. Note that when using ...stepwise decrease in fluorescence intensity in a (histogram) flow plot (Figure 4b). Through this method, differences...

...respectively. H, A, and W describe the shape of a histogram, which graphically represents the time and intensity...graph of photocurrent vs. time. The resulting histograms is described through maximum current (H), the...

...which one will work as illustrated in figure 3 (Mastop et al., 2018). Unbiased, random insertions can ...Nature methods (2019): 1. PubMed PMID: 30886412. Mastop, Marieke, et al. "A FRET-based biosensor for measuring...

...edition. Cambridge university press, 1988. AAS: Historical Trends in Federal R&D NJ.com: Rush Holt: Lawmakers...affected by Trump’s immigration ban Studies in History and Philosophy of Science Part: Democratic values...

... al is a PCR-based approach for generating a customizable comprehensive mutagenesis library that’s ready...sites in the same orientation as the gene or use custom gene synthesis or site directed mutagenesis to ...

...cloned by two separate labs (Bird et al., 1988; Huston et al., 1988). scFvs are the smallest unit of an.... https://doi.org/10.1371/journal.pone.0189964  Huston JS, Levinson D, Mudgett-Hunter M, Tai MS, Novotny...

...However, ART is not an HIV-1 cure. In patients who stop ART, viral levels will soon skyrocket back to pre-treatment...HIV-infected reservoir, researchers have previously used histone deacetylase (HDAC) inhibitors to increase transcription...

...ICC) when the sample is cultured cells or immunohistochemistry (IHC) with tissues), where an antibody binds....3001161 McLaughlin, K. (2019). Multiplexing Immunohistochemistry. Materials and Methods, 9. https://doi.org...

...pUC multimerization was reduced by growing seed stocks at 30°C compared to 42°C. Screen multiple colonies...platform incorporating low metabolic burden seed-stock and fed-batch fermentation processes. Biotechnol...

...technologies, one might incorrectly relegate RNAi to history. The straightforwardness of RNAi is unmatched—unlike...cannot be used to investigate essential genes. Historically, gene silencing methods were thought to be afflicted...

...recombinase-dependent vector designs used a lox-STOP-lox (LSL) or frt-STOP-frt (FSF) sequence prior to an ATG start...

...Fire AZ (2014) Efficient Marker-Free Recovery of Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis... GM, Calarco JA, Colaiácovo MP (2013) Heritable Custom Genomic Modifications inCaenorhabditis elegansvia...

...conditions (37 °C, LB, antibiotics, etc.) and stored as glycerol stocks We will need information on how to verify...

...generated the first optically active saCas9 protein. Stop light! Photoactive anti-CRISPRs halt Cas9 activity... which would add the PCK upon reaching the amber stop codon, TAG. (To learn more about site-specific incorporation...

...transport in neurons. (b) Genetic cargo can be customized to control transgene expression. Gene regulatory...Chuapoco, M. R., Flytzanis, N. C., Goeden, N., Christopher Octeau, J., Roxas, K. M., Chan, K. Y., Scherrer...

...ELISAs, western blots, immunoprecipitation, immunohistochemistry, immunocytochemistry, and flow cytometry...

...grade DNA!     This is just the first of many milestones Addgene is poised to reach in the near future...

... of Fame  Thursday: 8 Great Depositor Research Stories   Friday: Specialized Depositor Shout-Out  We'll...