...been excitedly testing NgAgo's genome editing applications. So, how does NgAgo stack up thus far? A few..., those who have been lucky with NgAgo should explicitly share tips and protocol details with the scientific... S. Lander, and Feng Zhang. "Development and applications of CRISPR-Cas9 for genome engineering." Cell...

...problem for fusion proteins. One of the major applications of FPs is to visualize the localization, dynamics...even if they were described as monomeric in a publication. The gold standard in assays used to determine...An experimental study of GFP-based FRET, with application to intrinsically unstructured proteins. Protein...

...of LSFM microscopes (3,4). After this initial application, Stelzer’s group described the single-plane or... used a solution of benzyl alcohol and methyl salicylate which was later modified by others to produce...Spalteholz. (1914) Uber das Durchsichtigmachen von menschlichen und tierischen Praparaten. Leipzig: S. Hierzel...

...common antibiotic resistance genes and which applications are best (or worst) for each antibiotic. The...is expensive (relative to amp), and for some applications, the benefit it offers is marginal. Fortunately...antibiotic resistance ultimately comes down to your applications and needs. The more niche and specific your ...

...your experiment. Shorter amplicons tend to be better. Shorter amplicons make for shorter PCR programs...Addgene Blog Learn to do Restriction Cloning Try SLIC cloning Learn about Gibson Assembly Additional ...

...manuscript to get reviewed and edited prior to publication, and this will delay the dissemination of your... makes it easy to annotate protocols, make them public for others to use, and add your own comments. Currently...Freedom: The Expansion of Data Sharing in Research Publications by Jim Woodgett        ...

...our loved ones, and become a positive force for public health. Visit Addgene's Microbiology Pages What...Microbes (bacteria included) are very skilled at the delicate art of survival. Existing virtually ubiquitously...about health equity, science communication, and public health.     References 1. O'Neill, J. "Tackling...

...detrimental as too little, resulting in MECP2 duplication syndrome or Rett Syndrome, respectively. One ...editing, MECP2 reactivation, RNA editing, RNA trans-splicing, and protein replacement to reverse the impacts....0.co;2-o Baker SA, Chen L, Wilkins AD, Yu P, Lichtarge O, Zoghbi HY (2013) An AT-Hook Domain in MeCP2...

...affected by many experimental factors. To list some complicating factors for quantifying AAV: qPCR efficiency...8610.4. Hindson CM, Chevillet JR, Briggs HA, Gallichotte EN, Ruf IK, Hindson BJ, Vessella RL, Tewari M... abundant targets: from variable nonsense to publication quality data." Scientific Reports 7 (2017). PubMed...

...experimental design and its potential for clinical applications. Specifically, efficacy rates of Cas7-11 were...place, while increasing the number of potential applications. But they weren’t done engineering quite yet... at the bench and potentially in therapeutic applications. The potential of CRISPR has yet to fully taken...

...Protein A and Protein G are not suitable for all applications, such as protein isolation from serum. In this...capture antibody interaction and/or the downstream application. Typically, if the target protein will be detected...and an isotype control antibody. Alternative applications Oftentimes, an IP-appropriate antibody simply...

...pooled library DNA, allow the bacteria to grow and replicate, then purify the DNA.     Figure 2: A pooled...protocol. I also recommend searching PubMed for any publications associated with the library and reading their...no more than one viral particle, giving a low multiplicity of infection (MOI). MOI is controlled by viral...

...genomic DNA in many cell types and often results in biallelic modifications. Most commonly, DSBs are repaired.... Furthermore, CRISPR/Cas9 editing is often bi-allelic, meaning that even if one allele is accurately ...heterozygous mutations by multiplying the single allelic mutation incorporation probabilities our data revealed...

...study its function is one of the most popular applications of fluorescent proteins. These fusion proteins...linker sequence short In the early days of GFP applications, many were concerned with steric hindrance by...between secondary structure (beta-sheets or alpha helices). An additional requirement is that the site of...

...strand. While this pathway may not be immediately applicable in DSB-induced genome engineering, it has biological...that can be harnessed by researchers for many applications. Your favorite Cas enzyme, a guide RNA to your...Mouse Geneticist's practical guide to CRISPR applications. Singh P, et al. Genetics 114.169771; (2014)...

...comparison to a control sample. Southern blotting applications range from confirming the results of cloning...sequence thousands of reads at the same time. Other applications of sequencing, such as confirming the results...replaced these, these techniques still have useful applications and have informed many current techniques. For...

...stimulation. GPCR signaling GPCR signaling is a complicated process involving many downstream signaling mechanisms...break your experiments!  First, closely read the publication, including the supplemental data. You’re most... both of which have extensive collections of publications and information on hardware, optogenetics tools...

...source of piggyBac™ transposase (available from a licensed distributor) allow you to quickly transfer a DNA...piggyBac™ technology can be used for numerous applications, including transgenesis, gene-trap screens, ...technology that may be used for a variety of research applications, including making transgenic cell lines and ...

...vivo animal model should consider the possible elicited immune response from viral infection. Animal safety...rate limiting step and can immediately undergo replication and transcription. However, with scAAV the effective...only can we compare these methods in specific applications, we may also be able to use these comparisons...

...genetic code of living organisms and its societal implications even before the discovery of genes themselves... that revolves around synthetic humans called replicants which are genetically engineered by a powerful...us to always consider the ethics and broader implications of our research.  Want the Genome Editing Trading...