...expect given the excitement surrounding anything to do with CRISPR, our most popular post in July (with ...

...wild-type and engineered variants of Cas nucleases. In doing so, they also created a modular toolkit for Cas-...

...all the excitement and speculation, we thought we’d do a little digging and assemble a rough guide to the...

...to make all 12 possible base-to-base changes. How do base editors work? Base editing requires three elements...to use the deaminated DNA strand as a template. To do so, the lab used a Cas nickase, instead of dCas9....editors often produce a mixed population of edits, ABEs do not display significant A to non-G conversion at ...

...editor. Cas13 enzymes are advantageous because they do not require a PAM, and RNA targeting is potentially...flanking sequence (PFS) adjacent to the target, but many do not, showing the flexibility of this system.  As ...

... using site-directed mutagenesis of Spinach. In doing so, they identified the nucleotides that contribute...Just as vegetables come in a variety of colors, so do aptamers! Corn was recently described in 2017 (Song...

...being published on our own topic. Only on occasion do we then have time to read about research from other...otherwise never learn from the valuable research we are doing. Browse the site, learn from others, share ideas...

...Expression in Mammals AAV Titers: Where do they come from and what do they mean? A Match Made in Heaven: CRISPR...

...array of base editing reporters that can help you do just that. Monitoring APOBEC and Cas9-mediated editing...tested their hypothesis with GFP fluorescence. To do this they generated a silent GFP construct, that ...

...tool that is easy to use with other plasmids.  How do you know if your plasmids are incompatible? Scientists...will be based on sequence data. However, if these do not provide clarity, a wet lab transformation experiment...

...guest blogger Derek Simon. The actual experiments you do will be determined by the topic you’re interested...Second, this may sound rudimentary, but before you do any type of behavior analysis, simply take a few ...

...Education & Tools menu Further down What happens if you do scroll down? You’ll find Blugene, as well as information...

...yourself, you’re probably wondering: What does it do and how does it work? LentiCRISPR v2 purpose As its...

...students next year! Does your lab or organization do work to help improve K-12 education? Let us know ...

...less likely to alter a protein’s properties. Why do we need new epitope tags? Short epitope tags like...

...alone. (Seriously, is there anything CRISPRs can't do?) Structural details All this, and scientists are...

...of people willing to make that idea a reality! How do you share your science with the community? Addgene...

...it. 6. Edit your DNA sequence Finally, when you do see a miscalled peak, don’t be shy. Feel free to ...

...available through Addgene, and they hope others will do the same. “This isn’t the final version,” she said...

...tools in C. elegans and in Plasmodium. For those who do want to apply LITEs for the transformation of neurons...exposure. Third, the response can be graded with light dose and is fully reversible after light retraction. ...