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Showing: 321 - 340 of 695 results
  1. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...a given phage will recognize on a host cell. To make matters even more interesting, much of what a phage...display little to no homology to anything else, making it difficult to quickly identify possible gene ...the T7 polymerase and a few proteases knocked out making them particularly useful for protein production...
  2. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 3:42 p.m.
    ...2008). It can also cross the blood-brain barrier, making it useful for applications targeting the CNS (Issa...expression (Tervo et al., 2016). This functionality makes it useful for mapping neural connections. Although...depends on your experimental system. As we cannot make universal serotype recommendations, the following...
  3. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...they are relatively small and easy to work with, making them the go-to choice for studying mammalian biology.... For editing the genome, the CRISPR/Cas system makes use of 3 components, a guide RNA (gRNA) of about...content in the mouse genome and the Cas9 enzyme will make a cut at the target site. This is when it gets exciting...
  4. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...our lab so we opted for AAV. While these benefits make AAVs useful for a wide variety of experiments, care...can still reduce AAV titer. For long term storage, make > 25 μl aliquots in 0.5 ml tubes, snap freeze in...humans and additional improvements are being made to make them even more useful for therapeutic purposes. ...
  5. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...characterized by large DNA recognition sites, which makes them highly specific.  Jurassic Park by Michael ...   Star Trek: Into Darkness (2013) which is the remake of the space-melodrama Star Trek II: The Wrath ...has always served as a vision of the future and to make us reframe our perspective of the current. Although...
  6. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...with DIO/FLEx vectors and off-target expression making it easier to determine the spread pattern around...your cells of interest. You should now be able to make smaller adjustments to a set of injections into ...K, Sanders E, Tasissa M, Kostman M, Tillgren M, Makana Hanley L, Mueller I, Mitsopoulos A, Fan M (2020...
  7. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...ve been planning, it is a good idea to pause and make sure that your antibody has been appropriately validated...guarantee recognition of the target antigen. You want to make sure that your antibody binds to what you expect... epitope information for some antibodies, which makes it hard to ensure that you have unique antibodies...
  8. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...characteristics to consider when selecting a dye or ligand to make your selection easier, as well as highlight some... imaging tools in biological research. This does make finding the right dye for your experiment more complicated..., M., Cincotti, G., Rampioni, G., Visca, P., & Kolmakov, K. (2023). Expanding the microbiologist toolbox...
  9. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...enjoying the party. There are so many teams that make Addgene functional, handling everything from facilities...this diverse group of talented individuals that makes Addgene — well, Addgene. We're so grateful for each...
  10. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ...that we’ve learned in the lab that will hopefully make your life a little easier. These can range from ...Putting on lab gloves can sometimes be annoying. To make it easier, try this quick trick. Just flip, and ...
  11. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    Published
    Nov. 14, 2013, 4:10 p.m.
    ...get to talk to scientists from around the world making breakthrough discoveries!” I knew right then I ...way that a connected and collaborative community makes not just for a better day at the office but for ...
  12. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ...samples from my assay at like 23:00, so I decided to make a giant snow angel for biologists in our building...performing routine lab maintenance tasks. Hot tip: make sure your PI/facilities are okay with the decorations...
  13. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    Published
    March 2, 2021, 2:15 p.m.
    ...Expressing these chromoproteins in E. coli would make it easy to distinguish colonies from one another...always get a few isolated colonies. I should probably make it a bit more challenging, potentially by using ...
  14. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    Published
    Nov. 16, 2020, 2:15 p.m.
    ...wanted to take a week to celebrate the scientists who make a big impact in open science. Depositors consistently...this year, Addgene depositors quickly stepped up to make COVID-19 reagents available to researchers across...
  15. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    Published
    Sept. 1, 2016, 2:30 p.m.
    ...sometimes be elusive or have a certain mystique that makes them difficult to approach for the youngster just...people to do science? 16:08 - 22:04 : How have the make-up and scope of your graduate projects changed throughout...
  16. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    Published
    March 26, 2015, 3:24 p.m.
    ...providing quality control and customer support, making plasmids easy for you to find and request in our... plasmids as of March 26, 2015. How does Addgene make it happen? Our awesome team of Addgenies! Our team...
  17. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...only viral protein present on the virion surface – making it critical for viral attachment and fusion. Once...  The EBOV glycoprotein’s role in infection also makes it a critical component of vaccines and the target...
  18. Addgene's Website Update

    Type
    Blog Post
    Published
    April 8, 2024, 2:15 p.m.
    ...changes to our website! Part of sharing science and making access to materials easier is improving the tools...may be less obvious — but every change is meant to make your experience on our website better so you can...
Showing: 321 - 340 of 695 results