...minor extension can usually ensure that the 3’-base(s) do not form secondary structures. The introduction...

... tactic, and puts you ahead of most start-up P.I.’s. It doesn’t have to be complicated, or difficult to...

...PMID: 25895059 Barnea, et al. Proc Natl Acad Sci U S A. 2008. PubMed PMID: 18165312 CIDAR MoClo parts ...or clusters. Veggiani, et al. Proc Natl Acad Sci U S A. 2016. PubMed PMID: 26787909 EasyClone 2.0 Yeast...Hanson SM, Rodríguez-Laureano L, Albanese SK, Gradia S, Jeans C, Seeliger MA, Levinson NM, Chodera JD. bioRxiv...

...antibiotics! References Espah Borujeni, Amin, Anirudh S. Channarasappa, and Howard M. Salis. "Translation ...

...24700457. PubMed Central PMCID: PMC4077948.  Yan, Kelley S., et al. "The intestinal stem cell markers Bmi1 and...

...Addgene. Wang Z, Pan Q, Gendron P, Zhu W, Guo F, Cen S, Wainberg MA, Liang C. CRISPR/Cas9-Derived Mutations...

...: 19363495. 2. Wang JW, Wang A, Li K, Wang B, Jin S, Reiser M, Lockey RF. CRISPR/Cas9 nuclease cleavage...

... approachable relationship for the mentee, where (s)he feels truly understood and supported, and can more...

...Common gene mutations found in E.coli strains Gene(s) Description Functional Consequence dam DNA adenine...

...1:24 - 29:31 - Reproducibility Overview - Jeffrey S. Flier, Researcher at Harvard Medical School, former...

...27786307. PubMed Central PMCID: PMC5476805. 8. Inagaki, S., et al. (2017) Genetically encoded bioluminescent...

... Kang SH, Bae YJ, Hong JT, Chung YB, Lee CK, Song S (2006) PCR-based detection of mycoplasma species. ...

...do in the future. Questions to ask: Will someone(s) train you technically? Will the PI/supervisor look...

...that the output text files can be hard to read. It´s suggested that you open the above text files, copy...

...applied research since their first use in the early 90’s to genetically modify primary cells. Amongst the different...

...-100 nucleotides long with the desired alteration(s) located in the center of the sequence. Since lambda...

...Being a scientist in my late 20’s, new graduates often ask me for advice on careers available to new ...

...avenues in healthcare.   References 1. Vinay, Dass S., et al. "Immune evasion in cancer: Mechanistic basis...

....17912/DDVH-BG64 Tzur YB, Friedland AE, Nadarajan S, Church GM, Calarco JA, Colaiácovo MP (2013) Heritable...

...10.1039/c8en01381k Sridharan, R., Zuber, J., Connelly, S. M., Mathew, E., & Dumont, M. E. (2014). Fluorescent...