Addgene

We narrowed to 321 results for: NUC

Showing: 301 - 320 of 321 results

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Fluorescent Proteins 101: When GFP lets you down

Type

Blog Post

Published

Aug. 23, 2018, 12:05 p.m.

...loop-structure (Querido and Chartrand, 2008). Finally, a nuclease-deficient Cas9 can bind loci in the genome that...
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

Type

Blog Post

Published

Dec. 2, 2019, 2 p.m.

...to specific subcellular locations, such as the nuclear envelope, allowing scientists to probe interactions...
Multiple Plasmids at a Low Price: Kits from Addgene

Type

Blog Post

Published

Sept. 14, 2016, 2:30 p.m.

...Choudhury, Rajarshi, et al. "Engineering RNA endonucleases with customized sequence specificities." Nature...
Using Phosphoserine to Study Protein Phosphorylation

Type

Blog Post

Published

June 23, 2016, 2:30 p.m.

...PhosphoSitePlus, 2014: mutations, PTMs and recalibrations." Nucleic acids research 43.D1 (2015): D512-D520. PubMed ...
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Type

Blog Post

Published

May 10, 2016, 2:30 p.m.

...this approach doesn’t require a functional Cas9 nuclease, so there’s no potential for aberrant DNA cleavage...
New Optogenetic Tools for Cytoskeleton and Membrane Control

Type

Blog Post

Published

Sept. 28, 2023, 1:15 p.m.

...by Rho family signaling pathways. Rho guanine-nucleotide exchange factors (GEFs) activate their partner...
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

Type

Blog Post

Published

April 24, 2015, 2:39 p.m.

...inversion of the construct. Sanes and Lichtman used pronuclear injection to insert these Brainbow constructs...
Technique: Probe Phage Genomes for Host Binding Proteins

Type

Blog Post

Published

Sept. 29, 2016, 2:30 p.m.

... in order to protect their DNA from host cell nucleases (4). To further complicate matters, even when ...
Antibodies 101: The Basics of Western Blotting

Type

Blog Post

Published

March 8, 2021, 2:41 p.m.

...compartments such as the mitochondria, cytoplasm and nucleus. We hope that this blog has provided you with ...
Mycoplasma Contamination: Where Does It Come From and How to Prevent It

Type

Blog Post

Published

Oct. 8, 2020, 1:15 p.m.

...result interpretation. Also, the signals from the nuclear area make the detection of mycoplasmas difficult...
Quick Guide to All Things Lentivirus

Type

Blog Post

Published

March 21, 2017, 2:30 p.m.

...LTRs) has been removed, Rev (which facilitates nuclear export) is expressed from a separate plasmid, and...
With an Eye Towards the Future, We Look Back at the March for Science

Type

Blog Post

Published

Oct. 24, 2017, 1:54 p.m.

... Nicholas Thompson is a PhD Candidate studying Nuclear Science and Engineering at Rensselaer Polytechnic...
Custom CRISPR Screens & the Green Listed Software

Type

Blog Post

Published

July 11, 2017, 2:30 p.m.

...and libraries using strategies related to other nucleases as well as inhibitory and activating approaches...
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

Type

Blog Post

Published

April 25, 2017, 2:30 p.m.

...Mizuno, and Atsushi Miyawaki. "Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting...
Special Delivery: Fluorophore Targeting for FRET Studies

Type

Blog Post

Published

July 19, 2016, 2:30 p.m.

...2007). All that is required is that the proteins/nucleic acids/lipids of interest are fluorophore labeled...
A Primer on Optogenetics: Introduction and Opsin Delivery

Type

Blog Post

Published

Sept. 10, 2015, 2:30 p.m.

...VTA) but wish to stimulate VTA terminals in the Nucleus Accumbens (NAc; a primary target of VTA projections...
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation

Type

Blog Post

Published

Nov. 6, 2018, 1:12 p.m.

...References 1. Hochedlinger, K. and R. Jaenisch, Nuclear reprogramming and pluripotency. Nature, 2006. 441...
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

Type

Blog Post

Published

Nov. 3, 2015, 3:30 p.m.

..."DNA targeting specificity of RNA-guided Cas9 nucleases." Nature biotechnology 31.9 (2013): 827-832. PubMed...
Technical Design of a Western Blot

Type

Blog Post

Published

Aug. 22, 2024, 1:15 p.m.

...membrane-bound subcompartments, like the mitochondria or nucleus, will likely require a harsher lysis buffer than...
Using AAV for Neuronal Tracing

Type

Blog Post

Published

Aug. 9, 2018, 1:04 p.m.

...Retrograde AAV: Making the journey from axon to nucleus Rabies and neuronal tracing Important considerations...

Showing: 301 - 320 of 321 results

Results per page:

Fluorescent Proteins 101: When GFP lets you down

Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

Multiple Plasmids at a Low Price: Kits from Addgene

Using Phosphoserine to Study Protein Phosphorylation

Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

New Optogenetic Tools for Cytoskeleton and Membrane Control

Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

Technique: Probe Phage Genomes for Host Binding Proteins

Antibodies 101: The Basics of Western Blotting

Mycoplasma Contamination: Where Does It Come From and How to Prevent It

Quick Guide to All Things Lentivirus

With an Eye Towards the Future, We Look Back at the March for Science

Custom CRISPR Screens & the Green Listed Software

Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

Special Delivery: Fluorophore Targeting for FRET Studies

A Primer on Optogenetics: Introduction and Opsin Delivery

Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation

The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

Technical Design of a Western Blot

Using AAV for Neuronal Tracing

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