...sample. Therefore, when a large number of optical slices are acquired all fluorophores throughout the sample...manufacturers have similar information References 1. Lichtman JW, Conchello JA. Fluorescence microscopy. Nature...2005). PubMed PMID: 16299476. 2. Conchello JA, Lichtman JW. Optical sectioning microscopy. Nature methods...2005). PubMed PMID: 16299478. 9. Richardson DS, Lichtman JW. Clarifying Tissue Clearing. Cell 162, 246-...

...up. Applications of photoactivatable fluorescent proteins Some of the most important applications for ...level of background fluorescence. For any PA-FP application, the brighter the fluorescence the better. More...photostable green and red states. Beyond these imaging applications PA-FPs have been developed for use in yeast,...

... required for a growing organism to make the complicated transition from a clump of cells to a fully differentiated...track where GFP labelled B cells were in mounted slices of these organs. These GFP labelled cells could...been created to fate map Langerhans cells and follicular dendritic cells within the skin and lymph node...of antiviral CD8+ T cells in the peripheral interfollicular region of lymph nodes." Nature immunology 9.2...

...cellular impediments. FRET has been used in many applications, including drug discovery by big pharma (i.e...innovation, valuable commodities in any NIH grant application! Using FRET, you may gain a new structural understanding...experiments. Donor Acceptor Tag R0 (Å)a Range (Å)b Applications CFP   Lumio Green Tc: CCPGCC 50 40-72 Intact...discovery–a review of theoretical aspects and recent applications." Current Chemical Genomics and Translational...

...specific subjects in the interview. Happy Listening! Click here to listen! Podcast breakdown: (Time : Topic... PMCID: PMC4096992. Find plasmids from this publication at Addgene. 2. Tang, Jonathan C.Y., et al. “...PubMed PMID: 26258682. Find plasmids from this publication at Addgene. Resources on the Addgene Blog Read...

...expected when we originally sent out the call for applications. Cervical cancer screening in Ghana Gladys is...Papillomavirus (HPV). As Gladys wrote in her application, “Cervical Cancer is the leading cause of cancer...Seeding Labs Plasmid Grant to a third round of applicants at the start of 2017 and would like to thank ...

...Dr. Bengyella, this class of tumors is “a major public health problem, and a leading cause of hospitalization...concluded, Addgene and Seeding Labs will be opening applications for a second round of plasmid awards on June...excited to discover what new and creative ideas applicants will propose for plasmids from the Addgene community...

... upstream of the sequence of interest. 5. Use a silica spin column for purification of the samples you...Acetate/isopropanol precipitation method or using a silica spin column available from several vendors. The...image below), so I would strongly recommend using a silica spin column. They can be pricey, but well worth...

... about intellectual property rights, pending publications, and commercial use often come up. An MTA can...trials Acknowledgement in recipient’s future publications relating to the material Limited liability...created by the Recipient Right to file patent applications on modifications or inventions Right to publish...

...effectors (TALEs) as anchors for genome engineering applications was still very new. Now, Konermann and her colleagues...In an accompanying Nature News & Views Andreas Möglich and Peter Hegemann described several advantages...disease states. While LITE plasmids have clear application to anyone with an interest in neuroscience, their...

...to encourage constructive input from your team: Solicit feedback regularly and often A one-time event ...ask direct questions for specific feedback in non-public settings. Ask over coffee.  Have regular 1:1 meetings...information to make change whenever possible. Be public about changes influenced by feedback. If you don't...

... at a local bar. It doesn't have to be crazy complicated! Listen to the full podcast for more great science...the goal of communicating science to the general public and training the next generation of science communicators...source of scientific information for the general public as well as a platform for scientists to be viewed...

...with the gRNA), application, cas9 species, PubMed ID, and depositor. The CRISPR applications we currently...and final experimental outcome in the original publication. As you develop and confirm new gRNAs, please...

...obstacles, and take steps to resolve interpersonal conflicts in the lab. Yearly planning meetings may not look...Paperwork. Vincent BJ, Scholes C, Staller MV, Wunderlich Z, Estrada J, Park J, Bragdon MD, Lopez Rivera...Science Career Path 7 Simple Strategies to Resolve Conflict With Difficult Supervisors Management for Scientists...

...our site. Simply click on the icon, search for answers, and choose a result. By clicking on the result links...have the option to “Leave us a message.” When you click on the "Leave us a message" button shown to the ...

...Janssen Award dinner which took place at the New York Public library. Being in the presence of scientific heroines...adaptive bacterial immunity and its potential applications in rewriting the genome. Their award acceptance... area will accelerate the launch of clinical applications. During the panel discussion, Dr. Mello took...

...postings looking for PhD applicants since many will also accept applications from master’s candidates ...technical or product support specialist,” “field application scientist,” or “scientific or technical writer...

...should be. He debated taking a position as an application support/sales representative in industry or a...communicator than a science writer these days. I do some public speaking and a bit of video work for webinars (...illustrations for summarizing mechanisms and applications that are relevant to things IDT does. I also...

...+ over other Na+ and other cations. B) Cortical slice of HcKCR1-EYFP and tdTomato expressed layer 2/3 ...blot, but we are actively testing it in other applications! If you try it out in a new assay, let us know...GFP-smFP-myc expressed from Plasmid 98926. See the Applications section on the Anti-c-Myc [9E10] page for more...

...: Find sequences for plasmids assembled from publications and commercially available sources, as well ...making it ready to copy and paste into other applications Primer design tools Many of the above-listed... well as primer design for different cloning applications. Primer3 and Primer3Plus: For general primer...