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  1. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...activation from the 5’ LTR) and Rev (facilitates nuclear export of transcripts). A different viral envelope...

  3. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...with SRM techniques, and adapted for detecting nucleic acids as well as proteins (Klimas, et al. 2023;...

  6. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ... with a tag that localizes your protein to the nucleus? Or you want multiple rounds of purification to...

  7. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...retroviruses. Lentiviruses are able to enter an intact nuclear membrane allowing them to infect both dividing ...

  11. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ... able to label proteins moving to and from the nucleus, ER, mitochondria, stress granules, and more. For...

  12. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...responsive element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for...

  14. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...Physical and genetic studies with restriction endonucleases on the broad host-range plasmid RK2. Molecular...

  15. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...scatter roughly measures granularity, a proxy for nucleic complexity of the cell.  The sample will have been...

  16. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...have accumulated more than 300,000 individual nucleotide sequences. More than half of these sequences ...

  17. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...Retrograde AAV: Making the journey from axon to nucleus AAVs CREATEd for gene delivery to the CNS and PNS...

  20. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...like those that overexpress the toxic protein α-synuclein (the most common protein found in lewy bodies)...
Showing: 281 - 300 of 321 results