...together long enough to get it into cells and let them do the repair work. The key is T4 DNA polymerase, which...and its low cost makes it ideal for researchers doing large amounts of cloning. Assembly is scarless, ...

...Frequently asked questions about γ-retroviral vectors How do engineered γ-retroviruses differ from lentiviruses...promoter is no longer present in these constructs. Why do many γ-retroviral plasmids have similar names? The...

...questions: What is the goal, who is my audience, and what do I want to be different for them when I’m done? What... Excellent! We asked our workshop participants to do the same thing, and even though only about 10% of...

...site specifically incorporated ncAA, ready for you do to any number of experiments with. The catch The...broadly useful for biomedical researchers. One way of doing that is by using Addgene’s Blue Flame award to identify...

... enter the nucleus through nuclear pores and thus do not require breakdown of the nuclear envelope during...division to enter the nucleus as gamma-retroviruses do. Fun fact: Since lentivirus and gamma-retrovirus...

.... That’s also a great way to get to know people doing interesting and relevant work in your field! However...single well in a 96-well (or 384-well) plate! How much do repositories help?   Designing precise calculations...

... time and budget you cannot test them all, so how do you know which ones to try?  Read on and we will ...turn to for advice if you need to troubleshoot. What do you know about the vendor and the vendor’s technical...

...and a quick zap of the proper color light, you can do just that.  Photosensitizing dyes and proteins have...than shorter wavelengths, which makes it possible to do things like in vivo imaging.   Excitation with NIR...

...fallen into studies of who’s there and what are they doing. To address these questions, biologists often use...transcripts gives clues into what the microbes are doing. But aside from sequencing, scientists can also ...

...If you’re using CRISPR to make a genome edit, how do you know if your CRISPR experiment was successful...experiments. But how? There have been several systems to do this but most are labor-intensive, require multiple...

... scifi movie? Or maybe it’s a biosensor?! You can do more than just ‘sense’ biological compounds and reactions...activities.       Viruses and biosensors So how do viruses factor into all of these diverse biosensors...

...experimental conditions. This is time well spent and, if you do it right, can be (part of) figure 1 of your next ...reported properties of fluorescent proteins, how to do a head-to-head comparison of fluorescent proteins...

.... Unlike cytoskeletal polymers, however, R bodies do not exert force by dynamically rearranging into different...bodies can be found at Addgene here. What can R bodies do for you? R bodies are robust linear actuators that...

...each one and make sure it’s working. You can only do that screen with a system you are really confident...depending on which guide RNA we use. What we have to do now is learn more about the rules.” He said that ...

...to tell a story - convince your audience you are doing amazing things."  As Sample6 raised more money, ...seeking funding, ask yourself if you really want to do this.” Biotech startups are only successful if they...

... the bacterial elements previously described. Why do scientists use yeast vectors? Yeast are eukaryotes... Plasmids for use in S. pombe, on the other hand, do not require a well defined ORI. Instead, the size...

...use, of course, depends on what you are trying to do – there’s no “best” tool among them. While this seems...gRNA depends an awful lot on what you are trying to do: gene knockout, a specific base edit, or modulation...importance in design – an optimized sequence will do little if it is in the wrong place, but because the...

...’ll need to align all these images once taken. To do so, you will need to include a marker like the Hoechst...needs, your sample, and targets, you might decide to do simultaneous IF instead (Figure 1B). To label all...important consideration when deciding whether you can do simultaneous or sequential labeling is what conjugated...

...exciting trends in your experimental groups, but how do you determine if they deserve that oh-so coveted ...can be run on these tests. All sample comparisons Do you really need to know every pairwise comparison...mathematician to select the right statistical test, just do your homework and happy testing!   References Wissing...

... for your cell lines and/or tissue types.  Please do note my oh-so-subtle use of reference genes, plural...reference genes - and it is well worth your time to do one (Biassoni, 2014). At the bench As a highly sensitive...you from understanding how the analyses work - you do need to make sure the assumptions in your dataset...end of your analysis process. You’ll still need to do statistical analysis to determine if there are any...