...head-to-head comparison of fluorescent proteins and how to make a well-informed decision on the best fluorescent...properties of fluorescent proteins on websites. To make the best choice, the fluorescent protein properties...than the theoretical brightness. Consequently, it makes sense to identify a couple of promising fluorescent...

... continues to make improvements to our website and inventory management systems to make way in our systems...plasmids in the repository. The Outreach Team has been making sure scientists know about the new initiative and...

...is the fact that Cas9 can be easily programmed to make a DNA double strand break (DSB) in the genome wherever...cleaning enzymes (Aprataxin, Tdp2) that function to make ends better substrates for ligation. Although we...Cas9-generated breaks is useful if the intent is to make a knockout in your gene of interest, as it is prone...

...20,000 genes, it is not possible to test each one and make sure it’s working. You can only do that screen with...system by screening for genes that, when turned on, make melanoma cells resistant to treatment with a BRAF...please ensure that you are equipped and authorized to make and use lentivirus. Also, note that due to the size...

...examples of the toolkit in action, see Cermak T, et al. 2017. Cermak T, et al. Plant Cell. 2017. PubMed ...functions and applications. We hope that these articles make it easier for you to find and use the plasmids you...that is easily separable from that of EGFP. This makes mCyRFP1 a powerful tool for use as a donor in FRET...

...and, most importantly, any connections the neurons make to other distant brain areas. It is desirable for...particles are relatively small (20 nm) in size which makes them capable of diffusing in the extracellular space...particles will infect neurons at different ratios, making these neurons fluoresce in different colors. An... Resources on the Addgene Blog Retrograde AAV: Making the journey from axon to nucleus Rabies and neuronal...

...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...inhibition is spatially and temporally ubiquitous, making them a rather blunt instrument for controlling ...al, Nat Chem Biol 2018. PubMed PMID:29483642. Making protein-protein interaction studies easier with...

...scientists starting their own labs get excited about making their first deposit to Addgene. We are also ...cutting edge practices and benefits and these are making a difference. We serve scientists around the globe...about being able to do good in the world. We are making an impact.  {% video_player "embed_player" overrideable...

...Palladian Partners. “I was lucky to have been able to make that transition really smoothly,” says Susan. After... a very painful process. And certain stories can make you feel like ‘I’m a terrible writer. I have never...science communicators. “The power of a story is to make a human connection with a reader, whether it’s a...

...end, you’ll have a better understanding of what makes polyclonal antibodies unique and what experiments...together, all the B cell clones in the immune system make different isotypes of antibodies that recognize ...other antibody formats. This low barrier to entry makes polyclonal antibodies a common reagent in many labs...

...and share some thoughts from the community that makes us… us. “The Addgene not-for-profit is an amazing...resource. I really appreciate the work you do to make sharing reagents and protocols so easy!” — Nina ...people knew how to access Addgene, and that we were making it possible for scientists to both deposit and ...

...one light chain. When assembled, the four chains make a Y-shaped protein that can be divided into the ...use Fab fragments as primary antibodies, you can make your own antibody fragments as needed.  In contrast...format. Reporter-conjugated anti-IgG Fab fragments make good secondaries for cases where you need to pre-form...

... always excited when depositors find new ways to make this workhorse protein even more useful! From FPs...retain normal neuronal function, this system may make optogenetics in specific cell populations even easier...applications, including optogenetics, and they should make it much easier to conduct functional studies in ...

...plasmid such as pX330. This cloning strategy not only makes it easy to create a single gRNA-expressing plasmid...clone up to 7 gRNAs into one destination vector, making multiplexing easy.  Disadvantages of Golden Gate...TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt...

...spatiotemporal control or an “on/off switch.” This makes them great tools for providing a global overview... of the bait protein will be biotinylated. This makes it’s impossible to distinguish proteins that directly... proteins. This extended incubation period could make it difficult to detect proteins with short half-...

...precipitates that permanently stain the membrane, making that section of the blot unusable for reprobing... if the mild is not effective. You may choose to make your own stripping solution using a recipe like ... Assess the stripping Now it’s time to check to make sure the antibodies from your first round of detection...

...it also helps you to hone your writing skills and make a name for yourself. Pick a topic that is close ...’t you record a podcast or put some effort into making a video? Use your unique talent to communicate ...Since then, I have tried many things: photography, making videos, presenting in pubs and at science festivals...

...proteins had to be re-engineered for each gene, making wide-scale gene activation seem next to impossible...activation compared to other CRISPR activators, making it a popular method for gene activation experiments...design (SunTag). This streamlines its delivery, making it a common choice for CRISPR activation. Newer...

...crucial if we want to make sure that everyone can be informed and empowered to make their own decisions ...posts, lesson plans, or information briefs for policymakers. The rapidly changing landscape of genetics ...

...Requiring an additional plasmid for transfection can make these optimization experiments a bit more challenging...experimental volumes accordingly. While you may need to make some final adjustments, this is generally a good...additional resources. References Kumar M, Keller B, Makalou N, Sutton RE. Systematic determination of the packaging...