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Showing: 261 - 280 of 595 results

  1. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ...contributed by Daniel Bauer and Matthew Canver of Boston Children’s Hospital and Harvard Medical School....pediatric hematologist and principal investigator at Boston Children’s Hospital and Harvard Medical School....

  3. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    Published
    July 20, 2017, 2:30 p.m.
    ...small molecules for use in the clinic and thereby restore motor neuron function in spinal muscular atrophy...mesothelioma sufferers. This disease is less widely understood and often misdiagnosed. Like other forms of mesothelioma...

  4. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ...dictate the space. We wanted to comfortably tell our story, to get scientists excited about what Addgene has...redesigning the homepage, and striving to tell our story in better ways, we realized that Blugene needed ...

  5. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ...require a bachelor’s degree if you have a PhD. 4) Customize cover letters and even resumes The cover letter...project managment) your resume should also be customized to highlight different skills and to get the ...

  6. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...bisporus.) Reporter constructs equivalent to site-STOP-site-myrRFP, with “site” representing the specific...resulted in complete or near-complete excision of the stop sequence. In contrast, Dre recombinase, the close...

  7. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ...lentiviral CRISPR library.  “This enables you to do customized genetic modification on a scale that was really...Using CRISPR/Cas to Edit Disease Out of the Genome History of CRISPR Cas - A tale of survival and evolution...

  8. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    Published
    June 11, 2019, 1:16 p.m.
    ...Biorender workspace. Free Biorender accounts can store 5 workspaces at a time and workspaces are only downloadable...scientists 👨🏽‍🔬👩‍🔬! We built this so you could stop struggling to make figures in PPT 🔬 Also, the base...

  9. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...the fundamentals. Our mission is to curate a one-stop reference guide for plasmids, so that you can spend...and references, and how to maintain your plasmid stocks. The guide also contains multiple protocols and...

  10. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...collapse E. coli re-express arg1 gas vesicles and restore their ultrasound signal. Using acoustic reporter...range of  the ~1011 commensal bacteria per gram of stool for humans. Bourdaeu et al also used arg1 to in ...

  12. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    Published
    May 4, 2023, 1 p.m.
    ...and Resources References Fellmann, C., et al., Cornerstones of CRISPR-Cas in drug development and therapy...CRISPR Guide CRISPR Plasmids and Resources CRISPR History and Development for Genome Engineering    ...

  13. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    Published
    July 7, 2016, 2:30 p.m.
    ...sharing services, as well as #happydepositors who stopped by to say hello. Needless to say we all got very...the conference and talked to some scientists who stopped by the booth about amazing work already represented...

  17. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...interest for which you have several antibodies in stock. Why are there so many options for a single target...samples. If, however, you are performing an immunohistochemistry assay, you may want to rule out monoclonal...

  18. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...Blugene and I represented Addgene at the recent Keystone meeting on Precision Genome Editing with Programmable...publications CRISPOR gRNA design tool Researchers at the Keystone meeting had great things to say about CRISPOR,...

  19. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...after subtracting out non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins...protein folding (tested using real science). Make a stock solution of 2.5 mM biotin (50X) because 5 mM is ...

  20. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...this sequence. The energy from this breakage is stored as a covalent bond between the cleaved 3′ DNA strand...are planning to transform right away OR you can store the reaction at -20C overnight. 4. Transform TOPO...
Showing: 261 - 280 of 595 results