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We narrowed to 622 results for: Dos

Showing: 261 - 280 of 622 results

  1. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...to infect with the virus. Then the viral particles do the rest! Downstream expression or integration resulting...biological outcome you want to achieve? For example, do you want the nucleic acid cargo to be integrated ...

  2. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...ends results in the production of blue colonies that do not need to be picked and sequenced for potential...PCR, restriction digest, or sequencing. Pro tips Do not add 5’ phosphates to your PCR primers; you need...

  3. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...Rooms to divide the audience into small sessions and do a quick break to chat. This can be done randomly ...from academia to industry”. To date, aside from doing science, he has assisted many researchers in gaining...

  4. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ..., but from what was necessary in order for you to do your research. For example, did you help put together...extracurricular activities – did you volunteer to do science outreach and teach children about science...

  5. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...students and postdocs when starting out. She advises “do not hurry to staff up the lab and to take things ...of great projects as ‘impossible' or useless.” How do you run your lab differently or the same as your ...

  6. 2022 Depositor Week

    Type
    Blog Post
    Published
    Dec. 12, 2022, 2:15 p.m.
    ... Specialized Depositor Shout-Out  We'll also be doing giveaways on Twitter, so keep an eye out there for...

  7. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...and vocabulary for this skill. What this post may do is help you realize whether or not you have a natural...t mean you have to like conflict. Very few people do, and it’s best to be wary of anyone who is actually...there is a potential for long-term gains when you can do it. If Party X proposes solution A, and Party Y proposes...

  8. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...increase therapeutic efficacy (Ahmed et al., 2012). How do you generate an scFv?  scFv sequences have traditionally...antibody genes from diseased or vaccinated humans. How do you produce scFvs? While it’s possible to produce...scFvs are even used to screen for new antibodies. To do this, antibody variable domain sequences are expressed...

  9. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...the chance of off-target effects, you still need to do your due diligence to ensure they are indeed a non-issue...off-targets don’t mess with your results when they do arise. Happy CRISPR’ing! References and Resources...resources on the Addgene blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? CRISPR 101: Cas9...

  10. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...observe your cells on a flow plot, you might have to do some testing or ask experienced colleagues. ...warning message at the top, as our cells of interest do not lie beyond the dimensions displayed in the plot...4). As H and A scale in proportion (while H and W do not; see Figure 2), single cell events will show ...

  12. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...process is very similar for both, though tissue samples do require an extra homogenization step. For this blog...courtesy of Shengliu via Wikicommons.   Okay, let’s do a quick review. At this point you have (1) crosslinked...

  13. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...unbound) population. The benefit of beads is that they do not require cells from your experiment, and they ...prefers to use them to gate our populations, but others do not. Some journals require isotype controls, while...

  15. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ... this given that the brain controls everything we do, but I was quite impressed by how neuroscientists...to get into the field, this is a fantastic time to do it! Additional Resources on the Addgene Blog Advanced...

  16. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...similarity. REPLACR-mutagenesis: Letting bacteria do the work If you’ve conducted SDM using some of these...on in vitro assembly, they could make the E. coli do the work by using the principles of recombineering...

  17. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...? Blue-white selection is a widely used method to do just that! (To read about other screening strategies...with this method to ensure that positive colonies do have the plasmid containing the lethal gene. An example...

  18. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ... all driven by personal connections What can you do to get the most from the time and money you are spending...about how you want to introduce yourself and what you do. You can probably use some scientific terms when ...

  19. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...unexplored areas with our color-changing flower, but to do that we’ve had to navigate the challenges involved... plant synthetic biology. We cannot build what we do not understand, and the complicated and unique metabolic...

  20. The Twelve Days of Antibodies

    Type
    Blog Post
    Published
    Jan. 6, 2025, 5:17 p.m.
    ... to our blog and social media. This year, we're doing so through the twelve days of antibodies. We hope...
Showing: 261 - 280 of 622 results