...non-trivial, however, as FPs are tremendously complicated entities with a large range of characteristics...decision remains spread across literature in publications that introduce these tools. Years ago, Kurt...collection pages can be kept private, or shared publicly (so feel free to moderate your own version of ...incorporate FPbase data in their own projects and applications, a REST API allows programmatic retrieval of...

...protein-protein interactions. Beyond these common applications, researchers are finding new ways to apply fluorescent...Zhou and Fatima Enam. There were many excellent applicants but Xin and Fatima stood out for their creativity...utility of these FLiPs in a variety of research applications and particularly notes their ability to be precisely...Update (7/17/2018): Check out Fatima's recent publication on her HMO work in Cell Chemical Biology! Additional...

...Luciola italica)Southern Russian firefly(Luciola mingrelica) 562 64 Luciferin ATP No Jamaican click beetle...which luciferases have been cloned from include click beetles (Pyrophorus plagiophthalmus), railroad worm...

...Scientists can also use electroporation, the application of an electrical charge to cells, to increase... properties. The phagemids are packaged into replication-incompetent phage particles with assistance from... found on an episome, a piece of DNA that can replicate on its own or be integrated within a bacterial...Multiple phases of competence occur during the Helicobacter pylori growth cycle." FEMS microbiology letters...

...historical standard. Specifically, in our recent publications, we used the cancer plasmid collections listed...Cancer Variants Identifies Oncogenic Alleles. Kim E, Ilic N, Shrestha Y. et al. Cancer Discovery 2016. PubMed...pathway reference collection is described in this publication We also wrote an extensive review on the exciting...data generated from these alleles in the above publications), it will be possible for many others to perform...

...cardiovascular and metabolic diseases. We are knocking out genes that have been implicated in disease or, ...to understand the basis for cardiovascular and metabolic human diseases. They do that by studying patients...less than a month's time. Addgene: What are the implications of this kit in terms of understanding human ...

...you’ll see the boxes that say “Connect.” If you click this box, it will send an unpersonalized, generic...change the option for sending LinkedIn messages to Click Send instead of Press Enter to Send. That way you...shoulder for the picture. It tends to look like a police photo if you look straight at the camera. Pro tip...not the platform for politics, family updates, or click bait of any kind. For more on inappropriate content...

...journalism.” She also interned two days a week on a public radio show. After graduating with a master’s, Susan...complete a certificate in Strategic Communications and Public Relations at the University of Washington and the...opportunities, such as writing for a student-led publication, are more readily available and it’s often easier...internship similar to Susan’s NEI internship. Application information can be found here Find tips for using...

... PMCID: PMC3918502. Find plasmids from this publication at Addgene. 2. Ma, Hanhui, et al. “Multicolor...PMCID: PMC4364232. Find the plasmids from this publication at Addgene. 3. Ma, Hanhui, et al. “Multiplexed...PMCID: PMC4864854. Find the plasmids from this publication at Addgene. 4. Kiani, Samira, et al. “Cas9 gRNA... PMCID: PMC4666719. Find plasmids from this publication at Addgene. Additional Resources on the Addgene...

...experiments. Recombinant AAVs (rAAVs) The primary application of AAV is delivery of genetic cargo to a host...transduced gene is episomal and does not divide and replicate in the same way the host DNA does. Because of ... since you don’t have to wait for the host to replicate the DNA before expression. Now that you’ve got... S., et al. “Various AAV Serotypes and Their Applications in Gene Therapy: An Overview.” Cells, 12(5),...

...aggregates. This property can be tolerated in some applications (e.g. labeling of cells or tracking promotor...promotor activity), but it is problematic in applications in which the fluorescent protein is used as an inert...During the experiment, the solution is diluted, complicating the interpretation. Because of this, these methods...aware that the fluorescent proteins reported in publications are only characterized under a limited number...

...systems are easily adaptable to neuroscience applications, including optogenetics, and they should make...Tang note that their scaffold system is likely applicable to many types of proteins. For proteins with ... PMCID: PMC4096992. Find plasmids from this publication at Addgene. 2. Tang, Jonathan C.Y., et al. “...PubMed PMID: 26258682. Find plasmids from this publication at Addgene. 3. Kirchhofer, A., et al. “Modulation...

...prevent the formation of replication competent lentivirus (RCL). Replication competence can arise from...end-users, size of the transgene, and downstream applications should all be considered when choosing the correct... as resources available, cost and downstream applications. For example, while the PEG precipitation method...

...for more complicated plasmids, like those that contain synthesized regions that have no publicly available...often find a few mismatches in the origin of replication or other common backbone elements. Since we’ve...chicken beta actin promoter, and rabbit beta-globin splice acceptor, contains sequence that is over 80% GC...

... protein interactions (Alice Ting Lab 2020) Split-horseradish peroxidase (Alice Ting Lab 2016)  Split-...Image modified from Feng et al. 2017 under CC-BY license. Many split-FP fragments are named according...Split-Fluorescent Protein Tags Browse the table below, and click the Article link to find all the plasmids from the...

... in situ hybridization experiments. A recent publication [1] from the Sánchez lab describes the plasmids...the deposit) are described in the Sánchez lab publication [1] so that the students performing these experiments...Phagocata gracillis, the process is a little more complicated. These strains are more picky eaters and refuse...easily investigate in planarians. References 1. Alice Accorsi, Monique M. Williams, Eric J. Ross, Sofia...

... become very different due to a whole genome duplication and extensive rearrangements in the S. cerevisiae...overexpression vector. This plasmid contains origins of replication and selectable markers that allow students to...functional MET genes to grow.) Students then use replica plating to determine if expression of MET/Met genes...undergraduate courses and in communicating science to the public. You can find out more about Clare at the Biology...

...setting—especially if job applications require teaching experience or high numbers of publications, which may take...people involved is staggering. The process is complicated. If you’re interested in the drug discovery process... proprietary compounds or information. Where publication is expected, the process is similar to academia...

...T4 DNA ligase. Inserts, either precloned or in amplicon form (shown above) are flanked by BsaI sites in...efficiency measurements using similarly sized Lambda amplicon inserts to control for any suppression of background...Kucera is a Principal Development Scientist of Applications & Product Development focusing on DNA cloning...Optimization of Golden Gate assembly through application of ligation sequence-dependent fidelity and bias...

... in learning more about any one of them, please click on the links throughout the text or see the “Additional...cloning. Sequence and Ligation Independent Cloning (SLIC) could also be a great choice, because the T4 polymerase...The ligation-independent methods like Gibson and SLIC are also quite efficient; although efficiency often...some of the homology based methods, like Gibson or SLIC, since one can pool the components and define their...