Plasmid Cloning by PCR
Type
Blog Post
Published
March 29, 2016, 2:30 p.m.
...DNA, a cDNA library, or by RT-PCR.
Isolate the PCR product
Isolate your PCR product from the rest of the...function in the same buffer, as this will save time later
In our example, we will use EcoRI and NotI to ...TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a final Reverse Primer sequence of 5’-...If you are amplifying from a plasmid or simple template, there is very little chance for mis-priming, ...now ready for restriction digestion. As such, the later steps in this process are the same as those discussed...