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We narrowed to 321 results for: NUC

Showing: 241 - 260 of 321 results

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Predicting Adverse Reactions to Monoclonal Antibody Drugs

Type

Blog Post

Published

Feb. 13, 2024, 2:15 p.m.

...assays with immune cell lines or peripheral blood mononuclear cells (PBMC) in 3D models of the human lymphatic...
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

Type

Blog Post

Published

June 25, 2019, 12:54 p.m.

...Microinjection requires the use of a fine needle to deliver nucleic acids to individual cells. Electroporation on the...
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

Type

Blog Post

Published

June 6, 2019, 12:41 p.m.

...differentiation. Furthermore, TALEN and ZFN require custom nucleases for each gene of interest. CRISPR-Cas9 tools have...
Plasmids 101: Screening Strategies Used in Plasmid Cloning

Type

Blog Post

Published

Sept. 15, 2020, 1:15 p.m.

...Sanger sequencing determines the precise order of nucleotides within a DNA molecule, in this case a plasmid...
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

Type

Blog Post

Published

May 26, 2020, 1:15 p.m.

...DSB is repaired by NHEJ which depending on the nucleotides inserted or deleted, can result in an in-frame...
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

Type

Blog Post

Published

July 6, 2017, 1:59 p.m.

...PubMed PMID: 27411589. 2. Todoric, Jelena, Laura Antonucci, and Michael Karin. "Targeting inflammation in...
Top Requested Lentivirus and AAV of 2016

Type

Blog Post

Published

Jan. 6, 2017, 3:56 p.m.

... to perform unique editing tasks, classic Cas9 nuclease, which introduces double stranded DNA breaks, ...
Antibodies 101: Monoclonal Antibodies

Type

Blog Post

Published

June 29, 2021, 1:15 p.m.

...antibodies. Genetic drift refers to changes in the nucleic acid sequence of the antibody-encoding genes over...
GPCRs: How Do They Work and How Do We Study Them?

Type

Blog Post

Published

Feb. 11, 2021, 2:15 p.m.

...activate expression of a luciferase reporter in the nucleus. The Roth lab showed that this platform accurately...
Viral Vectors 101: Viral Vector Elements

Type

Blog Post

Published

July 25, 2023, 1 p.m.

...proteins: they contain the matrix, capsid, and nucleocapsid viral components needed for viral packaging,...
Viral Vectors 101: Viral Applications

Type

Blog Post

Published

April 13, 2023, 1:15 p.m.

...the delivery mechanism for increasingly popular nucleic acid-based vaccines. These vectors provide several...
CasPEDIA: A Functional Classification of Cas Enzymes

Type

Blog Post

Published

Jan. 25, 2024, 2:15 p.m.

...the following information for a Cas enzyme: Nuclease properties (CasID): Cis/trans activity, PAM requirement...
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

Type

Blog Post

Published

Feb. 24, 2015, 7:50 p.m.

... and it consists of three components: 1. A nucleolytically inactive Cas9-VP64 fusion; 2. A sgRNA incorporating...
The Breast Cancer Microenvironment: A Tumor’s Backstage Team

Type

Blog Post

Published

Jan. 31, 2019, 1:32 p.m.

...itself. They deliver compounds like proteins and nucleic acids from cancer cells to the microenvironment...
Supporting Rare Disease Research with the 2020 BeHEARD Awards

Type

Blog Post

Published

Sept. 8, 2020, 2 p.m.

...signalling complex assembly, and localization to the nucleus,” says Dr. Terry Hébert, a professor and assistant...
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

Type

Blog Post

Published

April 23, 2018, 2 p.m.

...estradiol for hER or progesterone for hPR - leads to nuclear localization, binding to the appropriate promoter...
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

Type

Blog Post

Published

May 7, 2020, 1:15 p.m.

...motif (PAM) made up of 5′-NGG-3′ (“N” can be any nucleotide base, followed by 2 guanines). This limits the...
Popular Retroviral Vectors and Their Uses in Scientific Research

Type

Blog Post

Published

July 24, 2018, 12:51 p.m.

...Murine Stem Cell Virus, MSCV) each with a unique 6 nucleotide barcode. This collection can be used for in vivo...
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

Type

Blog Post

Published

May 15, 2018, 12:43 p.m.

...accumulation in subcellular compartments such as nuclear speckles. Furthermore, CRY2clust, when integrated...
5 Tips for Troubleshooting Viral Transductions

Type

Blog Post

Published

Aug. 11, 2016, 2:23 p.m.

...integration of transfected DNA into normal human cells."Nucleic acids research 18.9 (1990): 2733-2738. PubMed PMID...

Showing: 241 - 260 of 321 results

Results per page:

Predicting Adverse Reactions to Monoclonal Antibody Drugs

Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

Plasmids 101: Screening Strategies Used in Plasmid Cloning

Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

Top Requested Lentivirus and AAV of 2016

Antibodies 101: Monoclonal Antibodies

GPCRs: How Do They Work and How Do We Study Them?

Viral Vectors 101: Viral Vector Elements

Viral Vectors 101: Viral Applications

CasPEDIA: A Functional Classification of Cas Enzymes

Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

The Breast Cancer Microenvironment: A Tumor’s Backstage Team

Supporting Rare Disease Research with the 2020 BeHEARD Awards

Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

Popular Retroviral Vectors and Their Uses in Scientific Research

Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

5 Tips for Troubleshooting Viral Transductions

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